ID | 57930 |
フルテキストURL | |
著者 |
Ogawa, Hirohito
Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
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Hirayama, Haruko
Department of Animal Resources, Advanced Science Research Center, Okayama University
Kaken ID
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Tanaka, Satsuki
Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Yata, Norio
Department of Animal Resources, Advanced Science Research Center, Okayama University
Namba, Hikaru
Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
ORCID
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Yamashita, Nobuko
Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
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Yonemitsu, Kenzo
Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University
Maeda, Ken
Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University
Mominoki, Katsumi
Department of Animal Resources, Advanced Science Research Center, Okayama University
Kaken ID
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Yamada, Masao
Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,
Kaken ID
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抄録 | Hepatitis E virus (HEV) is known to cause zoonotic infections from pigs, wild boars and deer. Domestic pigs have been used as an experimental animal model in medical research and training; however, the risks of HEV infection from pigs during animal experiments are largely unknown. Here, we retrospectively investigated the seroprevalence and detection rates of viral RNA in 73 domestic pigs (average 34.5 kg) introduced into an animal experimental facility in a medical school during 2012-2016. We detected anti-HEV immunoglobulin G antibodies in 24 of 73 plasma samples (32.9%), though none of the samples were positive for viral RNA. Plasma samples of 18 pigs were sequentially monitored and were classified into four patterns: sustained positive (5 pigs), sustained negative (5 pigs), conversion to positive (6 pigs) and conversion to negative (2 pigs). HEV genomes were detected in 2 of 4 liver samples from pigs that were transported from the same farm during 2016-2017. Two viral sequences of the overlapping open reading frame (ORF) 2/3 region (97 bp) were identical and phylogenetically fell into genotype 3. A 459-bp length of the ORF2 region of an amplified fragment from a pig transported in 2017 was clustered with the wbJYG1 isolate (subgenotype 3b) with 91.5% (420/459 bp) nucleotide identity. Based on our results, we suggest that domestic pigs introduced into animal facilities carry a potential risk of HEV infection to researchers, trainees and facility staff. Continuous surveillance and precautions are important to prevent HEV infection in animal facilities.
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キーワード | animal experimental facility
domestic pig
hepatitis E virus
zoonosis
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発行日 | 2019-08-24
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出版物タイトル |
Journal of Veterinary Medical Science
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巻 | 81巻
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号 | 8号
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出版者 | The Japanese Society of Veterinary Science
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開始ページ | 1191
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終了ページ | 1196
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ISSN | 0916-7250
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NCID | AA10796138
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | ©2019 The Japanese Society of Veterinary Science
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論文のバージョン | publisher
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PubMed ID | |
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関連URL | isVersionOf https://doi.org/10.1292/jvms.19-0086
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ライセンス | https://creativecommons.org/licenses/by-nc-nd/4.0/
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Citation | Hirohito OGAWA, Haruko HIRAYAMA, Satsuki TANAKA, Norio YATA, Hikaru NAMBA, Nobuko YAMASHITA, Kenzo YONEMITSU, Ken MAEDA, Katsumi MOMINOKI, Masao YAMADA, Risk assessment for hepatitis E virus infection from domestic pigs introduced into an experimental animal facility in a medical school, Journal of Veterinary Medical Science, 2019, Volume 81, Issue 8, Pages 1191-1196, Released August 24, 2019, [Advance publication] Released July 08, 2019, Online ISSN 1347-7439, Print ISSN 0916-7250, https://doi.org/10.1292/jvms.19-0086
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助成機関名 |
日本学術振興会
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助成番号 | 25930022
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