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ID 53011
フルテキストURL
著者
Mazaki, Tetsuro Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg
Shiozaki, Yasuyuki Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg
Yamane, Kentaro Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg
Yoshida, Aki Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg
Nakamura, Mariko Kibi Int Univ Jr Coll, Dept Hlth & Welf Program
Yoshida, Yasuhiro Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biomat
Zhou, Di RIKEN, Nano Med Engn Lab
Kitajima, Takashi RIKEN, Nano Med Engn Lab
Tanaka, Masato Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg
Ito, Yoshihiro RIKEN, Nano Med Engn Lab
Ozaki, Toshifumi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg
Matsukawa, Akihiro Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Pathol & Expt Med
抄録
Osteochondral injuries remain difficult to repair. We developed a novel photo-cross-linkable furfurylamine-conjugated gelatin (gelatin-FA). Gelatin-FA was rapidly cross-linked by visible light with Rose Bengal, a light sensitizer, and was kept gelled for 3 weeks submerged in saline at 37 degrees C. When bone marrow-derived stromal cells (BMSCs) were suspended in gelatin-FA with 0.05% Rose Bengal, approximately 87% of the cells were viable in the hydrogel at 24 h after photo-cross-linking, and the chondrogenic differentiation of BMSCs was maintained for up to 3 weeks. BMP4 fusion protein with a collagen binding domain (CBD) was retained in the hydrogels at higher levels than unmodified BMP4. Gelatin-FA was subsequently employed as a scaffold for BMSCs and CBD-BMP4 in a rabbit osteochondral defect model. In both cases, the defect was repaired with articular cartilage-like tissue and regenerated subchondral bone. This novel, photo-cross-linkable gelatin appears to be a promising scaffold for the treatment of osteochondral injury.
発行日
2014-03-25
出版物タイトル
Scientific Reports
4巻
ISSN
2045-2322
資料タイプ
学術雑誌論文
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/52967
言語
English
著作権者
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
論文のバージョン
publisher
査読
有り
DOI
Web of Sience KeyUT