ID | 32632 |
JaLCDOI | |
フルテキストURL | |
著者 |
Ota, Zensuke
Okayama University
Ota, Kosuke
Okayama University
|
抄録 | We developed a "tissue negative staining method" to observe the molecular-level ultrastructure in situ in any portion of the ultrathin sections routinely prepared for electron microscopy. This method was used in electron microscopy of the glomerular basement membranes (GBM). The GBM in patients with nephrotic syndrome was discovered to possess a tunnel structure, designated as "nephrotic tunnel", with lumen large enough to allow free passage of protein molecules. This tunnel seemed to be involved in the etiology of nephrotic syndrome. This new method appears to be applicable to a variety of purposes in biological studies. |
キーワード | mechanism
proteinuria
nephrotic syndrome
electron microscopy
negative staining
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 1992-12
|
巻 | 46巻
|
号 | 6号
|
出版者 | Okayama University Medical School
|
開始ページ | 483
|
終了ページ | 487
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
PubMed ID | |
Web of Science KeyUT |