start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=1
article-no=
start-page=13
end-page=24
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=201602
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The Function of ƒÀ2-glycoprotein I in Angiogenesis and Its in Vivo Distribution in Tumor Xenografts
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Intact ƒÀ2-glycoprotein I (iƒÀ2GPI) is a glycoprotein that regulates coagulation and fibrinolysis. Nicked ƒÀ2GPI (nƒÀ2GPI) possesses an angiogenic property at a relatively low concentration, and an antiangiogenic property at a high concentration. Here we investigated the functions of ƒÀi 2GPI and nƒÀ2GPI in vascular endothelial growth factor (VEGF)-A-induced endothelial cell proliferation and tube formation. We used noninvasive PET imaging to analyze the in vivo distribution of intravenously injected ƒÀ2GPI variants in tumor lesions in mice. iƒÀ2GPI was incubated with plasmin to obtain nƒÀ2GPI, and its N-terminal sequence was analyzed. nƒÀ2GPI had at least one other cleavage site upstream of the ƒÀ2GPI?s domain V, whereas the former plasmin-cleavage site locates between K317 and T318. Both of intact and nicked ƒÀ2GPI significantly inhibited the VEGF-A-induced cell proliferation and the tube formation of human umbilical vein endothelial cells (HUVECs). PET imaging visualized considerably distributed intensities of all tested ƒÀ2GPI variants in tumor lesions of pancreatic tumor cell-xenografts. These results indicate that ƒÀ2GPI may be physiologically and pathophysiologically important in the regulation of not only coagulation and fibrinolysis, but also angiogenesis.
en-copyright=
kn-copyright=

en-aut-name=Arum Tri Wahyuningsih
en-aut-sei=Arum Tri Wahyuningsih
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ShenLianhua
en-aut-sei=Shen
en-aut-mei=Lianhua
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KobayashiKazuko
en-aut-sei=Kobayashi
en-aut-mei=Kazuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SasakiTakanori
en-aut-sei=Sasaki
en-aut-mei=Takanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TakenakaFumiaki
en-aut-sei=Takenaka
en-aut-mei=Fumiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HanadaTakahisa
en-aut-sei=Hanada
en-aut-mei=Takahisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=AkehiMasaru
en-aut-sei=Akehi
en-aut-mei=Masaru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=AkahoshiAkiya
en-aut-sei=Akahoshi
en-aut-mei=Akiya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=OzekiEiichi
en-aut-sei=Ozeki
en-aut-mei=Eiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=AndoEiji
en-aut-sei=Ando
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Cell Chemistry , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=2
en-affil=
kn-affil=Collaborative Research Center (OMIC), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=3
en-affil=
kn-affil=Collaborative Research Center (OMIC), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=4
en-affil=
kn-affil=Collaborative Research Center (OMIC), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=5
en-affil=
kn-affil=Collaborative Research Center (OMIC), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=6
en-affil=
kn-affil=Collaborative Research Center (OMIC), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=7
en-affil=
kn-affil=Collaborative Research Center (OMIC), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=8
en-affil=
kn-affil=Collaborative Research Center (OMIC), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=9
en-affil=
kn-affil=Technology Research Laboratory, Shimadzu Corporation

affil-num=10
en-affil=
kn-affil=Life Science Business Department, Shimadzu Corporation

affil-num=11
en-affil=
kn-affil=Department of Cell Chemistry , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
en-keyword=ƒÀ2-glycoprotein I (ƒÀ2GPI)
kn-keyword=ƒÀ2-glycoprotein I (ƒÀ2GPI)
en-keyword=angiogenesis
kn-keyword=angiogenesis
en-keyword=vascular endothelial growth factor-A (VEGF-A)
kn-keyword=vascular endothelial growth factor-A (VEGF-A)
en-keyword=positron emission tomography (PET) imaging
kn-keyword=positron emission tomography (PET) imaging
END