著者 Miki, Yukari| Gion, Yuka| Mukae, Yuriko| Hayashi, Atsushi| Sato, Hiaki| Yoshino, Tadashi| Takahashi, Kiyoshi|
発行日 2013-02
出版物タイトル European Journal of Haematology
90巻
2号
資料タイプ 学術雑誌論文
著者 Hayashi, Atsushi| Fumon, Takumi| Miki, Yukari| Sato, Hiaki| Yoshino, Tadashi| Takahashi, Kiyoshi|
発行日 2013-06
出版物タイトル Journal of Clinical and Experimental Hematopathology
53巻
1号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/32636
フルテキストURL fulltext.pdf
著者 Kondo, Eisaku| Yoshino, Tadashi| Akagi, Tadaatsu| Hayashi, Kazuhiko| Takahashi, Kiyoshi|
抄録 <p>Southern blot hybridization was used to detect the rearrangement and amplification of five proto-oncogenes (bcl-2, bcl-1, c-myc, c-myb and c-Ha-ras) and one tumor suppressor gene (RB-1) in 55 Japanese patients with non-Hodgkin's lymphoma; 16 with T-cell lymphomas and 39 with B-cell lymphomas (7 follicular and 32 diffuse lymphomas). Genetic abnormalities of the proto-oncogenes were detected in 7 of the 55 (13%). Genetic abnormalities of bcl-2 plus other genes were detected in 5 of 7 cases of follicular lymphoma (71%), rearrangements of bcl-2 and c-myc, rearrangement of bcl-2 and amplification of c-myb. Genetic abnormalities were observed in only three cases of diffuse lymphoma. In each of 3 cases of B-cell lymphoma, one of the genes, blc-2 mbr, bcl-2 mcr and c-myc, was rearranged respectively. The incidence of genetic abnormalities in diffuse lymphomas (6.3%) was lower than that in follicular lymphomas. None of diffuse lymphomas had double oncogene abnormality. No abnormalities were found in RB-1, bcl-1, and Ha-ras. These findings suggest that follicular lymphomas are associated with some abnormalities of oncogenes not restricted to bcl-2 that facilitate growth which may be associated with their clinical features.</p>
キーワード malignant lymphoma cellular oncogenes
Amo Type Article
発行日 1992-12
出版物タイトル Acta Medica Okayama
46巻
6号
出版者 Okayama University Medical School
開始ページ 407
終了ページ 415
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1485535
Web of Sience KeyUT A1992KE49600002
JaLCDOI 10.18926/AMO/32622
フルテキストURL fulltext.pdf
著者 Tanizaki, Yoshiro| Kitani, Hikaru| Okazaki, Morihiro| Mifune, Takashi| Mitsunobu, Fumihiro| Tanimizu, Masakuni| Honke, Naoko| Kusaura, Yasuharu| Ochi, Koji| Harada, Hideo| Soda, Ryo| Takahashi, Kiyoshi| Kimura, Ikuro|
抄録 Twenty-one patients with atopic asthma were classified into three types according to their symptoms (clinical diagnosis): Ia, simple bronchoconstriction; Ib, bronchoconstriction + hypersecretion; and II, bronchiolar obstruction, and this classification was compared with a classification made according to clinical findings and examinations (score diagnosis). Type Ib asthma was characterized by the increased incidence of eosinophils in bronchoalveolar lavage fluid (BALF), while type II was characterized by ventilatory dysfunction in small airways and the increased incidence of neutrophils in BALF. Four patients, whose expectoration was between 50 and 99ml/day, of the 12 with type Ia assessed by clinical diagnosis were evaluated as type Ib by score diagnosis. One patient with type II by clinical diagnosis was assessed as questionable type II by score diagnosis. In the other 16 patients, the clinical and score diagnoses were the same.
