ID | 52742 |
フルテキストURL | |
著者 |
Takahara, Masahiro
Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
Nemoto, Yasuhiro
Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
Oshima, Shigeru
Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
Matsuzawa, Yu
Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
Kanai, Takanori
Keio Univ, Dept Internal Med, Div Gastroenterol & Hepatol, Sch Med
Okamoto, Ryuichi
Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
Tsuchiya, Kiichiro
Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
Nakamura, Tetsuya
Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
Yamamoto, Kazuhide
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Gastroenterol & Hepatol
ORCID
Kaken ID
publons
Watanabe, Mamoru
Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
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抄録 | Colitogenic memory CD4(+) T cells are important in the pathogenesis of inflammatory bowel disease (IBD). Although memory stem cells with high survival and self-renewal capacity were recently identified in both mice and humans, it is unclear whether a similar subset is present in chronic colitis mice. We sought to identify and purify a long-lived subset of colitogenic memory CD4(+) T cells, which may be targets for treatment of IBD. A long-lived subset of colitogenic memory CD4(+) T cells was purified using a long-term culture system. The characteristics of these cells were assessed. Interleukin (IL)-7 promoted the in vitro survival for >8 weeks of lamina propria (LP) CD4(+) T cells from colitic SOD mice previously injected with CD4(+)CD45RB(high) T cells. These cells were in a quiescent state and divided a maximum of 5 times in 4 weeks. LP CD4(+) T cells expressed higher levels of Bcl-2, integrin-alpha 4 beta 7, CXCR3 and CD25 after than before culture, as well as secreting high concentrations of IL-2 and low concentrations of IFN-gamma and IL-17 in response to intestinal bacterial antigens. LP CD4(+) T cells from colitic mice cultured with IL-7 for 8 weeks induced more severe colitis than LP CD4(+) T cells cultured for 4 weeks. We developed a novel culture system to purify a long-lived, highly pathogenic memory subset from activated LP CD4(+) T cells. IL-7 promoted long-term in vitro survival of this subset in a quiescent state. This subset will be a novel, effective target for the treatment of IBD.
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キーワード | Inflammatory bowel disease
Memory T cells
CD4
IL-7
Cell culture
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発行日 | 2013-12
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出版物タイトル |
Immunology Letters
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巻 | 156巻
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号 | 1-2号
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出版者 | Elsevier Science BV.
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開始ページ | 82
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終了ページ | 93
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ISSN | 0165-2478
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NCID | AA00231960
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資料タイプ |
学術雑誌論文
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関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/52510
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言語 |
英語
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著作権者 | © 2013 Elsevier B.V. All rights reserved.
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論文のバージョン | author
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査読 |
有り
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DOI | |
Web of Science KeyUT |