start-ver=1.4
cd-journal=joma
no-vol=55
cd-vols=
no-issue=3
article-no=
start-page=161
end-page=165
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2001
dt-pub=200106
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Nephrin is an important component of the barrier system in the testis.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>Nephrin, a gene product of the congenital nephrotic syndrome of the Finnish type (NPHS1), is a 1242-residue putative transmembrane protein of the immunoglobulin family of cell adhesion molecules. The expression of this gene is localized in rat and human glomerular epithelial cells. Here we report the expression of nephrin in various tissues other than the kidneys in mice. The expression of nephrin mRNA in various tissues of mice, including the kidneys, testes, spleen, thymus and brain, were first investigated by the RT-PCR method, and it was shown that a high level of nephrin mRNA could be detected in the testes of mice 1-6 weeks old. In situ hybridization revealed the expression of the nephrin gene in the Sertoli cells. Additionally, immunofluorescent staining studies indicated that nephrin was colocalized with anchoring protein ZO-1 in the mouse testis. From these results, it is inferred that nephrin is an important component of the barrier system in testes.</p>

en-copyright=
kn-copyright=

en-aut-name=LiuLi
en-aut-sei=Liu
en-aut-mei=Li
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=AyaKunihiko
en-aut-sei=Aya
en-aut-mei=Kunihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TanakaHiroyuki
en-aut-sei=Tanaka
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=ShimizuJunya
en-aut-sei=Shimizu
en-aut-mei=Junya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=ItoShigeru
en-aut-sei=Ito
en-aut-mei=Shigeru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=SeinoYoshiki
en-aut-sei=Seino
en-aut-mei=Yoshiki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama University

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Okayama University
en-keyword=nephrin
kn-keyword=nephrin
en-keyword=Sertoli cells
kn-keyword=Sertoli cells
en-keyword=glomerular epithelial cells
kn-keyword=glomerular epithelial cells
en-keyword=blood barrier
kn-keyword=blood barrier
END

start-ver=1.4
cd-journal=joma
no-vol=109
cd-vols=
no-issue=7-12
article-no=
start-page=133
end-page=140
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1997
dt-pub=19971225
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Expression of parathyroid-related peptide mRNA in the kidney
kn-title=腎臓における副甲状腺ホルモン関連ペプチドの局在の検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=PTHrP mRNA was quantified by RT-PCR and the localization of PTHrP mRNA was determined by in situ hybridization in kidney. During development, the expression of PTHrP mRNA is much higher before 2 weeks of age while kidney is developing. In E16 fetal mice, PTHrP mRNA was strongly expressed in the collecting ducts, urothelium of the pelvis, tubles, the glomeruli and immature elements in the cortex of the developing kidney (nephrogenic zone). within 0-day-old-mice, it was dominantly expressed in collecting ducts, the urothelium of the pelvis and nephrogenic zone. These findings suggest that PTHrP plays a role in kidney development as an autocrine/ paracrine factor.
en-copyright=
kn-copyright=

en-aut-name=AyaKunihiko
en-aut-sei=Aya
en-aut-mei=Kunihiko
kn-aut-name=綾邦彦
kn-aut-sei=綾
kn-aut-mei=邦彦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部小児科学講座
en-keyword=副甲状腺ホルモン関連ペプチド（PTHrP)
kn-keyword=副甲状腺ホルモン関連ペプチド（PTHrP)
en-keyword=腎臓
kn-keyword=腎臓
en-keyword=発達
kn-keyword=発達
en-keyword=in situ hybridization
kn-keyword=in situ hybridization
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1997
dt-pub=19970930
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=腎臓における副甲状腺ホルモン関連ペプチドの局在の検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=綾邦彦
kn-aut-sei=綾
kn-aut-mei=邦彦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
END