キーワード bronchial asthma classification ventilatory function cellular composition of BALF sore diagnosis
Amo Type Article
発行日 1992-08
出版物タイトル Acta Medica Okayama
46巻
4号
出版者 Okayama University Medical School
開始ページ 295
終了ページ 301
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1442153
Web of Sience KeyUT A1992JL44200010
JaLCDOI 10.18926/AMO/32167
フルテキストURL fulltext.pdf
著者 Okazaki, Morihiro| Kitani, Hikaru| Mifune, Takashi| Mitsunobu, Fumihiro| Asaumi, Noboru| Tanizaki, Yoshiro| Harada, Hideo| Takahashi, Kiyoshi| Kimura, Ikuro|
抄録 <p>Serum levels of total IgE, specific IgE, IgG and IgG4 against house dust mite were measured in mite-sensitive asthma patients receiving immunotherapy with house dust. Serum levels of total IgE, mite specific IgE and IgG did not significantly change during the course of hyposensitization. Increased levels of mite specific IgG4 were observed in patients during immunotherapy. The increase in specific IgG4 was dependent on the total dose of house dust administered in both children (r = 0.636, p less than 0.001) and adults (r = 0.629, p less than 0.01). However, the increase of specific IgG4 in adults was not as apparent as in children. These results might suggest that mite specific IgG4 is a useful immunological marker in the immunotherapy for allergic asthma, and that IgG4 antibody acts as a blocking antibody in atopic bronchial asthma.</p>
キーワード house dust mite bronchial asthma hyposensitization specific IgG4
Amo Type Article
発行日 1991-08
出版物タイトル Acta Medica Okayama
45巻
4号
出版者 Okayama University Medical School
開始ページ 267
終了ページ 273
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1962533
Web of Sience KeyUT A1991GD78000009
JaLCDOI 10.18926/AMO/31765
フルテキストURL fulltext.pdf
著者 Ohtsuki, Yuji| Danbara, Yoshifumi| Takeda, Isao| Takahashi, Kiyoshi| Hayashi, Kazuhiko| Sonobe, Hiroshi| Yoshino, Tadashi| Akagi, Tadaatsu|
抄録 <p>Metaplastic bony tissue along with hyperplastic mucosal epithelium showing no atypism was detected in biopsy materials from a Yamada type I gastric polyp. The tissue was metaplastic woven bone associated with calcification. Histogenesis of the bone formation is as yet unknown. This is the first reported case of the presence of metaplastic bone accompanied by hyperplastic gastric mucosa so far.</p>
キーワード stomach hyperplastic polyp metaplastic bone histopathology
Amo Type Article
発行日 1987-02
出版物タイトル Acta Medica Okayama
41巻
1号
出版者 Okayama University Medical School
開始ページ 43
終了ページ 46
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 3105253
Web of Sience KeyUT A1987G146400007
JaLCDOI 10.18926/AMO/31744
フルテキストURL fulltext.pdf
著者 Hayashi, Hisatomo| Tsuda, Takashi| Tsurumi, Naokazu| Takai, Yutaka| Maeda, Masanori| Takahashi, Kiyoshi| Kimura, Ikuro|
抄録 <p>A significant amount of anticoagulant substance was released along with histamine, when human lung mast cells were stimulated with anti-IgE and Ca-ionophore A23187. Its activity was lost by heparinase, not by chondroitin-ABC lyase or chondroitin-AC lyase, and also inhibited by Polybrene, suggesting it would be heparin.</p>
キーワード human lung mast cells heparin anticoagulant activity anti-IgE Ca-ionophore
Amo Type Article
発行日 1987-04
出版物タイトル Acta Medica Okayama
41巻
2号
出版者 Okayama University Medical School
開始ページ 85
終了ページ 87
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2438904
Web of Sience KeyUT A1987H040200006
JaLCDOI 10.18926/AMO/31572
フルテキストURL fulltext.pdf
著者 Tanabe, Kozo| Takahashi, Kiyoshi| Maeda, Masanori| Kimura, Ikuro|
