start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=11
article-no=
start-page=3653
end-page=3665
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=2025
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Survey of Barley Sodium Transporter HvHKT1;1 Variants and Their Functional Analysis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Barley (Hordeum vulgare L.) employs the Na+ transporter HvHKT1;1, which is an N+-selective transporter. This study characterized the full-length HvHKT1;1 (HvHKT1;1-FL) and three mRNA variants (HvHKT1;1-V1, -V2, and -V3), which encode polypeptides of 64.7, 54.0, 40.5, and 32.9 kDa, respectively. Tissue-specific expression profiling revealed that HvHKT1;1-FL is the most abundant transcript across leaf, sheath, and root tissues under normal conditions, with the highest expression in leaves. Under 150 mM NaCl stress, HvHKT1;1-FL and its variants showed a dynamic, time-dependent expression pattern, with peak leaf expression at 2 h, sheath expression at 12 h, and root expression at 2 h, suggesting their roles in early stress response. Functional analysis using two-electrode voltage-clamp measurements demonstrated that HvHKT1;1-FL is highly selective for Na+, with minimal conductance for K+, Li+, Rb+, or Cs+. It demonstrated high Na+ transport efficiency, characterized by higher Vmax and lower Km values, while the variants showed reduced Na+ currents, lower Vmax, and higher Km values, indicating decreased Na+ transport capacity. Reversal potential analyses further confirmed Na+ selectivity, with HvHKT1;1-FL displaying the strongest preference for Na+. Notably, while all variants retained Na+ selectivity, they showed reduced efficiency, as indicated by a more negative reversal potential in low Na+ conditions. These findings highlight the functional diversity among HvHKT1;1 variants, with HvHKT1;1-FL playing a dominant role in Na+ transport. The tissue-specific regulation of these variants under salinity stress underscores their importance in barley’s adaptive responses.
en-copyright=
kn-copyright=

en-aut-name=ImranShahin
en-aut-sei=Imran
en-aut-mei=Shahin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KatsuharaMaki
en-aut-sei=Katsuhara
en-aut-mei=Maki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Agronomy, Khulna Agricultural University
kn-affil=
en-keyword=Barley
kn-keyword=Barley
en-keyword=HvHKT1;1
kn-keyword=HvHKT1;1
en-keyword=Na+ transport
kn-keyword=Na+ transport
en-keyword=mRNA variants
kn-keyword=mRNA variants
en-keyword=TEVC
kn-keyword=TEVC
END

start-ver=1.4
cd-journal=joma
no-vol=66
cd-vols=
no-issue=7
article-no=
start-page=1044
end-page=1060
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250527
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Oxygen supply is a prerequisite for response to aluminum in cultured cells of tobacco (Nicotiana tabacum)
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Responses to aluminum (Al) were investigated in tobacco cells (cell line SL) in a calcium-sucrose solution for up to 24 h under shaking (aerobic) condition. Microarray analysis of upregulated and downregulated genes under Al exposure and following Gene Ontology (GO) enrichment analysis of biological process category revealed only one GO term to be enriched for the upregulated genes, “response to chitin,” annotated with genes encoding transcription factors (NtERF1 and NtMYB3) and MAP kinase (WIPK), and nine GO terms for the downregulated genes, including “cell wall loosening” and “lipid transport,” annotated with genes encoding expansin (NtEXPA4) and lipid transfer protein (LTP)/LTP-like (NtLTP3 and NtEIG-C29), respectively. Al triggered the production of nitric oxide (NO) then reactive oxygen species (ROS). Addition of NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide decreased the levels of NO and a part of the transcriptional changes described above, but increased the levels of ROS and a loss of growth capacity, suggesting a role of the NO to induce the transcriptional changes partly and to repress these toxic responses under Al exposure. Under non-shaking (anaerobic) condition, the cells exhibited upregulation of several hypoxia-responsive genes. The cells exposed to Al exhibited the same level of Al accumulation but much lower levels of the Al responses including NO production, ROS production, a loss of growth capacity, citrate secretion, and a part of the transcriptional changes described above, compared with the cells under shaking condition. These results suggest that coexistence of oxygen with Al is necessary to trigger the Al responses related to toxicity and tolerance.
en-copyright=
kn-copyright=

en-aut-name=TsuchiyaYoshiyuki
en-aut-sei=Tsuchiya
en-aut-mei=Yoshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KatsuharaMaki
en-aut-sei=Katsuhara
en-aut-mei=Maki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SasakiTakayuki
en-aut-sei=Sasaki
en-aut-mei=Takayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YamamotoYoko
en-aut-sei=Yamamoto
en-aut-mei=Yoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Institute of Plant Science and Resources, Okayama University 
kn-affil=

