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ID 55280
フルテキストURL
著者
Hisano, Hiroshi Institute of Plant Science and Resources, Okayama University
Meints, Brigid Department Crop and Soil Sciences, Washington State University
Moscou, Matthew J. The Sainsbury Laboratory
Cistue, Luis Department Genetica y Produccion Vegetal
Echávarri, Begoña
Sato, Kazuhiro Institute of Plant Science and Resources, Okayama University ORCID Kaken ID publons researchmap
Hayes, Patrick M. Department Crop and Soil Science, Oregon State University
抄録
Key message: The genetic substitution of transformation amenability alleles from ‘Golden Promise’ can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars. Abstract: Barley (Hordeum vulgare) cv. ‘Golden Promise’ is one of the most useful and well-studied cultivars for genetic manipulation. In a previous report, we identified several transformation amenability (TFA) loci responsible for Agrobacterium-mediated transformation using the F2 generation of immature embryos, derived from ‘Haruna Nijo’ × ‘Golden Promise,’ as explants. In this report, we describe higher density mapping of these TFA regions with additional SNP markers using the same transgenic plants. To demonstrate the robustness of transformability alleles at the TFA loci, we genotyped 202 doubled haploid progeny from the cross ‘Golden Promise’ × ‘Full Pint.’ Based on SNP genotype, we selected lines having ‘Golden Promise’ alleles at TFA loci and used them for transformation. Of the successfully transformed lines, DH120366 came the closest to achieving a level of transformation efficiency comparable to ‘Golden Promise.’ The results validate that the genetic substitution of TFA alleles from ‘Golden Promise’ can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars.
キーワード
Agrobacterium tumefaciens
Doubled haploid
Hordeum vulgare (barley)
Single nucleotide polymorphism
Transformation
発行日
2017-04
出版物タイトル
Plant Cell Reports
36巻
4号
出版者
Springer
開始ページ
611
終了ページ
620
ISSN
0721-7714
NCID
AA10635864
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン
author
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1007/s00299-017-2107-2