著者 Asanuma, Masato| Miyazaki, Ikuko| Francisco J., Diaz-Corrales| Higashi, Youichirou| Namba, Masayoshi| Ogawa, Norio|
発行日 2013-06-12
出版物タイトル PLOS ONE
8巻
6号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/53020
フルテキストURL 68_6_317.pdf
著者 Kasahara, Kyosuke| Miyoshi, Ko| Murakami, Shinki| Miyazaki, Ikuko| Asanuma, Masato|
抄録 In vertebrates, almost all somatic cells extend a single immotile cilium, referred to as a primary cilium. Increasing evidence suggests that primary cilia serve as cellular antennae in many types of tissues by sensing chemical or mechanical stimuli in the milieu surrounding the cells. In rodents an antibody to adenylyl cyclase 3 (AC3) has been widely used to label the primary cilia of neurons in vivo by immunostaining, whereas the lack of markers for the primary cilia of astrocytes has made it difficult to observe astrocytic primary cilia in vivo. Here, we obtained a visualization of astrocytic primary cilia in the mouse brain. In the somatosensory cortex, a large portion of neurons and astrocytes at postnatal day 10 (P10), and of neurons at P56 had AC3-positive primary cilia, whereas only approx. one-half of the astrocytes in the P56 mice carried primary cilia weakly positive for AC3. In contrast, the majority of astrocytes had ADP-ribosylation factor-like protein 13B (Arl13b)-positive primary cilia in the somatosensory cortex and other brain regions of P56 mice. The lengths of astrocytic primary cilia positive for Arl13b varied among the brain regions. Our data indicate that Arl13b is a noteworthy marker of astrocytic primary cilia in the brain.
キーワード primary cilia astrocyte ADP-ribosylation factor-like protein 13B
Amo Type Original Article
発行日 2014-12
出版物タイトル Acta Medica Okayama
68巻
6号
出版者 Okayama University Medical School
開始ページ 317
終了ページ 322
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 CopyrightⒸ 2014 by Okayama University Medical School
論文のバージョン publisher
査読 有り
Submission Path amo/vol68/iss6/1
PubMed ID 25519025
Web of Sience KeyUT 000346882200001
著者 Murakami, Shinki| Miyazaki, Ikuko| Sogawa, Norio| Miyoshi, Ko| Asanuma, Masato|
発行日 2014-10
出版物タイトル Neurotoxicity Research
26巻
3号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/47009
フルテキストURL 65_5_279.pdf
著者 Miyoshi, Ko| Kasahara, Kyosuke| Miyazaki, Ikuko| Asanuma, Masato|
抄録 Almost all mammalian cells carry one primary cilium that functions as a biosensor for chemical and mechanical stimuli. Genetic damages that compromise cilia formation or function cause a spectrum of disorders referred to as ciliapathies. Recent studies have demonstrated that some pharmacological agents and extracellular environmental changes can alter primary cilium length. Renal injury is a well-known example of an environmental insult that triggers cilia length modification. Lithium treatment causes primary cilia to extend in several cell types including neuronal cells;this phenomenon is likely independent of glycogen synthase kinase-3β inhibition. In renal epithelial cell lines, deflection of the primary cilia by fluid shear shortens them by reducing the intracellular cyclic AMP level, leading to a subsequent decrease in mechanosensitivity to fluid shear. Primary cilium length is also influenced by the dynamics of actin filaments and microtubules through the levels of soluble tubulin in the cytosol available for primary cilia extension. Thus, mammalian cells can adapt to the extracellular environment by modulating the primary cilium length, and this feedback system utilizing primary cilia might exist throughout the mammalian body. Further investigation is required concerning the precise molecular mechanisms underlying the control of primary cilium length in response to environmental factors.
