JaLCDOI 10.18926/AMO/52405
フルテキストURL 68_2_89.pdf
著者 Sako, Shinichi| Kariyama, Reiko| Mitsuhata, Ritsuko| Yamamoto, Masumi| Wada, Koichiro| Ishii, Ayano| Uehara, Shinya| Kokeguchi, Susumu| Kusano, Nobuchika| Kumon, Hiromi|
抄録 We conducted a study on molecular epidemiology and clinical implications of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa isolated from urine. Over a 10-year period from 2001 through 2010, a total of 92 MBL-producing P. aeruginosa urine isolates were collected from patients (one isolate per patient) who were admitted to 5 hospitals in Okayama Prefecture, Japan. When cross-infection was suspected in the hospital, pulsed-field gel electrophoresis was performed. In the resulting dendrogram of 79 MBL-producing P. aeruginosa urine isolates, no identical isolates and 7 pairs of isolates with ≥80% similarity were found. The biofilm-forming capabilities of 92 MBL-producing P. aeruginosa urine isolates were significantly greater than those of 92 non-MBL-producing urine isolates in a medium of modified artificial urine. The imipenem resistance transferred in 16 of 18 isolates tested, and these frequencies were in the range of 10-3 to 10-9. All of 18 isolates tested belonged to internationally spread sequence type 235 and had 3 gene cassettes of antimicrobial resistance genes in the class 1 integron. The strong biofilm-forming capabilities of MBL-producing P. aeruginosa urine isolates could be seriously implicated in nosocomial infections. To prevent spread of the organism and transferable genes, effective strategies to inhibit biofilm formation in medical settings are needed.
キーワード Pseudomonas aeruginosa metallo-β-lactamase molecular epidemiology biofilm urinary tract infection
Amo Type Original Article
発行日 2014-04
出版物タイトル Acta Medica Okayama
68巻
2号
出版者 Okayama University Medical School
開始ページ 89
終了ページ 99
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 CopyrightⒸ 2014 by Okayama University Medical School
論文のバージョン publisher
査読 有り
Submission Path amo/vol68/iss2/4
PubMed ID 24743784
Web of Sience KeyUT 000334652700004
関連URL http://ousar.lib.okayama-u.ac.jp/metadata/52504
JaLCDOI 10.18926/AMO/32863
フルテキストURL fulltext.pdf
著者 Murao, Wataru| Wada, Koichiro| Matsumoto, Akira| Fujiwara, Michihisa| Fukushi, Hideto| Kishimoto, Toshio| Monden, Koichi| Kariyama, Reiko| Kumon, Hiromi|
抄録 <p>In 2000, chlamydial strains OK133 and OK135 were isolated from 2 female patients with cervicitis. These strains were unresponsive to commercially available PCR and LCR test kits for the diagnosis of Chlamydia trachomatis infection, and their phenotypic characteristics were very similar. The OK135 nucleotide sequence in MOMP-VD2 gene closely resembled that of Chlamydophila caviae GPIC. A similar strain was isolated in 2003 from a male patient OKM2 with urethritis, from which the strain SC10-6 was cloned by the plaque purification method. The nucleotide sequence of the entire MOMP gene of SC10-6 was exactly the same as that of OK135. Thus, the strains OK135 and SC10-6, together with OK133, have been called C. caviae-like Chlamydia. We designed primers for nested PCR assay, the product of which showed a single-band 311-bp fragment, to detect C. caviae-like Chlamydia. Of swab specimens obtained from 202 patients from 2003 to 2006 (119 male and 83 female patients), 18 specimens (8.9%) from 14 male and 4 female patients were positive, suggesting that C. caviae-like Chlamydia infection is rather common. Thus far, it has not been determined whether C. caviae-like Chlamydia is pathogenic for humans.</p>
