| JaLCDOI | 10.18926/AMO/64355 |
|---|---|
| フルテキストURL | 77_1_1.pdf |
| 著者 | Nahar, Lutfun| Hagiya, Hideharu| Nada, Takahiro| Iio, Koji| Gotoh, Kazuyoshi| Matsushita, Osamu| Otsuka, Fumio| |
| 抄録 | Inducible resistance to the macrolide, lincosamide, and streptogramin B (iMLSB) antibiotic family is a latent mechanism for antimicrobial resistance in Staphylococcus aureus. We here investigated the frequency and genotypic profiles of iMLSB resistance in clindamycin (CLDM)-susceptible S. aureus isolated in Okayama University Hospital from June 2020 to June 2021. We phenotypically screened the iMLSB resistance via D-zone test and performed PCR testing for the erythromycin ribosomal methylase (erm) genes: ermA and ermC. Among 432 CLDM-susceptible S. aureus isolates, 138 (31.9%) exhibited an iMLSB-resistance phenotype, with methicillinresistant S. aureus isolates (MRSA; 61 isolates: 58.6%) exhibiting higher positivity than methicillin-sensitive S. aureus isolates (MSSA; 77 isolates: 23.5%) (p<0.001). Male patients had a higher frequency of iMLSB resistance than females (OR [95%CI]: 1.8 [1.2-2.8]; p=0.007). Genotypically, ermA predominated in both MSSA (70.1%) and MRSA (86.9%) compared to ermC (14.3% in MSSA and 11.5% in MRSA). A single strain of MRSA possessed both ermA and ermC, while 12 (15.6%) MSSA isolates were negative for both ermA and ermC, suggesting the presence of other genetic mechanisms. Collectively, these results show that approximately 33% of CLDM-susceptible S. aureus isolates at our university hospital exhibited iMLSB resistance, predominantly caused by ermA in both MSSA and MRSA. |
| キーワード | antimicrobial resistance clindamycin erm D-zone test inducible MLSB |
| Amo Type | Original Article |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2023-02 |
| 巻 | 77巻 |
| 号 | 1号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 1 |
| 終了ページ | 9 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | Copyright Ⓒ 2023 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 36849140 |
| Web of Science KeyUT | 000953663800001 |
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Nakamura, Shin| Ito, Takashi| Okamoto, Kentaro| Mima, Takehiko| Uchida, Kentaro| Siddiqui, Yasir D.| Ito, Masahiro| Tai, Masako| Okubo, Keisuke| Yamashiro, Keisuke| Omori, Kazuhiro| Yamamoto, Tadashi| Matsushita, Osamu| Takashiba, Shogo| |
| キーワード | bone regeneration collagen drug delivery systems growth factors periodontitis tissue engineering |
| 発行日 | 2019-03-19 |
| 出版物タイトル | Journal of Periodontology |
| 巻 | 90巻 |
| 号 | 9号 |
| 出版者 | Wiley |
| 開始ページ | 1043 |
| 終了ページ | 1052 |
| ISSN | 0022-3492 |
| NCID | AA00704417 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2019 The Authors |
| 論文のバージョン | publisher |
| PubMed ID | 30889294 |
| DOI | 10.1002/JPER.18-0674 |
| Web of Science KeyUT | 000485288400012 |
| 関連URL | isVersionOf https://doi.org/10.1002/JPER.18-0674 |
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Kobatake, Tomomi| Ogino, Keiki| Sakae, Hiroyuki| Gotoh, Kazuyoshi| Watanabe, Akari| Matsushita, Osamu| Okada, Hiroyuki| Yokota, Kenji| |
| キーワード | disulfiram Helicobacter pylori urease vacuolating toxin CagA |
| 発行日 | 2021-05-12 |
| 出版物タイトル | Infection and Drug Resistance |
| 巻 | 14巻 |
| 出版者 | Dove Medical Press Ltd |
| 開始ページ | 1757 |
| 終了ページ | 1764 |
| ISSN | 1178-6973 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2021 Kobatake et al. |
| 論文のバージョン | publisher |
| PubMed ID | 34012274 |