抄録 <p>To confirm the formation of Charcot-Leyden crystals (CLC) in basophils, we observed basophils in sputum and peripheral blood. Sealed slide and suspension culture methods were used to observe the process of CLC formation in peripheral blood basophils and eosinophils under electron microscopy. CLC formation was observed in basophils and eosinophils, and was found to be augmented by sealed slide method. A temperature of 4 degrees C was better than 37 degrees C for promoting the formation of crystals. There was no correlation between the degranulation of these cells and the formation of CLC after stimulation with anti-IgE or anti-IgG antibodies. CLC were initially detected in the cytoplasmic granules of basophils where they continued to enlarge. No CLC were identified in mast cells under any conditions studied. These findings confirm that CLC in sputum are not exclusive to eosinophils and that CLC appear to be present in basophil-rich sites under the cell damage.</p>
キーワード Charcot-Leyden crystals basphils electron microscopy
Amo Type Article
発行日 1993-04
出版物タイトル Acta Medica Okayama
47巻
2号
出版者 Okayama University Medical School
開始ページ 85
終了ページ 90
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8506754
Web of Sience KeyUT A1993LA45200003
JaLCDOI 10.18926/AMO/31532
フルテキストURL fulltext.pdf
著者 Tanizaki, Yoshiro| Komagoe, Haruki| Sudo, Michiyasu| Morinaga, Hiroshi| Kitani, Hikaru| Nakagawa, Saburo| Matsuoka, Takashi| Tada, Shinya| Takahashi, Kiyoshi| Kimura, Ikuro|
抄録 <p>Candida albicans-induced histamine release from basophils was studied in 54 patients with bronchial asthma in comparison with the release caused by house dust and anti-IgE. The release of histamine induced by C. albicans and that induced by house dust were closely related to the serum levels of specific IgE antibodies as expressed by RAST scores. A correlation of C. albicans-induced histamine release with the release caused by anti-IgE was not generally observed. On the other hand, a close correlation was found between house dust- and anti-IgE-induced histamine release. It was suggested from these results that the differences between C. albicans- and house dust-induced histamine release might be due to the different antigenicity of the two allergens.</p>
キーワード histamine release C. albicans house dust anti-IgE antigeniciy
Amo Type Article
発行日 1985-06
出版物タイトル Acta Medica Okayama
39巻
3号
出版者 Okayama University Medical School
開始ページ 191
終了ページ 197
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2411109
Web of Sience KeyUT A1985ALG3300004
JaLCDOI 10.18926/AMO/31498
フルテキストURL fulltext.pdf
著者 Tanizaki, Yoshiro| Komagoe, Haruki| Morinaga, Hiroshi| Ohtani, Jun| Nakagawa, Saburo| Maeda, Masanori| Kitani, Hikaru| Takahashi, Kiyoshi| Kimura, Ikuro|
抄録 <p>The inhibitory effect of nicardipine, a calcium antagonist, on the antigen- and anti-IgE-induced histamine release from basophilic leucocytes of patients with bronchial asthma was examined. The agent significantly inhibited both antigen-stimulated and anti-IgE-induced histamine release from basophils (the maximum percent inhibition was 57.8 +/- 7.2% and 56.0 +/- 8.8%, respectively). Pre-incubation of basophils with nicardipine for periods of up to 120 min did not alter the inhibitory effect. These results suggest that nicardipine modifies the histamine release from basophils which closely participate in an attack of bronchial asthma.</p>
キーワード histamine release basophils antigen anti-IgE Ca<sup>2+</sup> antagonist
Amo Type Article
発行日 1985-08
出版物タイトル Acta Medica Okayama
39巻
4号
出版者 Okayama University Medical School
開始ページ 247
終了ページ 251
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2413722
Web of Sience KeyUT A1985APN0700001
JaLCDOI 10.18926/AMO/31108
フルテキストURL fulltext.pdf
著者 Miyatani, Katsuya| Takahashi, Kiyoshi| Yanai, Hiroyuki| Yoshino, Tadashi| Akagi, Tadaatsu|
