ID | 32024 |
JaLCDOI | |
フルテキストURL | |
著者 |
Ono, Toshiaki
Okayama University
Ishikawa, Takaki
Okayama University
Ishizu, Hideo
Okayama University
|
抄録 | We developed a method for human identification of forensic biological materials by PCR-based detection of a human-specific sequence in exon 3 of the myoglobin gene. This human-specific DNA sequence was deduced from differences in the amino acid sequences of myoglobins between humans and other animal species. The new method enabled amplification of the target DNA fragment from 30 samples of human DNA, and the amplified sequences were identical with that already reported. Using this method, we were able to distinguish human samples from those of 21 kinds of animals: the crab-eating monkey, horse, cow, sheep, goat, pig, wild boar, dog, raccoon dog, cat, rabbit, guinea pig, hamster, rat, mouse, whale, chicken, pigeon, turtle, frog, and tuna. However, we were unable to distinguish between human and gorilla samples. This method enabled us to detect the target sequence from 25 pg of human DNA, and the target DNA fragment from blood stored at 37 degrees C for 6 months, and from bloodstains heated at 150 degrees C for 4 h or stored at room temperature for 26 years. Herein we also report a practical application of the method for human identification of a bone fragment.</P> |
キーワード | species identification
myoglobin
polymerase chain reaction
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 2001-06
|
巻 | 55巻
|
号 | 3号
|
出版者 | Okayama University Medical School
|
開始ページ | 175
|
終了ページ | 184
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
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PubMed ID | |
Web of Science KeyUT |