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ID 52035
フルテキストURL
著者
Abd Al Kader, Lamia Okayama Univ, Dept Pathol, Grad Sch Med Dent & Pharmaceut Sci
Oka, Takashi Okayama Univ, Dept Pathol, Grad Sch Med Dent & Pharmaceut Sci
Takata, Katsuyoshi Okayama Univ, Dept Pathol, Grad Sch Med Dent & Pharmaceut Sci
Sun, Xu Dalian Med Univ, Dept Pathol, Affiliated Hosp 1
Sato, Hiaki Okayama Univ, Dept Med Technol, Grad Sch Hlth Sci
Murakami, Ichiro Okayama Univ, Dept Pathol, Grad Sch Med Dent & Pharmaceut Sci
Toji, Tomohiro Okayama Univ, Dept Pathol, Grad Sch Med Dent & Pharmaceut Sci
Manabe, Akihiro Okayama Univ, Dept Pathol, Grad Sch Med Dent & Pharmaceut Sci
Kimura, Hiroshi Osaka Univ, Grad Sch Frontier Sci
Yoshino, Tadashi Okayama Univ, Dept Pathol, Grad Sch Med Dent & Pharmaceut Sci
抄録
Polycomb group (PcG) proteins are important for the regulation of hematopoiesis by regulating chromatin compaction and silencing genes related to differentiation and cell cycle. Overexpression of enhancer of zeste homologue 2 (Ezh2) and Bmi-1/PCGF4 has been implicated in solid organ cancers, while Mel-18/PCGF2 has been reported as a tumor suppressor. Detailed expression profiles of PcG proteins and their diagnostic significance in malignant lymphomas are still unknown. In this study, we analyzed the expression levels of Ezh2, Bmi-1, Mel-18, and Ki67 in 197 Hodgkin's and non-Hodgkin's lymphoma patient samples and in lymphoma cell lines using immunohistochemistry, fluorescent immunocytochemistry, and Western blotting. Immunohistochemical staining showed that Ezh2 expression was significantly increased in aggressive compared to indolent subtypes of B cell neoplasms (P = 0.000-0.030), while no significant differences in Bmi-1 expression were found between these subtypes. Compared to the normal counterpart, T cell lymphomas showed significant overexpression of Bmi-1 (P = 0.011) and Ezh2 (P = 0.000). The Ki67 labeling index showed a positive correlation with Ezh2 expression in B cell lymphomas (correlation coefficient (Co) = 0.983, P = 0.000) and T/NK cell lymphomas (Co = 0.629, P = 0.000). Fluorescent immunohistochemical staining showed coexpression of Ezh2 and Ki67 in the same tumor cells, indicating that Ezh2 expression correlates with cell proliferation. Both B and T/NK cell neoplasms showed low expression of Mel-18 and high expression of both Bmi-1 and Ezh2. In conclusion, in aggressive lymphoma variants, Ezh2 is strongly expressed and polycomb repressive complex PRC1.4 dominates over PRC1.2. Coexpression of Bmi-1 and Ezh2 is a characteristic of aggressive lymphomas. Ezh2 correlates with the proliferation and aggressive nature of non-Hodgkin's lymphomas.
キーワード
Ezh2
Bmi-1
Mel-18
Malignant lymphoma
PRC1.2
PRC1.4
発行日
2013-11
出版物タイトル
Virchows Archiv
463巻
5号
開始ページ
697
終了ページ
711
ISSN
0945-6317
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1007/s00428-013-1428-y
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/51966
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
author
査読
有り
DOI
Web of Sience KeyUT