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ID 32086
JaLCDOI
フルテキストURL
著者
Takata, Hidehiko Okayama University
Tomizawa, Kazuhito Okayama University
Matsushita, Masayuki Okayama University
Matsui, Hideki Okayama University Kaken ID publons researchmap
抄録

Protein transduction therapy using poly-arginine peptide can deliver the biologically active proteins. A previous study showed that 11 poly-arginine fused p53 protein (11R-p53) effectively penetrated across the plasma membrane and inhibited the proliferation of oral cancer cells. However, the intracellular half-life of the delivered protein was less than 36 h. Previous studies also showed that 2-methoxyestradiol (2-ME), an endogenous non-toxic estrogenic metabolite, induces the stabilization of the wild-type p53 protein in human cancer cells posttranscriptionally. In the present study, we examined whether 2-ME induced the stabilization of 11R-p53 and had an inhibitory effect on the proliferation of oral cancer cells. The application of 2-ME significantly enhanced the inhibitory effect of 11R-p53 on the proliferation of oral cancer cells. However, 2-ME had no effect on the intracellular half-life of 11R-p53 in oral cancer cells. Of interest is the finding that 2-ME suppressed the transcriptional activity of NFkappaB, which has an important role in tumorigenesis, but did not affect p53 transcriptional activity. These results suggest that 2-ME synergistically enhances the 11R-p53-induced inhibition of the proliferation of oral cancer cells through the suppression of NFkB transcription.

キーワード
tumor
TAT
poly arginine
gene therapy
protein therapy
Amo Type
Article
出版物タイトル
Acta Medica Okayama
発行日
2004-08
58巻
4号
出版者
Okayama University Medical School
開始ページ
181
終了ページ
187
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
英語
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Science KeyUT