ID | 49561 |
フルテキストURL | |
著者 |
Tian, FengFeng
Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Deguchi, Kentaro
Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
ORCID
Kaken ID
publons
Yamashita, Toru
Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Ohta, Yasuyuki
Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Morimoto, Nobutoshi
Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Shang, Jingwei
Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Zhang, Xuemei
Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Liu, Ning
Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Ikeda, Yoshio
Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Kaken ID
publons
researchmap
Matsuura, Tohru
Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Kaken ID
publons
researchmap
Abe, Koji
Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Kaken ID
publons
researchmap
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抄録 | Recent studies have suggested that autophagy is involved in a neural death pathway following cerebral ischemia. In vivo detection of autophagy could be important for evaluating ischemic neural cell damage for human stroke patients. Using novel green fluorescent protein (GFP)-fused microtubule-associated protein 1 light chain 3 (LC3) transgenic (Tg) mice, in vivo imaging of autophagy was performed at 1, 3 and 6 d after 60 min transient middle cerebral artery occlusion (tMCAO). Ex vivo imaging of autophagy, testing of the autophagy inhibitor 3-methyladenine (3-MA), estern blot analysis, immunohistochemistry, terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) and fluorescent analyses were performed on brain sections following tMCAO. In vivo fluorescent signals were detected above the ischemic hemisphere through the skull bone at 1, 3 and 6 d after tMCAO, with a peak at 1 d. Similar results were obtained with ex vivo fluorescence imaging. western blot analysis revealed maximum LC3-I and LC3-II expression at 1 d after tMCAO and fluorescence immunohistochemistry demonstrated that GFP-LC3-positive cells were primarily neuronal, not astroglial or microglial, cells. The number of GFP-LC3/TUNEL double-positive cells was greater in the peri-ischemic area than in the core. These results provided evidence of in vivo autophagy detection, with a peak at 1 d, in a live animal model following cerebral ischemia. This novel technique could be valuable for monitoring autophagic processes in vivo in live stroke patients, as well as for clarifying the detailed role of autophagy in the ischemic brain, as well as in other neurological diseases.
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キーワード | autophagy
apoptosis
GFP-LC3 Tg mice
in vivo imaging
tMCAO
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発行日 | 2010-11-16
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出版物タイトル |
Autophagy
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巻 | 6巻
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号 | 8号
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開始ページ | 1107
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終了ページ | 1114
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ISSN | 1554-8627
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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論文のバージョン | author
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
オフィシャル URL | http://doi.org/10.4161/auto.6.8.13427
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関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/49128
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