affil-num=2
en-affil=Institute of Plant Science and Resources, Okayama University 
kn-affil=

affil-num=3
en-affil=Institute of Plant Science and Resources, Okayama University 
kn-affil=

affil-num=4
en-affil=Institute of Plant Science and Resources, Okayama University 
kn-affil=
en-keyword=aluminum toxicity
kn-keyword=aluminum toxicity
en-keyword=aluminum-responsive genes
kn-keyword=aluminum-responsive genes
en-keyword=cell wall loosening
kn-keyword=cell wall loosening
en-keyword=chitin-responsive genes
kn-keyword=chitin-responsive genes
en-keyword=dioxygen
kn-keyword=dioxygen
en-keyword=hypoxia
kn-keyword=hypoxia
END

start-ver=1.4
cd-journal=joma
no-vol=14
cd-vols=
no-issue=17
article-no=
start-page=1305
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250822
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Discovery and Functional Characterization of Novel Aquaporins in Tomato (Solanum lycopersicum): Implications for Ion Transport and Salinity Tolerance
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Aquaporins (AQPs) are membrane proteins that facilitate the transport of water and solutes. Among AQPs, plasma membrane intrinsic proteins (PIPs) play a critical role in maintaining water balance between the internal and external cell environments. This study focuses on the tomato due to its economic importance and cultivation under moderate salinity conditions in Japan. A swelling assay using X. laevis oocyte confirmed that all five examined tomato SlPIP2 isoforms showed water transport activity. Among them, two-electrode voltage clamp (TEVC) experiments showed that only SlPIP2;1, SlPIP2;4, and SlPIP2;8 transport Na+ and K+, with no transport activity for Cs+, Rb+, Li+, or Cl−. CaCl2 (1.8 mM) reduced ionic currents by approximately 45% compared to 30 µM free-Ca2+. These isoforms function as very low-affinity Na+ and K+ transporters. Expression analysis showed that SlPIP2;4 and SlPIP2;8 had low, stable expression, while SlPIP2;1 was strongly upregulated in roots NaCl treatment (200 mM, 17days), suggesting distinct physiological roles for these ion-conducting AQPs (icAQPs). These data hypothesized that tomato icAQPs play a critical role in ion homeostasis, particularly under salinity stress. In conclusion, the first icAQPs have been identified in the dicotyledonous crop. These icAQPs are essential for plant resilience under salt stress.
en-copyright=
kn-copyright=

en-aut-name=PaulNewton Chandra
en-aut-sei=Paul
en-aut-mei=Newton Chandra
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ImranShahin
en-aut-sei=Imran
en-aut-mei=Shahin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MitsumotoAnri
en-aut-sei=Mitsumoto
en-aut-mei=Anri
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MoriIzumi C.
en-aut-sei=Mori
en-aut-mei=Izumi C.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KatsuharaMaki
en-aut-sei=Katsuhara
en-aut-mei=Maki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=2
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=3
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=4
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=5
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=
en-keyword=Aquaporin (AQP)
kn-keyword=Aquaporin (AQP)
en-keyword=ion transport
kn-keyword=ion transport
en-keyword=plasma membrane intrinsic proteins (PIPs)
kn-keyword=plasma membrane intrinsic proteins (PIPs)
en-keyword=tomato
kn-keyword=tomato
en-keyword=oocytes
kn-keyword=oocytes
en-keyword=water transport
kn-keyword=water transport
END

start-ver=1.4
cd-journal=joma
no-vol=15
cd-vols=
no-issue=1
article-no=
start-page=12857
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250414
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=OsPIP2;4 aquaporin water channel primarily expressed in roots of rice mediates both water and nonselective Na+ and K+ conductance
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Aquaporin (AQP)-dependent water transport across membranes is indispensable in plants. Recent evidence shows that several AQPs, including plasma membrane intrinsic proteins (PIPs), facilitate the electrogenic transport of ions as well as water transport and are referred to as ion-conducting aquaporins (icAQPs). The present study attempted to identify icAQPs that exhibit cation transport activity among PIPs from rice. Electrophysiological experiments on 11 OsPIPs using Xenopus laevis oocytes revealed that OsPIP2;4 mediated the electrogenic transport of alkali monovalent cations with the selectivity sequence of Na+ ≈ K+ > Rb+ > Cs+ > Li+, suggesting non-selective cation conductance for Na+ and K+. Transcripts of OsPIP2;4 were abundant in the elongation and mature zones of roots with similar expression levels between the root stelar and remaining outer parts in the cultivar Nipponbare. Immunostaining using sections of the crown roots of Nipponbare plants revealed the expression of OsPIP2;4 in the exodermis and sclerenchyma of the surface region and in the endodermis and pericycle of the stelar region. The present results provide novel insights into OsPIP2;4-dependent non-selective Na+ and K+ transport and its physiological roles in rice.
en-copyright=
kn-copyright=