| NAID | 120007042392 |
| DOI | 10.2147/IDR.S299177 |
| Web of Science KeyUT | 000653055700001 |
| 関連URL | isVersionOf https://doi.org/10.2147/IDR.S299177 |
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Ichihara, Aina| Ojima, Hinako| Gotoh, Kazuyoshi| Matsushita, Osamu| Take, Susumu| Okada, Hiroyuki| Watanabe, Akari| Yokota, Kenji| |
| キーワード | antibody VacA CagA genome |
| 発行日 | 2021-07-05 |
| 出版物タイトル | toxins |
| 巻 | 13巻 |
| 号 | 7号 |
| 出版者 | MDPI |
| 開始ページ | 467 |
| ISSN | 2072-6651 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2021 by the authors. |
| 論文のバージョン | publisher |
| DOI | 10.3390/toxins13070467 |
| Web of Science KeyUT | 000677052200001 |
| 関連URL | isVersionOf https://doi.org/10.3390/toxins13070467 |
| フルテキストURL | fulltext20211109-5.pdf |
|---|---|
| 著者 | Gotoh, Kazuyoshi| Mayura, I. Putu Bayu| Enomoto, Yusaku| Iio, Koji| Matsushita, Osamu| Otsuka, Fumio| Hagiya, Hideharu| |
| キーワード | Antimicrobial Resistance Daptomycin resistance Corynebacterium striatum Insertion sequence pgsA2 gene |
| 備考 | This is an Accepted Manuscript of an article published by Springer. This fulltext is available in Oct. 2022.| |
| 発行日 | 2021-10-21 |
| 出版物タイトル | European Journal of Clinical Microbiology & Infectious Diseases |
| 巻 | 41巻 |
| 号 | 2号 |
| 出版者 | Springer Science and Business Media LLC |
| 開始ページ | 331 |
| 終了ページ | 333 |
| ISSN | 0934-9723 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature |
| 論文のバージョン | author |
| PubMed ID | 34671843 |
| DOI | 10.1007/s10096-021-04369-1 |
| Web of Science KeyUT | 000709253000001 |
| 関連URL | isVersionOf https://doi.org/10.1007/s10096-021-04369-1 |
| フルテキストURL | fulltext20211206-1.pdf |
|---|---|
| 著者 | Gotoh, Kazuyoshi| Miyoshi, Makoto| Mayura, I Putu Bayu| Iio, Koji| Matsushita, Osamu| Otsuka, Fumio| Hagiya, Hideharu| |
| 備考 | This is an Accepted Manuscript published by Microbiology Society.| |
| 発行日 | 2021-10-4 |
| 出版物タイトル | Journal of Medical Microbiology |
| 巻 | 70巻 |
| 号 | 10号 |
| 出版者 | Microbiology Society |
| ISSN | 0022-2615 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2021 Microbiology Society |
| 論文のバージョン | author |
| PubMed ID | 34605760 |
| DOI | 10.1099/jmm.0.001430 |
| Web of Science KeyUT | 000718022100014 |
| 関連URL | isVersionOf https://doi.org/10.1099/jmm.0.001430 |
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Ojima, Hinako| Kuraoka, Sakiko| Okanoue, Shyoutarou| Okada, Hiroyuki| Gotoh, Kazuyoshi| Matsushita, Osamu| Watanabe, Akari| Yokota, Kenji| |
| キーワード | Helicobacter pylori nitrate-reducing bacteria IL-8 TNF-alpha cell cycle |
| 発行日 | 2022-12-16 |
| 出版物タイトル | Microorganisms |
| 巻 | 10巻 |
| 号 | 12号 |
| 出版者 | MDPI |
| 開始ページ | 2495 |
| ISSN | 2076-2607 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2022 by the authors. |
| 論文のバージョン | publisher |
| PubMed ID | 36557748 |
| DOI | 10.3390/microorganisms10122495 |
| Web of Science KeyUT | 000903731600001 |
| 関連URL | isVersionOf https://doi.org/10.3390/microorganisms10122495 |
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Yagisawa, Takuya| Uchiyama, Jumpei| Takemura-Uchiyama, Iyo| Ando, Shun| Ichii, Osamu| Murakami, Hironobu| Matsushita, Osamu| Katagiri, Seiji| |
| キーワード | dairy cows low fertility uterine microbiota microbial diversity bacterial association |
| 発行日 | 2023-04-26 |
| 出版物タイトル | Microbiology Spectrum |
| 巻 | 11巻 |
| 号 | 3号 |
| 出版者 | American Society for Microbiology |
| 開始ページ | e04764-22 |
| ISSN | 2165-0497 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2023 Yagisawa et al. |