抄録 <p>Previously, we reported that interleukin-2 (IL-2)-stimulated helper T cells produced an unknown soluble factor which induced dendritic cell-like differentiation in primary cultures of monocytic leukemia cells and we referred to this factor as dendritic cell differentiation factor (DCDF). In this study, we attempted to purify and characterize DCDF and investigated its biological effect on normal human monocytes. Gel filtration chromatography indicated that the molecular weight of DCDF is approximately 30-35 kDa. Chromatofocusing indicated that the isoelectric point of DCDF is approximately 5.0. DCDF, partially purified by subsequent gel filtration, chromatofocusing, and hydrophobic chromatography, significantly enhanced the HLA-DR expression of normal human monocytes and a human monocytic leukemia cell line, THP-1. This biological activity was not neutralized by any known antibodies to human cytokines. DCDF significantly amplified the T-cell stimulatory activity of monocytes in the allogeneic mixed leukocyte reaction (MLR). Moreover, DCDF significantly enhanced IL-1 beta and IL-6 production by monocytes in a dose-dependent manner. These results suggest that DCDF is a novel human cytokine which stimulates the accessory cell function of monocytes.</p>
キーワード dendritic cell differentiation protein purification cytokine
Amo Type Article
発行日 1994-04
出版物タイトル Acta Medica Okayama
48巻
2号
出版者 Okayama University Medical School
開始ページ 67
終了ページ 72
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8042536
Web of Sience KeyUT A1994NJ77500001
JaLCDOI 10.18926/AMO/31091
フルテキストURL fulltext.pdf
著者 Teramoto, Norihiro| Tonoyama, Yuji| Akagi, Tadaatsu| Sarker, Ashit Baran| Yoshino, Tadashi| Yamadori, Ichiro| Takahashi, Kiyoshi|
抄録 <p>The sensitivity and specificity of single cell polymerase chain reaction (PCR) were studied. Its high sensitivity enabled detection of a single-copy gene, such as human T-lymphotropic virus type I genome in paraffin sections. The rate of obtaining positive signals with this method was affected by the number of copies of the gene in the target cell. Specificity was satisfactory if the procedure was properly and carefully followed. Since the single cell PCR is a time-consuming method which requires skill and experience to pick up the target cells accurately, the applicability of this method is limited. It works best when it is used to analyze a single or a few copy genes in histologically identified cells.</p>
キーワード polymerase chain reaction human T-lymphotropic virus type I paraffin section single cell single copy gene
Amo Type Article
発行日 1994-08
出版物タイトル Acta Medica Okayama
48巻
4号
出版者 Okayama University Medical School
開始ページ 189
終了ページ 193
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 7817773
Web of Sience KeyUT A1994PE51400003
著者 Ozaki, Hiroshi| Mizutani, Machiko| Hayashi, Hiromitsu| Oka, Eiji| Ohtahara, Shunsuke| Kimoto, Hiroshi| Tanaka, Toshio| Hakozaki, Hand| Takahashi, Kiyoshi| Suzuki, Yoshiyuki|
発行日 1978-04
出版物タイトル Acta Medica Okayama
32巻
1号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/30993
フルテキストURL fulltext.pdf
著者 Tanizaki, Yoshiro| Sudo, Michiyasu| Kitani, Hikaru| Araki, Hiroyuki| Oki, Kazuhiko| Tsuji, Mitsuaki| Takahashi, Kiyoshi| Kimura, Ikuro|
抄録 <p>The arylsulfatase activity and histamine concentration of bronchoalveolar lavage fluid (BALF) were examined in patients with bronchial asthma in relation to the eosinophil count and asthma type (atopic and non-atopic). The BALF arylsulfatase activity and histamine concentration were significantly higher in atopic asthmatics than in non-atopic asthmatics. In atopic asthmatics, the activity of arylsulfatase was significantly increased in patients with a higher eosinophil count (10% or more). However, the BALF histamine concentration did not correlate with the eosinophil count. In non-atopic asthmatics, there was no significant correlation between arylsulfatase activity and the eosinophil count. The results show that arylsulfatase participates in IgE-mediated allergic reactions.</p>