en-aut-name=TranSen Thi Huong
en-aut-sei=Tran
en-aut-mei=Sen Thi Huong
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KatsuharaMaki
en-aut-sei=Katsuhara
en-aut-mei=Maki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MitoYunosuke
en-aut-sei=Mito
en-aut-mei=Yunosuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OnishiAya
en-aut-sei=Onishi
en-aut-mei=Aya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=HigaAyaka
en-aut-sei=Higa
en-aut-mei=Ayaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=OnoShuntaro
en-aut-sei=Ono
en-aut-mei=Shuntaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=PaulNewton Chandra
en-aut-sei=Paul
en-aut-mei=Newton Chandra
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=HorieRie
en-aut-sei=Horie
en-aut-mei=Rie
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=HaradaYoshihiko
en-aut-sei=Harada
en-aut-mei=Yoshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=HorieTomoaki
en-aut-sei=Horie
en-aut-mei=Tomoaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

affil-num=1
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=2
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=3
en-affil=Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
kn-affil=

affil-num=4
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=5
en-affil=Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
kn-affil=

affil-num=6
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=7
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=8
en-affil=Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
kn-affil=

affil-num=9
en-affil=Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
kn-affil=

affil-num=10
en-affil=Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
kn-affil=
en-keyword=Ion-conducting Aquaporins
kn-keyword=Ion-conducting Aquaporins
en-keyword=Non-selective cation channel
kn-keyword=Non-selective cation channel
en-keyword=Rice
kn-keyword=Rice
en-keyword=Roots
kn-keyword=Roots
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250603
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Amino Acid Substitutions in Loop C of Arabidopsis PIP2 Aquaporins Alters the Permeability of CO2
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The transport of CO2 across biomembranes in plant cells is essential for efficient photosynthesis. Some aquaporins capable of CO2 transport, referred to as ‘COOporins’, are postulated to play a crucial role in leaf CO2 diffusion. However, the structural basis of CO2 permeation through aquaporins remains largely unknown. Here, we show that amino acids in loop C are critical for the CO2 permeability of Arabidopsis thaliana PIP2 aquaporins. We found that swapping tyrosine and serine in loop C to histidine and phenylalanine, which differ between AtPIP2;1 and AtPIP2;3, altered CO2 permeability when examined in the Xenopus laevis oocyte heterologous expression system. AlphaFold2 modelling indicated that these substitution induced a conformational shift in the sidechain of arginine in the aromatic/arginine (ar/R) selectivity filter and in lysine at the extracellular mouth of the monomeric pore in PIP2 aquaporins. Our findings demonstrate that distal amino acid substitutions can trigger conformational changes of the ar/R filter in the monomeric pore, modulating CO2 permeability. Additionally, phylogenetic analysis suggested that aquaporins capable of dual water/CO2 permeability are ancestral to those that are water-selective and CO2-impermeable, and CO2-selective and water impermeable.
en-copyright=
kn-copyright=

en-aut-name=TaniaShaila Shermin
en-aut-sei=Tania
en-aut-mei=Shaila Shermin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=UtsugiShigeko
en-aut-sei=Utsugi
en-aut-mei=Shigeko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TsuchiyaYoshiyuki
en-aut-sei=Tsuchiya
en-aut-mei=Yoshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SasanoShizuka
en-aut-sei=Sasano
en-aut-mei=Shizuka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KatsuharaMaki
en-aut-sei=Katsuhara
en-aut-mei=Maki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MoriIzumi C.
en-aut-sei=Mori
en-aut-mei=Izumi C.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=2
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=3
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=4
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=5
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=6
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=
en-keyword=Arabidopsis thaliana
kn-keyword=Arabidopsis thaliana
en-keyword=CO2 transport
kn-keyword=CO2 transport
en-keyword=monomeric pore
kn-keyword=monomeric pore
en-keyword=PIP2 aquaporin
kn-keyword=PIP2 aquaporin
en-keyword=Xenopus laevis
kn-keyword=Xenopus laevis
END