| 論文のバージョン | publisher |
| PubMed ID | 37098918 |
| DOI | 10.1128/spectrum.04764-22 |
| Web of Science KeyUT | 000975042800001 |
| 関連URL | isVersionOf https://doi.org/10.1128/spectrum.04764-22 |
| フルテキストURL | fulltext20220830-2.pdf table20220830-2pdf.pdf Figs20220830-2.pdf Supplementary_data.pdf |
|---|---|
| 著者 | Uchiyama, Jumpei| Takemura-Uchiyama, Iyo| Gotoh, Kazuyoshi| Kato, Shin-ichiro| Sakaguchi, Yoshihiko| Murakami, Hironobu| Fukuyama, Tomoki| Kaneki, Mao| Matsushita, Osamu| Matsuzaki, Shigenobu| |
| キーワード | Environmental virus Jumbophage Metagenomics Evolution Uncultured phage |
| 備考 | © 2022 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 License. http://creativecommons.org/licenses/by-nc-nd/4.0/. This is the accepted manuscript version. The formal published version is available at [https://doi.org/10.1016/j.virusres.2022.198881] . | |
| 発行日 | 2022-10-02 |
| 出版物タイトル | Virus Research |
| 巻 | 319巻 |
| 出版者 | Elsevier BV |
| 開始ページ | 198881 |
| ISSN | 0168-1702 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2022 Elsevier B.V. |
| 論文のバージョン | author |
| PubMed ID | 35934259 |
| DOI | 10.1016/j.virusres.2022.198881 |
| Web of Science KeyUT | 000841172200001 |
| 関連URL | isVersionOf https://doi.org/10.1016/j.virusres.2022.198881 |
| JaLCDOI | 10.18926/AMO/49252 |
|---|---|
| フルテキストURL | 67_1_9.pdf |
| 著者 | Fatmawati, Ni Nengah Dwi| Sakaguchi, Yoshihiko| Suzuki, Tomonori| Oda, Masataka| Shimizu, Kenta| Yamamoto, Yumiko| Sakurai, Jun| Matsushita, Osamu| Oguma, Keiji| |
| 抄録 | Clostridium botulinum type C and D strains recently have been found to produce PLC on egg yolk agar plates. To characterize the gene, enzymatic and biological activities of C. botulinum PLCs (Cb-PLCs), the cb-plc genes from 8 strains were sequenced, and 1 representative gene was cloned and expressed as a recombinant protein. The enzymatic and hemolytic activities of the recombinant Cb-PLC were measured and compared with those of the Clostridium perfringens alpha-toxin. Each of the eight cb-plc genes encoded a 399 amino acid residue protein preceded by a 27 residue signal peptide. The protein consists of 2 domains, the N- and C-domains, and the overall amino acid sequence identity between Cb-PLC and alpha-toxin was greater than 50%, suggesting that Cb-PLC is homologous to the alpha-toxin. The key residues in the N-domain were conserved, whereas those in the C-domain which are important in membrane interaction were different than in the alpha-toxin. As expected, Cb-PLC could hydrolyze egg yolk phospholipid, p-nitrophenylphosphorylcholine, and sphingomyelin, and also exhibited hemolytic activity;however, its activities were about 4- to over 200-fold lower than those of alpha-toxin. Although Cb-PLC showed weak enzymatic and biological activities, it is speculated that Cb-PLC might play a role in the pathogenicity of botulism or for bacterial survival. |
| キーワード | botulinum phospholipase C botulinum toxin phospholipase C activity sphingomyelinase activity hemolytic activity |
| Amo Type | Original Article |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2013-02 |
| 巻 | 67巻 |
| 号 | 1号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 9 |
| 終了ページ | 18 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | CopyrightⒸ 2013 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 23439504 |
| Web of Science KeyUT | 000316829900002 |
| 関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/49731 |