キーワード eosinophil count arylsulfatase histamine bronchoalveolar lavage fluid bronchial asthma
Amo Type Article
発行日 1988-08
出版物タイトル Acta Medica Okayama
42巻
4号
出版者 Okayama University Medical School
開始ページ 227
終了ページ 230
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 3177008
Web of Sience KeyUT A1988P884600006
著者 Kimura, Ikuro| Tanizaki, Yoshiro| Sato, Shuichi| Saito, Katsuyoshi| Takahashi, Kiyoshi| Ueda, Nobuo| Ono, Hatsuko| Moritani, Yoshiaki|
発行日 1975-02
出版物タイトル Acta Medica Okayama
29巻
1号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/30874
フルテキストURL fulltext.pdf
著者 Sonobe, Hiroshi| Hayashi, Kazuhiko| Takahashi, Kiyoshi|
抄録 <p>A case of ovarian leiomyoma is reported, together with histologic, immunohistologic and electron microscopic findings. A solid firm tumor, measuring 6.5 X 5 X 5 cm, was found in the right ovary of a 65-year-old woman. The tumor had an obvious whorled pattern on the cut-surface. Well-differentiated, long spindle-shaped neoplastic cells revealed positive immunoreactivity for anti-desmin. Ultrastructural observations included numerous microfilaments with dense patches in the cytoplasm, micropinocytotic vesicles beneath plasma membranes and continuous basal laminae around neoplastic cells. These findings were compatible with leiomyoma. The possible histogenesis of ovarian leiomyoma was discussed.</p>
キーワード leiomyoma ovary immunohistochemistry ultrastructure
Amo Type Article
発行日 1989-04
出版物タイトル Acta Medica Okayama
43巻
2号
出版者 Okayama University Medical School
開始ページ 131
終了ページ 134
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2728906
Web of Sience KeyUT A1989U578500008
JaLCDOI 10.18926/AMO/30854
フルテキストURL fulltext.pdf
著者 Akagi, Tadaatsu| Nose, Soichiro| Takahashi, Kiyoshi| Yoshino, Tadashi| Horie, Yasushi| Motoi, Makoto| Sonobe, Hiroshi| Enzan, Hideaki|
抄録 <p>In the human lymphoreticular system, the alpha and beta subunits of S-100 protein are found in ordinary monocyte-macrophages and non-phagocytic histiocytes such as Langerhans cells and interdigitating reticulum cells, respectively. The beta subunit is also present in some CD8+ T cells. In the present study, we investigated the ontogeny of these histiocytes and lymphocytes in humans. Yolk sacs and 4 to 21-week fetuses were examined immunohistochemically for the presence of S-100 protein subunits using antisera monospecific to each subunit. S-100 alpha + macrophages were present in the yolk sacs and the hepatic sinusoids of the 4th week embryos prior to bone marrow hematopoiesis. These macrophages later appeared in other lymphoid organs when anlagen of these organs were formed. No S-100 beta + cells were found in the yolk sacs. S-100 beta+ histiocytes were first detected in the hepatic sinusoids of the 5th week embryo, and after the 8th week of gestation, they were distributed in other lymphoid organs. S-100 beta+ lymphocytes were not found in the liver. They were first detected in the thymus at the 12th week of gestation, and were subsequently distributed in other lymphoid organs. These results suggest that S-100 beta+ lymphocytes and histiocytes may belong to different cell lineages, and the former may not be the precursor of the latter.</p>
キーワード S-100 protein ontogeny lymphocyte histiocyte
Amo Type Article
発行日 1989-08
出版物タイトル Acta Medica Okayama
43巻
4号
出版者 Okayama University Medical School
開始ページ 203
終了ページ 210
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2678903
Web of Sience KeyUT A1989AP79100002
JaLCDOI 10.18926/AMO/30764
フルテキストURL fulltext.pdf
著者 Ino, Hideo| Hayashi, Kazuhiko| Yanai, Hiroyuki| Teramoto, Norihiro| Koirala, Tirtha Raj| Chen, Hong-Li| Oka, Takashi| Yoshino, Tadashi| Takahashi, Kiyoshi| Akagi, Tadaastu|