start-ver=1.4
cd-journal=joma
no-vol=262
cd-vols=
no-issue=2
article-no=
start-page=385
end-page=395
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20241023
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Analysis of the effect of permeant solutes on the hydraulic resistance of the plasma membrane in cells of Chara corallina
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In the cells of Chara corallina, permeant monohydric alcohols including methanol, ethanol and 1-propanol increased the hydraulic resistance of the membrane (Lpm−1). We found that the relative value of the hydraulic resistance (rLpm−1) was linearly dependent on the concentration (Cs) of the alcohol. The relationship is expressed in the equation: rLpm−1 = ρmCs + 1, where ρm is the hydraulic resistance modifier coefficient of the membrane. Ye et al. (2004) showed that membrane-permeant glycol ethers also increased Lp−1. We used their data to estimate Lpm−1 and rLpm−1. The values of rLpm−1 fit the above relation we found for alcohols. When we plotted the ρm values of all the permeant alcohols and glycol ethers against their molecular weights (MW), we obtained a linear curve with a slope of 0.014 M−1/MW and with a correlation coefficient of 0.99. We analyzed the influence of the permeant solutes on the relative hydraulic resistance of the membrane (rLpm−1) as a function of the external (π0) and internal (πi) osmotic pressures. The analysis showed that the hydraulic resistance modifier coefficients (ρm) were linearly related to the MW of the permeant solutes with a slope of 0.012 M−1/MW and with a correlation coefficient of 0.84. The linear relationship between the effects of permeating solutes on the hydraulic resistance modifier coefficient (ρm) and the MW can be explained in terms of the effect of the effective osmotic pressure on the hydraulic conductivity of water channels. The result of the analysis suggests that the osmotic pressure and not the size of the permeant solute as proposed by (Ye et al., J Exp Bot 55:449–461, 2004) is the decisive factor in a solute’s influence on hydraulic conductivity. Thus, characean water channels (aquaporins) respond to permeant solutes with essentially the same mechanism as to impermeant solutes.
en-copyright=
kn-copyright=

en-aut-name=TazawaMasashi
en-aut-sei=Tazawa
en-aut-mei=Masashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=WayneRandy
en-aut-sei=Wayne
en-aut-mei=Randy
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KatsuharaMaki
en-aut-sei=Katsuhara
en-aut-mei=Maki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=Yoshida Biological Laboratory
kn-affil=

affil-num=2
en-affil=Laboratory of Natural Philosophy, Plant Biology Section, Cornell University
kn-affil=

affil-num=3
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=
en-keyword=Chara corallina
kn-keyword=Chara corallina
en-keyword=Effective osmotic pressure
kn-keyword=Effective osmotic pressure
en-keyword=Hydraulic resistance
kn-keyword=Hydraulic resistance
en-keyword=Plasma membrane
kn-keyword=Plasma membrane
en-keyword=Reflection coefficient
kn-keyword=Reflection coefficient
END

start-ver=1.4
cd-journal=joma
no-vol=227
cd-vols=
no-issue=
article-no=
start-page=110168
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=202510
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The hidden cation-selective pore in ion-conducting aquaporin OsPIP2;4 from rice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Ion-conducting aquaporins (icAQPs) transport ions as well as water. Although the molecular mechanism of how AQPs establish selective permeability for water molecules is well understood, the ion-transporting mechanism in icAQPs has not yet been fully elucidated. In this study, we investigated the molecular mechanism of cation transport in OsPIP2;4, an icAQP in rice, by homology modeling and the electrophysiological analysis using Xenopus laevis oocytes. Water and ion transport assays using OsPIP2;4 T227M and G278K mutants strongly suggested that water- and cation-transporting pathways are independent of each other. Data from amino acid substitutions V54I and A143G in OsPIP2;4 led to the identification of a novel hidden pathway for cation transport located on the side surfaces of the tetramer channel, where two protomers are in contact, which is distinct from conventional monomeric pores and the tetrameric central pore in AQPs. Moreover, the present results provide the possibility that this hypothetical hidden pore also functions in the barley icAQP HvPIP2;8. The overall structure of this novel pathway appears to differ from the structure of general cation channels. However, the arrangement of hydrophilic amino acids at the entrance of the pathway of OsPIP2;4 was found to be comparable to that of some cation channels, which implies that the molecular mechanism of dehydration of hydrated ions might resemble that of the channels. Although direct structural evidence is needed to confirm the proposed pathway, the present study can be a stepping stone toward unraveling the mechanism of dual water and ion transport through icAQPs in plants.
en-copyright=
kn-copyright=

en-aut-name=OnoShuntaro
en-aut-sei=Ono
en-aut-mei=Shuntaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TranSen Thi Huong
en-aut-sei=Tran
en-aut-mei=Sen Thi Huong
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SaitohYasunori
en-aut-sei=Saitoh
en-aut-mei=Yasunori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=UtsugiShigeko
en-aut-sei=Utsugi
en-aut-mei=Shigeko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=HorieTomoaki
en-aut-sei=Horie
en-aut-mei=Tomoaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KatsuharaMaki
en-aut-sei=Katsuhara
en-aut-mei=Maki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=2
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=3
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=

affil-num=4
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=5
en-affil=Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
kn-affil=

