ID | 55410 |
フルテキストURL | |
著者 |
Yamamoto, Yuki
Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
Kaken ID
researchmap
Kohka, Misa
Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
Kobayashi, Yoshihiko
Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
Woclawek-Potocka, Izabela
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences
Okuda, Kiyoshi
Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
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抄録 | Endothelin (EDN) is a possible regulating factor of oviductal motility, which is important for the transport of gametes and embryo. To clarify the factors that control the secretion of EDN in the bovine oviduct, the expression of EDNs, EDN-converting enzymes (ECEs) and EDN receptors (EDNRs) were investigated. All isoforms of EDN (EDN1-3), ECE (ECE1 and ECE2) and EDNR (EDNRA and EDNRB) were immunolocalised in the epithelial cells of the ampulla and the isthmus. EDNRs were also immunolocalised in smooth-muscle cells. The mRNA expression of EDN2 and ECE2 was higher in cultured ampullary oviductal epithelial cells than in isthmic cells. The expression of EDN1, EDN2 and ECE2 in the ampullary tissue was highest on the day of ovulation. Oestradiol-17β increased EDN2 and ECE1 expression, while progesterone increased only ECE1 expression in cultured ampullary epithelial cells. These results indicate that EDNs are produced by epithelial cells and their target site is smooth-muscle and epithelial cells, and suggest that ovarian steroids are regulators of endothelin synthesis in ampullary oviductal epithelial cells.
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キーワード | endothelin converting enzyme
endothelin receptor
epithelial cell
ovarian steroids
oviductal contraction and relaxation
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備考 | This is an Accepted Manuscript of an article published by CSIRO Publishing
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発行日 | 2014-11
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出版物タイトル |
Reproduction, Fertility and Development
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巻 | 28巻
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号 | 6号
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出版者 | CSIRO Publishing
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開始ページ | 673
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終了ページ | 681
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ISSN | 1031-3613
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NCID | AA10718189
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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論文のバージョン | author
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1071/RD14076
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