キーワード primary cilium length lithium cyclic AMP soluble tubulin intraflagellar transport
Amo Type Review
発行日 2011-10
出版物タイトル Acta Medica Okayama
65巻
5号
出版者 Okayama University Medical School
開始ページ 279
終了ページ 285
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 CopyrightⒸ 2011 by Okayama University Medical School
論文のバージョン publisher
査読 有り
Submission Path amo/vol65/iss5/1
PubMed ID 22037264
Web of Sience KeyUT 000296116400001
JaLCDOI 10.18926/AMO/40129
フルテキストURL 64_4_219.pdf
著者 Doi, Maho| Miyazaki, Ikuko| Nagamachi, Tomoko| Shinomiya, Kazuaki| Matsunaga, Hisashi| Sendo, Toshiaki| Kawasaki, Hiromu| Asanuma, Masato| Gomita, Yutaka| Kitamura, Yoshihisa|
抄録 We examined the influence of chronic adrenocorticotropic hormone (ACTH) treatment on the number of Ki-67-positive cells in the dentate gyrus of the hippocampus in rats. ACTH treatment for 14 days decreased the number of such cells. The administration of imipramine or lithium alone for 14 days had no effect in saline-treated rats. The effect of ACTH was blocked by the administration of imipramine. Furthermore, the coadministration of imipramine and lithium for 14 days significantly increased the number of Ki-67-positive cells in both the saline and ACTH-treated rats. The coadministration of imipramine and lithium normalized the cell proliferation in the dentate gyrus of the hippocampus in rats treated with ACTH.
キーワード ACTH imipramine lithium proliferation Ki-67
Amo Type Original Article
発行日 2010-08
出版物タイトル Acta Medica Okayama
64巻
4号
出版者 Okayama University Medical School
開始ページ 219
終了ページ 223
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 Okayama University Medical School
論文のバージョン publisher
査読 有り
Submission Path amo/vol64/iss4/2
PubMed ID 20802538
Web of Sience KeyUT 000281384400002
著者 Tanaka, Ken-ichi| Fujita, Naoko| Asanuma, Masato| Ogawa, Norio|
発行日 2000-12
出版物タイトル Acta Medica Okayama
54巻
6号
資料タイプ 学術雑誌論文
著者 Takayama, Haruhiko| Ogawa, Norio| Asanuma, Masato| Hirata, Hiroshi| Ogura, Toshio| Ota, Zensuke|
発行日 1991-10
出版物タイトル Acta Medica Okayama
45巻
5号
資料タイプ 学術雑誌論文
著者 Asanuma, Masato| Miyazaki, Ikuko| Diaz-Corrales, Francisco J| Ogawa, Norio|
発行日 2004-10
出版物タイトル Acta Medica Okayama
58巻
5号
資料タイプ 学術雑誌論文
著者 Aoki, Sogawa Chiharu| Asanuma, Masato| Sogawa, Norio| Miyazaki, Ikuko| Nakanishi, Tohru| Furuta, Hiroaki| Ogawa, Noriko|
発行日 2001-02
出版物タイトル Acta Medica Okayama
55巻
1号
資料タイプ 学術雑誌論文
著者 Miyazaki, Ikuko| Asanuma, Masato|
発行日 2008-06
出版物タイトル Acta Medica Okayama
62巻
3号
資料タイプ 学術雑誌論文
著者 Fujita, Osamu| Asanuma, Masato| Yokoyama, Teruhiko| Miyazaki, Ikuko| Ogawa, Norio| Kumon, Hiromi|
発行日 2006-12
出版物タイトル Acta Medica Okayama
60巻
6号
資料タイプ 学術雑誌論文
著者 宮崎 育子| 浅沼 幹人| Francisco J. Diaz-Corrales| 三好 耕| 小川 紀雄|
発行日 2008-01-04
出版物タイトル 岡山医学会雑誌
119巻
3号
資料タイプ 学術雑誌論文
著者 淺沼 幹人|
発行日 1992-03-31
出版物タイトル
資料タイプ 学位論文