キーワード Chlamydophila caviae-like Chlamydia urethra uterine cervix epidemiology sexually transmitted infection
Amo Type Original Article
発行日 2010-02
出版物タイトル Acta Medica Okayama
64巻
1号
出版者 Okayama University Medical School
開始ページ 1
終了ページ 9
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
論文のバージョン publisher
査読 有り
Submission Path amo/vol64/iss1/1
PubMed ID 20200578
Web of Sience KeyUT 000274868300001
著者 Ando, Eiichi| Monden, Koichi| Mitsuhata, Ritsuko| Kariyama, Reiko| Kumon, Hiromi|
発行日 2004-08
出版物タイトル Acta Medica Okayama
58巻
4号
資料タイプ 学術雑誌論文
著者 Seno, Yuko| Kariyama, Reiko| Mitsuhata, Ritsuko| Monden, Koichi| Kumon, Hiromi|
発行日 2005-06
出版物タイトル Acta Medica Okayama
59巻
3号
資料タイプ 学術雑誌論文
著者 Mikuniya, Takeshi| Kato, Yoshihisa| Kariyama, Reiko| Monden, Koichi| Hikida, Muneo| Kumon, Hiromi|
発行日 2005-10
出版物タイトル Acta Medica Okayama
59巻
5号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/31836
フルテキストURL fulltext.pdf
著者 Wada, Koichiro| Kariyama, Reiko| Mitsuhata, Ritsuko| Uehara, Shinya| Watanabe, Toyohiko| Monden, Koichi| Kumon, Hiromi|
抄録 <p>Urinary tract infections (UTIs) due to fluoroquinolone-insusceptible Escherichia coli have become increasingly common in recent years. We investigated the potential relationships between clinical measures to combat fluoroquinolone-insusceptible E. coli and experimental analyses on E. coli isolates. Over a 14-year period from 1994 through 2007, a total of 828 E. coli isolates were collected from patients (one isolate per patient) with UTI at the urology ward of Okayama University Hospital. We analyzed the mutations in quinolone resistance-determining regions of DNA gyrase (gyrA) and topoisomerase IV (parC). The production of biofilm by these isolates was also examined and the associated medical records were retrospectively reviewed. Seven of 189 (3.7%) strains from uncomplicated UTIs and 82 of 639 (12.8%) strains from complicated UTIs were insusceptible to fluoroquinolones. Amino acid replacements of type II topoisomerases were frequently observed at positions 83 and 87 in GyrA and at positions 80 and 84 in ParC. No significant difference in the biofilm-forming capabilities was observed between fluoroquinolone-susceptible and -insusceptible E. coli. Our study suggests that biofilm formation of fluoroquinolone-insusceptible E. coli isolates is not a major mechanism of resistance in patients with UTI.</p>
キーワード fluoroquinolone Escherichia coli biofilm MICs QRDRs
Amo Type Original Article
発行日 2009-10
出版物タイトル Acta Medica Okayama
63巻
5号
出版者 Okayama University Medical School
開始ページ 263
終了ページ 272
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
論文のバージョン publisher
査読 有り
Submission Path amo/vol63/iss5/6
PubMed ID 19893602
Web of Sience KeyUT 000271132000006
著者 狩山 玲子|
発行日 1983-04-30
出版物タイトル 岡山医学会雑誌
95巻
3-4号
資料タイプ 学術雑誌論文
著者 藤井 順子| 木口 健一郎| 永田 哲英| 狩山 玲子| 平井 義一| 金政 泰弘| 小林 純|
発行日 1983-02-28
出版物タイトル 岡山医学会雑誌
95巻
1-2号
資料タイプ 学術雑誌論文
著者 犬飼 昌子| 野村 佳代| 渡邉 久美| 千田 好子| 光畑 律子| 狩山 玲子|
発行日 2006-03-31
出版物タイトル 岡山大学医学部保健学科紀要
16巻
2号
資料タイプ 紀要論文
著者 狩山 玲子|
発行日 1983-03-31
出版物タイトル
資料タイプ 学位論文