affil-num=6
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=
en-keyword=Rice
kn-keyword=Rice
en-keyword=Barley
kn-keyword=Barley
en-keyword=Ion transport
kn-keyword=Ion transport
en-keyword=Ion-conducting aquaporin (icAQP)
kn-keyword=Ion-conducting aquaporin (icAQP)
en-keyword=Plasma membrane intrinsic protein (PIP)
kn-keyword=Plasma membrane intrinsic protein (PIP)
END

start-ver=1.4
cd-journal=joma
no-vol=10
cd-vols=
no-issue=10
article-no=
start-page=2006
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210924
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Identification and Characterization of Rice OsHKT1;3 Variants
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In rice, the high-affinity K+ transporter, OsHKT1;3, functions as a Na+-selective transporter. mRNA variants of OsHKT1;3 have been reported previously, but their functions remain unknown. In this study, five OsHKT1;3 variants (V1-V5) were identified from japonica rice (Nipponbare) in addition to OsHKT1;3_FL. Absolute quantification qPCR analyses revealed that the transcript level of OsHKT1;3_FL was significantly higher than other variants in both the roots and shoots. Expression levels of OsHKT1;3_FL, and some variants, increased after 24 h of salt stress. Two electrode voltage clamp experiments in a heterologous expression system using Xenopus laevis oocytes revealed that oocytes expressing OsHKT1;3_FL and all of its variants exhibited smaller Na+ currents. The presented data, together with previous data, provide insights to understanding how OsHKT family members are involved in the mechanisms of ion homeostasis and salt tolerance in rice.</p>
en-copyright=
kn-copyright=

en-aut-name=ImranShahin
en-aut-sei=Imran
en-aut-mei=Shahin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TsuchiyaYoshiyuki
en-aut-sei=Tsuchiya
en-aut-mei=Yoshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TranSen Thi Huong
en-aut-sei=Tran
en-aut-mei=Sen Thi Huong
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KatsuharaMaki
en-aut-sei=Katsuhara
en-aut-mei=Maki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=2
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=3
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=4
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=
en-keyword=Na+ transport
kn-keyword=Na+ transport
en-keyword=rice
kn-keyword=rice
en-keyword=OsHKT1
kn-keyword=OsHKT1
en-keyword=3
kn-keyword=3
en-keyword=mRNA variants
kn-keyword=mRNA variants
en-keyword=TEVC
kn-keyword=TEVC
END

start-ver=1.4
cd-journal=joma
no-vol=21
cd-vols=
no-issue=19
article-no=
start-page=7135
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200927
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A Survey of Barley PIP Aquaporin Ionic Conductance Reveals Ca2+-Sensitive HvPIP2;8 Na+ and K+ Conductance
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Some plasma membrane intrinsic protein (PIP) aquaporins can facilitate ion transport. Here we report that one of the 12 barley PIPs (PIP1 and PIP2) tested, HvPIP2;8, facilitated cation transport when expressed in Xenopus laevis oocytes. HvPIP2;8-associated ion currents were detected with Na+ and K+, but not Cs+, Rb+, or Li+, and was inhibited by Ba2+, Ca2+, and Cd2+ and to a lesser extent Mg2+, which also interacted with Ca2+. Currents were reduced in the presence of K+, Cs+, Rb+, or Li+ relative to Na+ alone. Five HvPIP1 isoforms co-expressed with HvPIP2;8 inhibited the ion conductance relative to HvPIP2;8 alone but HvPIP1;3 and HvPIP1;4 with HvPIP2;8 maintained the ion conductance at a lower level. HvPIP2;8 water permeability was similar to that of a C-terminal phosphorylation mimic mutant HvPIP2;8 S285D, but HvPIP2;8 S285D showed a negative linear correlation between water permeability and ion conductance that was modified by a kinase inhibitor treatment. HvPIP2;8 transcript abundance increased in barley shoot tissues following salt treatments in a salt-tolerant cultivar Haruna-Nijo, but not in salt-sensitive I743. There is potential for HvPIP2;8 to be involved in barley salt-stress responses, and HvPIP2;8 could facilitate both water and Na+/K+ transport activity, depending on the phosphorylation status.
en-copyright=
kn-copyright=

en-aut-name=Sen Thi HuongTran
en-aut-sei=Sen Thi Huong
en-aut-mei=Tran
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HorieTomoaki
en-aut-sei=Horie
en-aut-mei=Tomoaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ImranShahin
en-aut-sei=Imran
en-aut-mei=Shahin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=QiuJiaen
en-aut-sei=Qiu
en-aut-mei=Jiaen
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=McGaugheySamantha
en-aut-sei=McGaughey
en-aut-mei=Samantha
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=ByrtCaitlin S.
en-aut-sei=Byrt
en-aut-mei=Caitlin S.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TyermanStephen D.
en-aut-sei=Tyerman
en-aut-mei=Stephen D.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KatsuharaMaki
en-aut-sei=Katsuhara
en-aut-mei=Maki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=2
en-affil=Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
kn-affil=

