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ID 49561
フルテキストURL
著者
Tian, FengFeng Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Deguchi, Kentaro Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Yamashita, Toru Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Ohta, Yasuyuki Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Morimoto, Nobutoshi Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Shang, Jingwei Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Zhang, Xuemei Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Liu, Ning Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Ikeda, Yoshio Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Matsuura, Tohru Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Abe, Koji Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci Kaken ID
抄録
Recent studies have suggested that autophagy is involved in a neural death pathway following cerebral ischemia. In vivo detection of autophagy could be important for evaluating ischemic neural cell damage for human stroke patients. Using novel green fluorescent protein (GFP)-fused microtubule-associated protein 1 light chain 3 (LC3) transgenic (Tg) mice, in vivo imaging of autophagy was performed at 1, 3 and 6 d after 60 min transient middle cerebral artery occlusion (tMCAO). Ex vivo imaging of autophagy, testing of the autophagy inhibitor 3-methyladenine (3-MA), estern blot analysis, immunohistochemistry, terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) and fluorescent analyses were performed on brain sections following tMCAO. In vivo fluorescent signals were detected above the ischemic hemisphere through the skull bone at 1, 3 and 6 d after tMCAO, with a peak at 1 d. Similar results were obtained with ex vivo fluorescence imaging. western blot analysis revealed maximum LC3-I and LC3-II expression at 1 d after tMCAO and fluorescence immunohistochemistry demonstrated that GFP-LC3-positive cells were primarily neuronal, not astroglial or microglial, cells. The number of GFP-LC3/TUNEL double-positive cells was greater in the peri-ischemic area than in the core. These results provided evidence of in vivo autophagy detection, with a peak at 1 d, in a live animal model following cerebral ischemia. This novel technique could be valuable for monitoring autophagic processes in vivo in live stroke patients, as well as for clarifying the detailed role of autophagy in the ischemic brain, as well as in other neurological diseases.
キーワード
autophagy
apoptosis
GFP-LC3 Tg mice
in vivo imaging
tMCAO
発行日
2010-11-16
出版物タイトル
Autophagy
6巻
8号
開始ページ
1107
終了ページ
1114
ISSN
1554-8627
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
author
PubMed ID
DOI
Web of Sience KeyUT
オフィシャル URL
http://doi.org/10.4161/auto.6.8.13427
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/49128