| 著者 | Murakami, Jun| Asaumi, Jun-ichi| Kawai, Noriko| Tsujigiwa, Hidetsugu| Yanagi, Yoshinobu| Nagatsuka, Hitoshi| Inoue, Tetsuyoshi| Kokeguchi, Susumu| Kawasaki, Shoji| Kuroda, Masahiro| Tanaka, Noriaki| Matsubara, Nagahide| Kishi, Kanji| |
|---|---|
| 発行日 | 2005-07 |
| 出版物タイトル | Cancer Chemotherapy and Pharmacology |
| 巻 | 56巻 |
| 号 | 1号 |
| 資料タイプ | 学術雑誌論文 |
| JaLCDOI | 10.18926/AMO/48566 |
|---|---|
| フルテキストURL | 66_3_263.pdf |
| 著者 | Sasaki, Takanori| Kuroda, Masahiro| Katashima, Kazunori| Ashida, Masakazu| Matsuzaki, Hidenobu| Asaumi, Junichi| Murakami, Jun| Ohno, Seiichiro| Kato, Hirokazu| Kanazawa, Susumu| |
| 抄録 | The roles of cell density, extracellular space, intracellular factors, and apoptosis induced by the molecularly targeted drug rituximab on the apparent diffusion coefficient (ADC) values were investigated using bio-phantoms. In these bio-phantoms, Ramos cells (a human Burkittｾs lymphoma cell line) were encapsulated in gellan gum. The ADC values decreased linearly with the increase in cell density, and declined steeply when the extracellular space became less than 4 μm. The analysis of ADC values after destruction of the cellular membrane by sonication indicated that approximately 65% of the ADC values of normal cells originate from the cell structures made of membranes and that the remaining 35% originate from intracellular components. Microparticles, defined as particles smaller than the normal cells, increased in number after rituximab treatments, migrated to the extracellular space and significantly decreased the ADC values of bio-phantoms during apoptosis. An in vitro study using bio-phantoms was conducted to quantitatively clarify the roles of cellular factors and of extracellular space in determining the ADC values yielded by tumor cells and the mechanism by which apoptosis changes those values. |
| キーワード | apparent diffusion coefficient value cell density extracellular space bio-phantom |
| Amo Type | Original Article |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2012-06 |
| 巻 | 66巻 |
| 号 | 3号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 263 |
| 終了ページ | 270 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | CopyrightⒸ 2012 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 22729107 |
| Web of Science KeyUT | 000305669700010 |
| 著者 | Murakami, Jun| Asaumi, Jun-ichi| Maki, Yuu| Tsujigiwa, Hidetsugu| Kuroda, Masahiro| Nagai, Noriyuki| Yanagi, Yoshinobu| Inoue, Tetsuyoshi| Kawasaki, Shoji| Tanaka, Noriaki| Matsubara, Nagahide| Kishi, Kanji| |
|---|---|
| 発行日 | 2004-2 |
| 出版物タイトル | Oral Oncology |
| 巻 | 40巻 |
| 号 | 6号 |
| 資料タイプ | 学術雑誌論文 |
| JaLCDOI | 10.18926/AMO/52009 |
|---|---|
| フルテキストURL | 67_6_359.pdf |
| 著者 | Katashima, Kazunori| Kuroda, Masahiro| Ashida, Masakazu| Sasaki, Takanori| Taguchi, Takehito| Matsuzaki, Hidenobu| Murakami, Jun| Yanagi, Yoshinobu| Hisatomi, Miki| Hara, Marina| Kato, Hirokazu| Ohmura, Yuichi| Kobayashi, Tomoki| Kanazawa, Susumu| Harada, Sosuke| Takemoto, Mitsuhiro| Ohno, Seiichiro| Mimura, Seiichi| Asaumi, Junichi| |
| 抄録 | It is well known that many tumor tissues show lower apparent diffusion coefficient (ADC) values, and that several factors are involved in the reduction of ADC values. The aim of this study was to clarify how much each factor contributes to decreases in ADC values. We investigate the roles of cell density, extracellular space, intracellular factors, apoptosis and necrosis in ADC values using bio-phantoms. The ADC values of bio-phantoms, in which Jurkat cells were encapsulated by gellan gum, were measured by a 1.5-Tesla magnetic resonance imaging device with constant diffusion time of 30sec. Heating at 42℃ was used to induce apoptosis while heating at 48℃ was used to induce necrosis. Cell death after heating was evaluated by flow cytometric analysis and electron microscopy. The ADC values of bio-phantoms including non-heated cells decreased linearly with increases in cell density, and showed a steep decline when the distance between cells became less than 3μm. The analysis of ADC values of cells after destruction of cellular structures by sonication suggested that approximately two-thirds of the ADC values of cells originate from their cellular structures. The ADC values of bio-phantoms including necrotic cells increased while those including apoptotic cells decreased. This study quantitatively clarified the role of the cellular factors and the extracellular space in determining the ADC values produced by tumor cells. The intermediate diffusion time of 30msec might be optimal to distinguish between apoptosis and necrosis. |
| キーワード | ADC apoptosis necrosis hyperthermia cell density |
| Amo Type | Original Article |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2013-12 |
| 巻 | 67巻 |
| 号 | 6号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 359 |
| 終了ページ | 367 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | CopyrightⒸ 2013 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 24356720 |
| Web of Science KeyUT | 000328915700004 |
| 著者 | Maki, Yu| Murakami, Jun| Asaumi, Jun-ichi| Tsujigiwa, Hidetsugu| Nagatsuka, Hitoshi| Kokeguchi, Susumu| Fukui, Kazuhiro| Kawai, Noriko| Yanagi, Yoshinobu| Kuroda, Masahiro| Tanaka, Noriaki| Matsubara, Nagahide| Kishi, Kanji| |
|---|---|
| 発行日 | 2005-7 |
| 出版物タイトル | Oral Oncology |
| 巻 | 41巻 |
| 号 | 10号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 石井 龍宏| 村上 純| 能登原 憲司| 笹本 博美| 神原 健| 白川 靖博| 猶本 良夫| 大内田 守| 清水 憲二| Jeremy, R. Jass| 松原 長秀| 田中 紀章| |
|---|---|
| 発行日 | 2007-09-03 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 119巻 |
| 号 | 2号 |
| 資料タイプ | 学術雑誌論文 |