ID | 30739 |
JaLCDOI | |
フルテキストURL | |
著者 |
Aiga, Ayako
Okayama University
Asaumi, Koji
Okayama University
Lee, You Jin
Chosun University
Kadota, Hiroaki
Okayama University
Mitani, Shigeru
Okayama University
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抄録 | The localization and expression of neurotrophins and their receptors during distraction osteogenesis was investigated in 72 male rat femurs (11 weeks old) to further clarify the concurrence of cellular and molecular events of new bone formation. After osteotomy, a 7-day lag phase was followed by distraction at the rate of 0.25 mm/12 h for 21 days (distraction phase), and a 7-day consolidation phase. The localization of neurotrophins (NGF, BDNF and NT-3) and their receptors tropomyosinrelated kinases (TRKA, TRKB and TRKC) by immunostaining showed positive staining in bone forming cells in each stage, although the presence and staining intensity varied by cell type and phase. The expressions of NGF, BDNF and NT-3 by real-time polymerase chain reaction (real-time PCR) showed that the peak of the mRNA expression of NGF occurred 10 days after distraction. NT-3 increased during bone extension, but decreased when distraction stopped. In contrast, BDNF continued to increase gradually throughout the distraction and consolidation phases. These findings suggest that neurotrophins and their receptors may play different roles in endochondral and intramembranous ossification in distraction osteogenesis. The tension stress caused by distraction may stimulate the expression of neurotrophins and their receptors, and promote osteogenesis.
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キーワード | neurotrophin
Trk
distraction osteogenesis
mechanical stress
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Amo Type | Article
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出版物タイトル |
Acta Medica Okayama
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発行日 | 2006-10
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巻 | 60巻
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号 | 5号
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出版者 | Okayama University Medical School
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開始ページ | 267
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終了ページ | 277
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ISSN | 0386-300X
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NCID | AA00508441
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資料タイプ |
学術雑誌論文
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言語 |
英語
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論文のバージョン | publisher
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査読 |
有り
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PubMed ID | |
Web of Science KeyUT |