フルテキストURL Mod_Rheumatol_26_6_940.pdf fig.pdf tbl.jpg fig_all.zip
著者 Hara, Chikako| Kubota, Satoshi| Nishida, Takashi| Hiasa, Miki| Hattori, Takako| Aoyama, Eriko| Moriyama, Yoshinori| Kamioka, Hiroshi| Takigawa, Masaharu|
抄録 OBJECTIVES: Platelet-rich plasma (PRP) has been widely used to enhance the regeneration of damaged joint tissues, such as osteoarthritic and rheumatoid arthritic cartilage. The aim of this study is to clarify the involvement of all of the CCN family proteins that are crucially associated with joint tissue regeneration. METHODS: Cyr61-CTGF-NOV (CCN) family proteins in human platelets and megakaryocytic cells were comprehensively analyzed by Western blotting analysis. Production of CCN family proteins in megakaryocytes in vivo was confirmed by immunofluorescence analysis of mouse bone marrow cells. Effects of CCN family proteins found in platelets on chondrocytes were evaluated by using human chondrocytic HCS-2/8 cells. RESULTS: Inclusion of CCN2, a mesenchymal tissue regenerator, was confirmed. Of note, CCN3, which counteracts CCN2, was newly found to be encapsulated in platelets. Interestingly, these two family members were not detectable in megakaryocytic cells, but their external origins were suggested. Furthermore, we found for the first time CCN5 and CCN1 that inhibits ADAMTS4 in both platelets and megakaryocytes. Finally, application of a CCN family cocktail mimicking platelets onto HCS-2/8 cells enhanced their chondrocytic phenotype. CONCLUSIONS: Multiple inclusion of CCN1, 2 and 3 in platelets was clarified, which supports the harmonized regenerative potential of PRP in joint therapeutics.
キーワード CCN family Cartilage Megakaryocyte Platelet Regeneration
備考 This is an Accepted Manuscript of an article published by Taylor & Francis Group This fulltext availavle in Nov 2017
発行日 2016-11
出版物タイトル Modern Rheumatology
26巻
6号
出版者 Taylor & Francis
開始ページ 940
終了ページ 949
ISSN 1439-7595
NCID AA1157187X
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 26915735
DOI 10.3109/14397595.2016.1155255
Web of Sience KeyUT 000393121500022
関連URL isVersionOf https://doi.org/10.3109/14397595.2016.1155255
著者 青山 絵理子|
発行日 2015-02
出版物タイトル Okayama University Medical Research Updates
6巻
資料タイプ その他
著者 Tomita, Nao| Hattori, Takako| Itoh, Shinsuke| Aoyama, Eriko| Yao, Mayumi| Yamashiro, Takashi| Takigawa, Masaharu|
発行日 2013-03-28
出版物タイトル PLoS ONE
8巻
3号
資料タイプ 学術雑誌論文
著者 Itoh, Shinsuke| Hattori, Takako| Tomita, Nao| Aoyama, Eriko| Yutani, Yasutaka| Yamashiro, Takashi| Takigawa, Masaharu|
発行日 2013-08-12
出版物タイトル PLoS ONE
8巻
8号
資料タイプ 学術雑誌論文
著者 Hoshijima, Mitsuhiro| Hattori, Takako| Aoyama, Eriko| Nishida, Takashi| Yamashiro, Takashi| Takigawa, Masaharu|
発行日 2012-10
出版物タイトル FEBS Journal
279巻
19号
資料タイプ 学術雑誌論文
著者 Yoshihara, Ritsuko| Aoyama, Eriko| Kadota, Yusuke| Kawai, Saeko| Goto, Tomomi| Zhong, Ming| Gohda, Eiichi|
発行日 2008-06-30
出版物タイトル Cellular Immunology
250巻
1-2号
資料タイプ 学術雑誌論文
著者 青山 絵理子|
発行日 2005-03-25
出版物タイトル
資料タイプ 学位論文