ID | 31719 |
JaLCDOI | |
フルテキストURL | |
著者 |
Kondo, Asami
Okayama University
Makino, Eiichi
Okayama University
Namba, Masayoshi
Okayama University
Okada, Shigeru
Okayama University
Huh, Nam-ho
Okayama University
|
抄録 | Using 2-dimensional gel electrophoresis, we previously demonstrated that the S100C protein remarkably decreased after immortalization of normal human fibroblasts, and that this protein caused growth inhibition of human tumor cells when forcibly expressed in these cells, suggesting that S100C plays a significant role in tumor suppression. The present study was carried out to determine what type of human tissues express S100C protein, and, subsequently, whether the S100C content in these tissues changes after normal cells have been transformed into cancer cells. We found that ductal cells in various tissues were positively stained with the S100C protein. In comparison, epithelial cells in digestive organs such as the stomach, small intestine, and colon were not stained as strongly. When 14 pairs of human normal and cancerous tissues were stained with the antibody, decreases in the staining levels of S100C were observed in 6 kinds of cancerous tissues--from the bronchus, mammary duct, renal tubule, prostate, uterus, and testis--in comparison with staining in their normal counterparts. These results suggest that S100C is a new tumor marker protein, the expression of which significantly decreases after malignant transformation of human tissues. |
キーワード | S100C-antibody
human tissues
immunostaining
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 2002-02
|
巻 | 56巻
|
号 | 1号
|
出版者 | Okayama University Medical School
|
開始ページ | 31
|
終了ページ | 34
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
PubMed ID | |
Web of Science KeyUT |