ID | 32935 |
フルテキストURL | |
著者 |
Hobara, Narumi
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Goda, Mitsuhiro
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Yoshida, Namika
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kitamura, Yoshihisa
Department of Pharmaceutical Care and Health Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
ORCID
Kaken ID
publons
researchmap
Mio, Mitsunobu
Shujitsu University School of Pharmacy
|
抄録 | The present study was designed to investigate involvement of angiotensin (Ang) II type 2 receptors (AT2 receptors) in restoration of perivascular nerve innervation injured by topical phenol treatment. Male Wistar rats underwent in vivo topical application of 10% phenol around the superior mesenteric artery. After phenol treatment, animals were subjected to immunohistochemistry of the third branch of small arteries, Western blot analysis of AT2 receptor protein expression in dorsal root ganglia (DRG) and studies of mesenteric neurogenic vasoresponsiveness. Ang II (750 ng/kg/day), nerve growth factor (NGF; 20 μg/kg/day) and PD123,319 (AT2 receptor antagonist; 10 mg/kg/day) were intraperitoneally administered for 7 days using osmotic mini-pumps immediately after topical phenol treatment. Losartan (AT1 receptor antagonist) was administered in drinking water (0.025%). Phenol treatment markedly reduced densities of both calcitonin gene-related peptide (CGRP)-like immunoreactivity (LI)- and neuropeptide Y (NPY)-LI-containing fibers. NGF restored densities of both nerve fibers to the Sham control level. Coadministration of Ang II and losartan significantly increased the density of CGRP-LI-fibers but not NPY-LI-fibers compared with saline control. The increase of the density of CGRP-LI-fibers by coadministration of Ang II and losartan was suppressed by adding PD123,319. Coadministration of Ang II and losartan ameliorated reduction of CGRP nerve-mediated vasodilation of perfused mesenteric arteries caused by phenol treatment. The AT2 receptor protein expression detected in DRG was markedly increased by NGF. These results suggest that selective stimulation of AT2 receptors by Ang II facilitates reinnervation of mesenteric perivascular CGRP-containing nerves injured by topical phenol application in the rat. |
キーワード | Angiotensin II type 2 receptors
Phenol-induced perivascular nerve injury
Calcitonin gene-related peptide-containing nerves
Neuropeptide Y-containing nerves
Neurotrophic
Rat mesenteric artery
|
備考 | Published with permission from the copyright holder.
This is a author's copy,as published in NEUROSCIENCE , 2007, volume 150, issue 3, pp730-741. Publisher URL: http://dx.doi.org/10.1016/j.neuroscience.2007.09.026 Direct access to Thomson Web of Science record Copyright © 2007 by the National Academy of Sciences |
発行日 | 2008-05-28
|
出版物タイトル |
Neuroscience
|
巻 | 150巻
|
号 | 3号
|
開始ページ | 730
|
終了ページ | 741
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
査読 |
有り
|
DOI | |
Web of Science KeyUT | |
Submission Path | neuroscience/2
|