フルテキストURL J. Biol. Chem_292_9_3909.pdf
著者 Takeuchi, Tomoya| Harada, Yuika| Moriyama, Satomi| Furuta, Kazuyuki| Tanaka, Satoshi| Miyaji Takaaki| Omote, Hiroshi| Moriyama, Yoshinori| Hiasa, Miki|
抄録 Mast cells are secretory cells that play an important role in host defense by discharging various intragranular contents, such as histamine and serotonin, upon stimulation of Fc receptors. The granules also contain spermine and spermidine, which can act as modulators of mast cell function, although the mechanism underlying vesicular storage remains unknown. Vesicular polyamine transporter (VPAT), the fourth member of the SLC18 transporter family, is an active transporter responsible for vesicular storage of spermine and spermidine in neurons. In the present study, we investigated whether VPAT functions in mast cells. RT-PCR and Western blotting indicated VPAT expression in murine bone marrow-derived mast cells (BMMCs). Immunohistochemical analysis indicated that VPAT is colocalized with VAMP3 but not with histamine, serotonin, cathepsin D, VAMP2, or VAMP7. Membrane vesicles from BMMCs accumulated spermidine upon the addition of ATP in a reserpine- and bafilomycin A1-sensitive manner. BMMCs secreted spermine and spermidine upon the addition of either antigen or A23187 in the presence of Ca2+, and the antigen-mediated release, which was shown to be temperature-dependent and sensitive to bafilomycin A1 and tetanus toxin, was significantly suppressed by VPAT gene RNA interference. Under these conditions, expression of vesicular monoamine transporter 2 was unaffected, but antigen-dependent histamine release was significantly suppressed, which was recovered by the addition of 1 mm spermine. These results strongly suggest that VPAT is expressed and is responsible for vesicular storage of spermine and spermidine in novel secretory granules that differ from histamine- and serotonin-containing granules and is involved in vesicular release of these polyamines from mast cells.
キーワード histamine mast cell polyamine secretory granules spermine transporter vesicles vesicular polyamine transporter
発行日 2017-03
出版物タイトル Journal of Biological Chemistry
292巻
9号
出版者 American Society for Biochemistry and Molecular Biology
開始ページ 3909
終了ページ 3918
ISSN 0021-9258
NCID AA00251083
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン publisher
PubMed ID 28082679
DOI 10.1074/jbc.M116.756197
Web of Sience KeyUT 000395542500031
関連URL isVersionOf https://doi.org/10.1074/jbc.M116.756197
著者 Miyaji, Takaaki| Kuromori, Takashi| Takeuchi, Yu| Yamaji, Naoki| Yokosho, Kengo| Shimazawa, Atsushi| Sugimoto, Eriko| Omote, Hiroshi| Ma, Jian Feng| Shinozaki, Kazuo| Moriyama, Yoshinori|
発行日 2015-01-05
出版物タイトル Nature Communications
6巻
資料タイプ 学術雑誌論文
著者 Hiasa, Miki| Miyaji, Takaaki| Haruna, Yuka| Takeuchi, Tomoya| Harada, Yuika| Moriyama, Sawako| Yamamoto, Akitsugu| Omote, Hiroshi| Moriyama, Yoshinori|
発行日 2014-10-30
出版物タイトル Scientific Reports
4巻
資料タイプ 学術雑誌論文
著者 Sawada, Keisuke| Echigo, Noriko| Juge, Narinobu| Miyaji, Takaaki| Otsuka, Masato| Omote, Hiroshi| Yamamoto, Akitsugu| Moriyama, Yoshinori|
発行日 2008-04-01
出版物タイトル Proceedings of the National Academy of Sciences of the United States of America
105巻
15号
資料タイプ 学術雑誌論文
著者 Morimoto, Riyo| Uehara, Shunsuke| Yatsushiro, Shouki| Juge, Narinobu| Hua, Zhaolin| Senoh, Shigenori| Echigo, Noriko| Hayashi, Mitsuko| Mizoguchi, Toshihide| Ninomiya, Tadashi| Udagawa, Nobuyuki| Omote, Hiroshi| Yamamoto, Akitsugu| Edwards, Robert H| Moriyama, Yoshinori|
発行日 2006-09-20
出版物タイトル EMBO Journal
25巻
18号
資料タイプ 学術雑誌論文
著者 Otsuka, Masato| Matsumoto, Takuya| Morimoto, Riyo| Arioka, Shigeo| Omote, Hiroshi| Moriyama, Yoshinori|
発行日 2007-09-25
出版物タイトル Proceedings of the National Academy of Sciences of the United States of America
102巻
50号
資料タイプ 学術雑誌論文
著者 Futai, Masamitsu| Oka, Toshihiko| Sun-Wada, Ge-hong| Moriyama, Yoshinori| Kanazawa, Hiroshi| Wada, Yoh|
発行日 2000-01
出版物タイトル The Journal of Experimental Biology
203巻
1号
資料タイプ 学術雑誌論文
著者 Moriyama, Yoshinori| Hayashi, Mitsuko| Yamada, Hiroshi| Yatsushiro, Shouki| Ishio, Shougo| Yamamoto, Akitsugu|
発行日 2001-01
出版物タイトル The Journal of Experimental Biology
203巻
1号
資料タイプ 学術雑誌論文