抄録 <p>A simian cell line, Si-IIA, harboring Epstein-Barr-virus (EBV) -related herpesvirus (Si-IIA-EBV), produces malignant lymphoma in rabbits when administered by intravenous inoculation. In this study, we analyzed the Si-IIA-EBV genome and compared it with human EBV and herpesvirus macaca fascicularis 1 (HVMF 1 ), which is associated with B-cell lymphoma developing in SIV-infected immunosuppressed monkeys. DNA from Si-IIA-EBV was amplified by the polymerase chain reaction using three different primer pairs complementary to human EBV (B95-8) DNA; two of the primer pairs covered part of the long internal repeat 1 region (IR 1) and the third covered part of the BRRF 1 region. Direct sequencing of the three PCR products revealed that Si-IIA-EBV DNA had about 82% nucleotide homology to the human EBV DNA in all three regions and 92.4% homology to HVMF1 in the IR1 region. The blotting pattern by Southern blot analysis was different between Si-IIA-EBV and human EBV.</p>
キーワード Epstein-Barr virus HVMF 1 lymphoma ?monkey cell line PCR
Amo Type Article
発行日 1997-08
出版物タイトル Acta Medica Okayama
51巻
4号
出版者 Okayama University Medical School
開始ページ 207
終了ページ 212
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9284968
Web of Sience KeyUT A1997XU03200004
JaLCDOI 10.18926/AMO/30530
フルテキストURL fulltext.pdf
著者 Tanizaki, Yoshiro| Takahashi, Kiyoshi| Goda, Yoshinori| Sasaki, Yoshihede| Harada, Hiroshi| Kimura, Ikuro|
抄録 <p>Sixty-four patients with confirmed bronchial asthma were treated with HC 20-511 (Ketotifen). HC20-511 was evaluated to be very effective in 6.3%, effective in 50.0% and slightly effective in 10.9% of these patients. The appearance of reactive basophils was inhibited by HC 20-511 in 5 out of 6 cases of reaction to house dust, in all three cases with buckwheat allergy to their allergen and in 7 out of 11 cases to anti-IgE. These results confirm that HC 20-511 inhibits type I allergic reactions induced by specific allergen and IgE.</p>
キーワード bronchial asthma ketotifen basophil reactivity allergens anti-IgE
Amo Type Article
発行日 1980-12
出版物タイトル Acta Medica Okayama
34巻
6号
出版者 Okayama University Medical School
開始ページ 383
終了ページ 388
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6451144
Web of Sience KeyUT A1980KZ17800004
JaLCDOI 10.18926/AMO/30509
フルテキストURL fulltext.pdf
著者 Yanai, Hiroyuki| Yoshino, Tadashi| Takahashi, Kiyoshi| Ninomiya, Yoshifumi| Akagi, Tadaatsu|
抄録 <p>Circulating hepatitis C virus (HCV) particles can be fractionated by means of differential flotation centrifugation. It is reported that in the bottom fraction HCV is in the form immune complexes, whereas in the top, it is free of antibodies. We evaluated the significance of circulating complex and free HCV in chronic hepatitis C, and assessed the relationship in terms of the response to interferon (IFN) therapy. We examined sera before, just after, and 1 year after administering IFN to 18 patients with chronic hepatitis C, 10 of whom responded (group CR), and 8 did not (group NR). The amounts of virus were similar between both groups before therapy. After differential flotation centrifugation with 1.063 g/ml of NaCl, the top and bottom fractions were assayed for HCV RNA. Before therapy, HCV RNA was detected in the top fraction in 1 of 10 in group CR, and in 6 of 8 in group NR (P &#60; 0.05, chi-square test). HCV RNA was positive in the bottom fraction of all samples. In a follow-up study of group NR, HCV RNA was detected in the top fraction in 3 of 8 just after IFN therapy, and in 7 of 8 after 1 year. This study suggests that the presence of HCV in the top fraction can predict a poor response to IFN therapy.</p>
キーワード IL-2R ??chain phorbol ester monocyte differentiation protein kinase
Amo Type Article
発行日 1996-06
出版物タイトル Acta Medica Okayama
50巻
3号
出版者 Okayama University Medical School
開始ページ 145
終了ページ 150
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8805853
Web of Sience KeyUT A1996UU60400005