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ID 32990
フルテキストURL
Thumnail fig.pdf 147 KB
著者
Yoshihara, Ritsuko Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Aoyama, Eriko Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kadota, Yusuke Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kawai, Saeko Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Goto, Tomomi Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Zhong, Ming Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Gohda, Eiichi Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
抄録

A two-step culture system was used to investigate the role of chondroitin sulfate (CS) B, which is mitogenic to B cells, in differentiation of B cells. Mouse spleen B cells were incubated for 3 days with CSB in the presence of interleukin (IL)-4 and IL-5. After washing, the cells were replated at 10(5) viable cells/well and recultured without CSB in the presence of IL-4 and IL-5. CSB dose-dependently increased IgM production, the greatest enhancement being 450%. Dextran sulfate had a similar effect, whereas other glycosaminoglycans, CSA, CSC, heparin and hyaluronic acid, were marginally effective. Treatment of B cells with CSB resulted in increases in the number of IgM-secreting cells and numbers of CD138-positive cells and CD45R/B220-negative cells. CSB-induced IgM production was inhibited by the protein kinase C (PKC) inhibitor GF109203X but not by the phosphatidylinositol 3-kinase (P13K) inhibitor wortmannin. These results demonstrated that CSB promoted differentiation of B cells in the presence of IL-4 and IL-5 and suggested that PKC but not P13K is crucial for CSB-induced IgM production.

キーワード
chondroitin sulfate B (CSB)
murine B cells
IgM
differentiation
CD138
protein kinase C
備考
Published with permission from the copyright holder.
This is a author's copy,as Cellular Immunology, 2007 Vol.250 Issue.1-2 pp.14-23
Publisher URL: http://dx.doi.org/10.1016/j.cellimm.2007.12.002
Direct access to Thomson Web of Science record
Copyright © 2007 by Elsevier Inc.
発行日
2008-06-30
出版物タイトル
Cellular Immunology
250巻
1-2号
開始ページ
14
終了ページ
23
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
査読
有り
DOI
Web of Sience KeyUT
Submission Path
biology_general/40