affil-num=3
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=4
en-affil=Australian Research Council Centre of Excellence in Plant Energy Biology, Waite Research Institute and School of Agriculture, Food and Wine, The University of Adelaide
kn-affil=

affil-num=5
en-affil=Research School of Biology, Australian National University
kn-affil=

affil-num=6
en-affil=Australian Research Council Centre of Excellence in Plant Energy Biology, Waite Research Institute and School of Agriculture, Food and Wine, The University of Adelaide
kn-affil=

affil-num=7
en-affil=Australian Research Council Centre of Excellence in Plant Energy Biology, Waite Research Institute and School of Agriculture, Food and Wine, The University of Adelaide
kn-affil=

affil-num=8
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=
en-keyword=aquaporins
kn-keyword=aquaporins
en-keyword=barley
kn-keyword=barley
en-keyword=ion transport
kn-keyword=ion transport
en-keyword=oocytes
kn-keyword=oocytes
en-keyword=plasma membrane intrinsic proteins (PIPs)
kn-keyword=plasma membrane intrinsic proteins (PIPs)
END

start-ver=1.4
cd-journal=joma
no-vol=9
cd-vols=
no-issue=1
article-no=
start-page=16
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20191221
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Expression and Ion Transport Activity of Rice OsHKT1;1 Variants
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=OsHKT1;1 in rice, belongs to the high-affinity K+ Transporter family, has been found to be involved in salt tolerance. OsHKT1;1 in japonica rice (Nipponbare) produces mRNA variants, but their functions remain elusive. In salt tolerant rice, Pokkali, eight OsHKT1;1 variants (V1-V8) were identified in addition to the full-length OsHKT1;1 (FL) cDNA. Absolute quantification by qPCR revealed that accumulation of OsHKT1;1-FL mRNA is minor in contrast to that of OsHKT1;1-V1, -V2, -V4, and -V7 mRNAs, all of which are predominant in shoots, while only V1 and V7 mRNAs are predominant in roots. Two electrode voltage clamp (TEVC) experiments using Xenopus laevis oocytes revealed that oocytes-expressing OsHKT1;1-FL from Pokkali exhibited inward-rectified currents in the presence of 96 mM Na+ as reported previously. Further TEVC analyses indicated that six of eight OsHKT1;1 variants elicited currents in a Na+ or a K+ bath solution. OsHKT1;1-V6 exhibited a similar inward rectification to the FL protein. Contrastingly, however, the rests mediated bidirectional currents in both Na+ and K+ bath solutions. These data suggest possibilities that novel mechanisms regulating the transport activity of OsHKT1;1 might exist, and that OsHKT1;1 variants might also carry out distinct physiological roles either independently or in combination with OsHKT1;1-FL.
en-copyright=
kn-copyright=

en-aut-name=ImranShahin
en-aut-sei=Imran
en-aut-mei=Shahin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HorieTomoaki
en-aut-sei=Horie
en-aut-mei=Tomoaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KatsuharaMaki
en-aut-sei=Katsuhara
en-aut-mei=Maki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=2
en-affil=Division of Applied Biology, Faculty of the Textile Science and Technology, Shinshu University
kn-affil=

affil-num=3
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=
en-keyword=HKT
kn-keyword=HKT
en-keyword=rice
kn-keyword=rice
en-keyword=salt tolerance
kn-keyword=salt tolerance
en-keyword=Na+ transport
kn-keyword=Na+ transport
en-keyword=K+ transport
kn-keyword=K+ transport
en-keyword=mRNA variants
kn-keyword=mRNA variants
END

start-ver=1.4
cd-journal=joma
no-vol=42
cd-vols=
no-issue=2
article-no=
start-page=437
end-page=447
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=20180716
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The mechanism of SO2 -induced stomatal closure differs from O3 and CO2 responses and is mediated by nonapoptotic cell death in guard cells.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Plants closing stomata in the presence of harmful gases is believed to be a stress avoidance mechanism. SO2 , one of the major airborne pollutants, has long been reported to induce stomatal closure, yet the mechanism remains unknown. Little is known about the stomatal response to airborne pollutants besides O3 . SLOW ANION CHANNEL-ASSOCIATED 1 (SLAC1) and OPEN STOMATA 1 (OST1) were identified as genes mediating O3 -induced closure. SLAC1 and OST1 are also known to mediate stomatal closure in response to CO2 , together with RESPIRATORY BURST OXIDASE HOMOLOGs (RBOHs). The overlaying roles of these genes in response to O3 and CO2 suggested that plants share their molecular regulators for airborne stimuli. Here, we investigated and compared stomatal closure event induced by a wide concentration range of SO2 in Arabidopsis through molecular genetic approaches. O3 - and CO2 -insensitive stomata mutants did not show significant differences from the wild type in stomatal sensitivity, guard cell viability, and chlorophyll content revealing that SO2 -induced closure is not regulated by the same molecular mechanisms as for O3 and CO2 . Nonapoptotic cell death is shown as the reason for SO2 -induced closure, which proposed the closure as a physicochemical process resulted from SO2 distress, instead of a biological protection mechanism.
en-copyright=
kn-copyright=

