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ID 52269
フルテキストURL
著者
Sato, Kazuhiro Okayama Univ, Bioresources Res Inst Kaken ID
Shin-I, Tadasu Natl Inst Genet
Seki, Motoaki RIKEN, Plant Sci Ctr
Shinozaki, Kazuo RIKEN, Plant Sci Ctr
Yoshida, Hideya Okayama Univ, Bioresources Res Inst
Takeda, Kazuyoshi Okayama Univ, Bioresources Res Inst
Yamazaki, Yukiko Natl Inst Genet
Conte, Matthieu Int Rice Res Inst, Crop Res Informat Lab
Kohara, Yuji Natl Inst Genet
抄録
A collection of 5006 full-length (FL) cDNA sequences was developed in barley. Fifteen mRNA samples from various organs and treatments were pooled to develop a cDNA library using the CAP trapper method. More than 60% of the clones were confirmed to have complete coding sequences, based on comparison with rice amino acid and UniProt sequences. Blastn homologies (E < 1E-5) to rice genes and Arabidopsis genes were 89 and 47%, respectively. Of the 5028 possible amino acid sequences derived from the 5006 FLcDNAs, 4032 (80.2%) were classified into 1678 GreenPhyl multigenic families. There were 555 cDNAs showing low homology to both rice and Arabidopsis. Gene ontology annotation by InterProScan indicated that many of these cDNAs (71%) have no known molecular functions and may be unique to barley. The cDNAs showed high homology to Barley 1 GeneChip oligo probes (81%) and the wheat gene index (84%). The high homology between FLcDNAs (27%) and mapped barley expressed sequence tag enabled assigning linkage map positions to 151-233 FLcDNAs on each of the seven barley chromosomes. These comprehensive barley FLcDNAs provide strong platform to connect preexisting genomic and genetic resources and accelerate gene identification and genome analysis in barley and related species.
キーワード
full-length cDNA
Hordeum vulgare
mRNA
gene ontology
発行日
2009-04
出版物タイトル
DNA Research
16巻
2号
出版者
Oxford University Press
開始ページ
81
終了ページ
89
ISSN
1340-2838
資料タイプ
学術雑誌論文
言語
English
著作権者
© The Author 2009. Kazusa DNA Research Institute.
論文のバージョン
publisher
査読
有り
DOI
Web of Science KeyUT