In order to elucidate the mechanism of latent infection of herpes simplex virus (HSV), reactivatable latency of three avirulent strains (SKO-1B, -GCr Miyama, SKa) of HSV type 1 was comparatively examined in a mouse latency model. The SKO-1B strain showed high rate of virus reactivation from explanted trigeminal ganglia without n-butyrate enhancement, while the other two strains showed a very low rate of virus reactivation in the absence of n-butyrate. In the presence of n-butyrate, however, the rate of the -GCr Miyama strain jumped to a comparable level with that of SKO-1B, although the rate of SKa remained at a low level. A more precise follow-up experiment changing the virus dose highlighted the difference of the ability to reactivate from the latent state between SKO-1B and -GCr Miyama. Virus titer in trigeminal ganglia during acute phase, infectivity to cell lines of neural origin, and susceptibility to acyclovir and phosphonoacetate were assayed to know the reasons for the variation in the ability of reactivatable latency among these strains. It was concluded that the reduced infectivity to neural cells, and limited ability of reactivatable latency shown by the SKa strain could mainly be attributed to the deficiency of thymidine kinase activity.</P>
en-copyright= kn-copyright= en-aut-name=AraoYujiro en-aut-sei=Arao en-aut-mei=Yujiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HatanoAtsushi en-aut-sei=Hatano en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamadaMasao en-aut-sei=Yamada en-aut-mei=Masao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=UnoFumio en-aut-sei=Uno en-aut-mei=Fumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NiiShiro en-aut-sei=Nii en-aut-mei=Shiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=The University of Tokushima affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Niimi Women's College affil-num=5 en-affil= kn-affil=Okayama University en-keyword=herpes simplex virus type 1 kn-keyword=herpes simplex virus type 1 en-keyword=neurovirulence kn-keyword=neurovirulence en-keyword=latency kn-keyword=latency en-keyword=reactivation kn-keyword=reactivation en-keyword=n-butyrate kn-keyword=n-butyrate END start-ver=1.4 cd-journal=joma no-vol=45 cd-vols= no-issue=2 article-no= start-page=117 end-page=121 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=199104 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Neurovirulent strains of herpes simplex virus type 1 are not necessarily competent for reactivatable latency. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Ability of two neurovirulent strains (F and +GC (LPV) Miyama) of herpes simplex virus type 1 (HSV-1) to establish and maintain reactivatable latency in trigeminal ganglia (TG) was compared after intranasal inoculation of mice. The +GC (LPV) Miyama strain showed a very low rate of virus reactivation in explant cultures of TG, while the F strain showed a high rate of reactivation. These data indicate that neurovirulent strains of HSV-1 are not always competent for reactivatable latency, although most virulent strains of HSV-1 thus far reported were competent for reactivatable latency.</P>
en-copyright= kn-copyright= en-aut-name=AraoYujiro en-aut-sei=Arao en-aut-mei=Yujiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HatanoAtsushi en-aut-sei=Hatano en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamadaMasao en-aut-sei=Yamada en-aut-mei=Masao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=UnoFumio en-aut-sei=Uno en-aut-mei=Fumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NiiShiro en-aut-sei=Nii en-aut-mei=Shiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=The University of Tokushima affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Niimi Woman's College affil-num=5 en-affil= kn-affil=Okayama University en-keyword=herpes simplex virus type 1 kn-keyword=herpes simplex virus type 1 en-keyword=neurovirulence kn-keyword=neurovirulence en-keyword=latency kn-keyword=latency en-keyword=reactivation kn-keyword=reactivation END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=19901231 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=単純ヘルペスウイルス1型潜伏感染の研究における弱毒株の有用性 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=荒尾雄二郎 kn-aut-sei=荒尾 kn-aut-mei=雄二郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END