ID | 30465 |
JaLCDOI | |
フルテキストURL | |
著者 |
Nakamura, Mitsuo
Kagawa Medical School
Itano, Toshifumi
Kagawa Medical School
Yamaguchi, Fuminori
Kagawa Medical School
Mizobuchi, Masayuki
Kagawa Medical School
Tokuda, Masaaki
Kagawa Medical School
Etoh, Siji
Kagawa Medical School
Hosokawa, Kiyoshi
Kagawa Medical School
Ohmoto, Takashi
Kagawa Medical School
Hatase, Osamu
Kagawa Medical School
|
抄録 | Peptides and proteins in the extracellular space in the central nervous system were investigated in vivo using an intracerebral microdialysis probe. The molecular cut-off of the hollow fiber which was used for the probe was approximately 100 kDa. We examined recovery rates of several compounds in vitro. The recovery rates of proteins and peptides were between 7-28%, with the exceptions of substance P and insulin-like growth factor I. The recovery rates of monoamines and their metabolites were 22-40%. In in vivo studies, two major proteins with apparent molecular weights of 62 kDa and 12 kDa, and several minor proteins (28 kDa, 43 kDa, 52 kDa and 70 kDa) were detected by SDS-polyacrylamide gel electrophoresis in the dialysate from a probe implanted in the striatum of anesthetized rats. These results suggest that the newly developed, intracerebral microdialysis probe might be useful for investigating the dynamic changes of peptides and proteins in the central nervous system. |
キーワード | protein
peptide
microdialysis
extracellular space
probe
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 1990-02
|
巻 | 44巻
|
号 | 1号
|
出版者 | Okayama University Medical School
|
開始ページ | 1
|
終了ページ | 8
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
PubMed ID | |
Web of Science KeyUT |