ID | 10604 |
Eprint ID | 10604
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フルテキストURL | |
タイトル(別表記) | Glutamate Snsor Using L-Glutamate Oxidase and Its Application for Sensing GOT/GPT Activity
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著者 |
有馬 二朗
岡山大学
篠原 寛明
岡山大学
日下部 均
ヤマサ醤油株式会社
田中 英彦
岡山大学
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抄録 | L-Glutamate measurement and GOT/GPT assay was successful by H2O2 measurement using the L-glutamate oxidase with 4-aminoantipyrine / phenol method. But, in examination of oxigen electrode, immobilized L-glutamate oxidase at the cellulose to L-glutamate and GOT-GPT sensor, Lpglutamate measurement was used for the amperometric determination with non-fixed enzyme. On examination of electron mediator, response for L-glutamate was observed with each of the compounds ferricyane, ferrocene-COOH, ferrocene-MeOH, and benzoquinone. L-Glutamate was measured by carbon printed tip electrode the L-glutamate oxidase and ferricyane based on the principle of chronoamperometry. A linear calibration graph was obtained between 1mM and 30mM. These results suggest that L-glutamate oxidase is able to utilize to L-glutamate sensor, and that there is a strong possibility to put this sensor to sensing for GOT/GPT activity.
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キーワード | L-glutamate ocidase
L-glutamate sensor
GOT/GPT sensor
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発行日 | 2002-02
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出版物タイトル |
岡山大学農学部学術報告
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出版物タイトル(別表記) | Scientific reports of the Faculty of Agriculture, Okayama University
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巻 | 91巻
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号 | 1号
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出版者 | 岡山大学農学部
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出版者(別表記) | Faculty of Agriculture,Okayama University
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開始ページ | 15
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終了ページ | 22
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ISSN | 0474-0254
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NCID | AN00033029
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資料タイプ |
紀要論文
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言語 |
日本語
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論文のバージョン | publisher
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査読 |
無し
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Eprints Journal Name | srfa
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