en-aut-name=Ooi Lia
en-aut-sei=Ooi 
en-aut-mei=Lia
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MatsuuraTakakazu
en-aut-sei=Matsuura
en-aut-mei=Takakazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MunemasaShintaro
en-aut-sei=Munemasa
en-aut-mei=Shintaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MurataYoshiyuki
en-aut-sei=Murata
en-aut-mei=Yoshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KatsuharaMaki
en-aut-sei=Katsuhara
en-aut-mei=Maki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HirayamaTakashi
en-aut-sei=Hirayama
en-aut-mei=Takashi
kn-aut-name=平山隆志
kn-aut-sei=平山
kn-aut-mei=隆志
aut-affil-num=6
ORCID=

en-aut-name=MoriIzumi C.
en-aut-sei=Mori
en-aut-mei=Izumi C.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil= Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=2
en-affil= Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=4
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=5
en-affil= Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=6
en-affil= Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=7
en-affil= Institute of Plant Science and Resources, Okayama University
kn-affil=
en-keyword=airborne pollutants
kn-keyword=airborne pollutants
en-keyword=nonapoptotic cell death
kn-keyword=nonapoptotic cell death
en-keyword=stomatal closure
kn-keyword=stomatal closure
en-keyword=sulfur dioxide
kn-keyword=sulfur dioxide
END

start-ver=1.4
cd-journal=joma
no-vol=31
cd-vols=
no-issue=
article-no=
start-page=21
end-page=25
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=200912
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Cooling effect on buildings by the roof greening at Research Institute for Bioresources, Okayama University
kn-title=岡山大学資源生物科学研究所における屋上緑化による建物冷却効果
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Roof greening is known to be environmentally friendly technology. Recently developed new roof greening systems, such as the thin-layer/Excel soil© system and the wetland type greening system, were tested at the roof top of buildings of Research Institute for Bioresources, Okayama University. After a multi-year test, these new systems have been established during high-temperature and less-rainfall summer seasons in the south Okayama region. Data indicated that roof greening effectively reduced the temperature of the concrete surface (more than 10°C). The room temperature under the green roof was also reduced both in a stock room (up to 6°C) and in an office room (about 2°C). We also provided the estimation indicating that this roof greening is useful for the decrease in CO(2) emission through the reduction of the electric power for air-conditioning in the summer.
en-copyright=
kn-copyright=

en-aut-name=KatsuharaMaki
en-aut-sei=Katsuhara
en-aut-mei=Maki
kn-aut-name=且原真木
kn-aut-sei=且原
kn-aut-mei=真木
aut-affil-num=1
ORCID=

en-aut-name=TanakamaruShigemi
en-aut-sei=Tanakamaru
en-aut-mei=Shigemi
kn-aut-name=田中丸重美
kn-aut-sei=田中丸
kn-aut-mei=重美
aut-affil-num=2
ORCID=

en-aut-name=MoriIzumi C.
en-aut-sei=Mori
en-aut-mei=Izumi C.
kn-aut-name=森泉
kn-aut-sei=森
kn-aut-mei=泉
aut-affil-num=3
ORCID=

en-aut-name=TaniAkio
en-aut-sei=Tani
en-aut-mei=Akio
kn-aut-name=谷明生
kn-aut-sei=谷
kn-aut-mei=明生
aut-affil-num=4
ORCID=

en-aut-name=UtsugiShigeko
en-aut-sei=Utsugi
en-aut-mei=Shigeko
kn-aut-name=宇都木繁子
kn-aut-sei=宇都木
kn-aut-mei=繁子
aut-affil-num=5
ORCID=

en-aut-name=EnomotoTakashi
en-aut-sei=Enomoto
en-aut-mei=Takashi
kn-aut-name=榎本敬
kn-aut-sei=榎本
kn-aut-mei=敬
aut-affil-num=6
ORCID=

en-aut-name=MaitaniToshihiko
en-aut-sei=Maitani
en-aut-mei=Toshihiko
kn-aut-name=米谷俊彦
kn-aut-sei=米谷
kn-aut-mei=俊彦
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学資源生物科学研究所