| JaLCDOI | 10.18926/AMO/49667 |
|---|---|
| フルテキストURL | 67_2_93.pdf |
| 著者 | Kita, Masahide| Yokota, Kenji| Okada, Hiroyuki| Take, Susumu| Takenaka, Ryuta| Kawahara, Yoshiro| Oguma, Keiji| Matsushita, Osamu| Yamamoto, Kazuhide| |
| 抄録 | Atrophy of the gastric mucosa is a precursor of intestinal-type gastric cancer, and Helicobacter pylori infection causes atrophic gastritis. The aim of this study was to determine whether the genetic diversity of H. pylori virulence genes is associated with the development and progression of gastric atrophy in humans. We isolated and cultured H. pylori strains from patients with gastric ulcer and duodenal ulcer accompanied by atrophic gastritis in background mucosa. H. pylori strains were stored at －80℃ prior to the experiments being carried out. We analyzed iceA, babA, vacA, cagA, and cagE genes by PCR. The cagA gene was analyzed through sequencing of the C-terminal region containing the EPIYA motif, which is related to tyrosine phosphorylation. Severe atrophy was observed in patients with gastric ulcer. The major phenotype of the vacA gene was s1c/m1 (93ｵ). The cagA gene was detected in all strains. The cagE gene was not detected in 2 and 5 strains from the mild cases and severe cases, respectively. The major cagA EPIYA motif, which is amino acids repeat in the C terminus, was the A-B-D type (44 of 58 strains). The virulence genes were not statistically associated with the severity of atrophy in the background gastric mucosa in humans. Not only identification of bacterial virulence factors but also studies of the host response will be necessary to investigate the progression of gastric atrophy and subsequent cancer development in humans. |
| キーワード | Helicobacter pylori virulence genes chronic atrophic gastritis |
| Amo Type | Original Article |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2013-04 |
| 巻 | 67巻 |
| 号 | 2号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 93 |
| 終了ページ | 98 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | CopyrightⒸ 2013 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 23603925 |
| Web of Science KeyUT | 000317801700003 |
| 関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/52508 |
| フルテキストURL | fulltext20221116-2.pdf |
|---|---|
| 著者 | Gotoh, Kazuyoshi| Hagiya, Hideharu| Iio, Koji| Yamada, Haruto| Matsushita, Osamu| Otsuka, Fumio| |
| キーワード | Antimicrobial resistance Carbapenemase-producing Enterobacterales Carbapenem-resistant Enterobacterales New Delhi metallo-β-lactamase (NDM) Enterobacter cloacae complex |
| 備考 | © 2022 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. This manuscript version is made available under the CC-BY-NC-ND 4.0 License. http://creativecommons.org/licenses/by-nc-nd/4.0/. This is the accepted manuscript version. The formal published version is available at [https://doi.org/10.1016/j.jiac.2022.08.019] .| This full-text will be available in Oct. 2023.| |
| 発行日 | 2022-12 |
| 出版物タイトル | Journal of Infection and Chemotherapy |
| 巻 | 28巻 |
| 号 | 12号 |
| 出版者 | Elsevier BV |
| 開始ページ | 1697 |
| 終了ページ | 1699 |
| ISSN | 1341-321X |
| NCID | AA11057978 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2022 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. |
| 論文のバージョン | author |
| PubMed ID | 36049614 |
| DOI | 10.1016/j.jiac.2022.08.019 |
| Web of Science KeyUT | 000874559900019 |
| 関連URL | isVersionOf https://doi.org/10.1016/j.jiac.2022.08.019 |