affil-num=2
en-affil=
kn-affil=岡山大学資源生物科学研究所

affil-num=3
en-affil=
kn-affil=岡山大学資源生物科学研究所

affil-num=4
en-affil=
kn-affil=岡山大学資源生物科学研究所

affil-num=5
en-affil=
kn-affil=岡山大学資源生物科学研究所

affil-num=6
en-affil=
kn-affil=岡山大学資源生物科学研究所

affil-num=7
en-affil=
kn-affil=岡山大学資源生物科学研究所
en-keyword=Roof greening
kn-keyword=Roof greening
en-keyword=wetland type greening
kn-keyword=wetland type greening
en-keyword=thin-Iayer/ Excel soil© system
kn-keyword=thin-Iayer/ Excel soil© system
en-keyword=cooling effect
kn-keyword=cooling effect
END

start-ver=1.4
cd-journal=joma
no-vol=456
cd-vols=
no-issue=4
article-no=
start-page=687
end-page=691
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=20080626
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Barley plasma membrane intrinsic proteins (PIP aquaporins) as water and CO2 transporters
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>We identified barley aquaporins and demonstrated that one, HvPIP2;1, transports water and CO2. Regarding water homeostasis in plants, regulations of aquaporin expression were observed in many plants under several environmental stresses. Under salt stress, a number of plasma membrane-type aquaporins were down-regulated, which can prevent continuous dehydration resulting in cell death. The leaves of transgenic rice plants that expressed the largest amount of HvPIP2;1 showed a 40% increase in internal CO2 conductance compared with leaves of wild-type rice plants. The rate of CO2 assimilation also increased in the transgenic plants. The goal of our plant aquaporin research is to determine the key aquaporin species responsible for water and CO2 transport, and to improve plant water relations, stress tolerance, CO2 uptake or assimilation, and plant productivity via molecular breeding of aquaporins.</p>

en-copyright=
kn-copyright=

en-aut-name=KatsuharaMaki
en-aut-sei=Katsuhara
en-aut-mei=Maki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HanbaYuko T.
en-aut-sei=Hanba
en-aut-mei=Yuko T.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=Research Institute for Bioresources, Okayama University

affil-num=2
en-affil=
kn-affil=Center for Bioresource Field Science, Kyoto Institute of Technology
en-keyword=barley
kn-keyword=barley
en-keyword=CO2
kn-keyword=CO2
en-keyword=plant aquaporins
kn-keyword=plant aquaporins
en-keyword=salt stress
kn-keyword=salt stress
en-keyword=water transport
kn-keyword=water transport
END

start-ver=1.4
cd-journal=joma
no-vol=3
cd-vols=
no-issue=2
article-no=
start-page=145
end-page=149
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1995
dt-pub=1995
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Cloning of PCR-Products Encoding Potassium Channel Proteins from Mesembryanthemum crystallinum
kn-title=Mesembryanthemum crystallinum(ice plant)におけるカリウムチャンネルをコードするPCR産物のクローニング
en-subtitle=
kn-subtitle=
en-abstract=耐塩性、耐乾性の極めて高い Mesembryanthemum crystallinum からPCR法を用いてカリウムチャンネル遺伝子断片を得た。2つのクローンが独立に得られたが、互いによく似ていて、シロイヌナズナのカリウムチャンネルとは67から88％の相同性を示した。サザンハイブリダイゼーションの結果から、今回得られた遺伝子はシングルコピーであり、またカリウムチャンネル遺伝子ファミリーが存在する可能性が示唆された。
kn-abstract=Gene fragments of potassium channels were cloned from Mesembryanthemum crystallinum by using RT-PCR (reverse transcription-polymerase chain reaction). The two fragments were isolated independently and showed high similarity with each other. About 80% identity was found between the two fragments and potassium-channel genes of Arabidopsis. Southern hybridization indicated that the potassium channel gene may be a single copy gene or that a small gene family of potassium channels exists.
en-copyright=
kn-copyright=

en-aut-name=KatsuharaMaki
en-aut-sei=Katsuhara
en-aut-mei=Maki
kn-aut-name=且原真木
kn-aut-sei=且原
kn-aut-mei=真木
aut-affil-num=1
ORCID=

en-aut-name=BohnertHans J.
en-aut-sei=Bohnert
en-aut-mei=Hans J.
kn-aut-name=BohnertHans J.
kn-aut-sei=Bohnert
kn-aut-mei=Hans J.
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学
en-keyword=Mesembryanthemum crystallinum
kn-keyword=Mesembryanthemum crystallinum
en-keyword=Potassium channel
kn-keyword=Potassium channel
en-keyword=RT-PCR
kn-keyword=RT-PCR
END