start-ver=1.4
cd-journal=joma
no-vol=87
cd-vols=
no-issue=11
article-no=
start-page=1323
end-page=1331
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230808
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The effect of exogenous dihydroxyacetone and methylglyoxal on growth, anthocyanin accumulation, and the glyoxalase system in Arabidopsis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Dihydroxyacetone (DHA) occurs in wide-ranging organisms, including plants, and can undergo spontaneous conversion to methylglyoxal (MG). While the toxicity of MG to plants is well-known, the toxicity of DHA to plants remains to be elucidated. We investigated the effects of DHA and MG on Arabidopsis. Exogenous DHA at up to 10 mM did not affect the radicle emergence, the expansion of green cotyledons, the seedling growth, or the activity of glyoxalase II, while DHA at 10 mM inhibited the root elongation and increased the activity of glyoxalase I. Exogenous MG at 1.0 mM inhibited these physiological responses and increased both activities. Dihydroxyacetone at 10 mM increased the MG content in the roots. These results indicate that DHA is not so toxic as MG in Arabidopsis seeds and seedlings and suggest that the toxic effect of DHA at high concentrations is attributed to MG accumulation by the conversion to MG.
en-copyright=
kn-copyright=

en-aut-name=ZhaoMaoxiang
en-aut-sei=Zhao
en-aut-mei=Maoxiang
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NakamuraToshiyuki
en-aut-sei=Nakamura
en-aut-mei=Toshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NakamuraYoshimasa
en-aut-sei=Nakamura
en-aut-mei=Yoshimasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MunemasaShintaro
en-aut-sei=Munemasa
en-aut-mei=Shintaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MoriIzumi C
en-aut-sei=Mori
en-aut-mei=Izumi C
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MurataYoshiyuki
en-aut-sei=Murata
en-aut-mei=Yoshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=4
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=5
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=6
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=dihydroxyacetone
kn-keyword=dihydroxyacetone
en-keyword=methylglyoxal
kn-keyword=methylglyoxal
en-keyword=growth
kn-keyword=growth
en-keyword=anthocyanin
kn-keyword=anthocyanin
en-keyword=glyoxalase system
kn-keyword=glyoxalase system
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=16
article-no=
start-page=4778
end-page=4796
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200506
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Low temperature modulates natural peel degreening in lemon fruit independently of endogenous ethylene
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Peel degreening is an important aspect of fruit ripening in many citrus fruit, and previous studies have shown that it can be advanced by ethylene treatment or by low-temperature storage. However, the important regulators and pathways involved in natural peel degreening remain largely unknown. To determine how natural peel degreening is regulated in lemon fruit (Citrus limon), we studied transcriptome and physiochemical changes in the flavedo in response to ethylene treatment and low temperatures. Treatment with ethylene induced rapid peel degreening, which was strongly inhibited by the ethylene antagonist, 1-methylcyclopropene (1-MCP). Compared with 25 degrees C, moderately low storage temperatures of 5-20 degrees C also triggered peel degreening. Surprisingly, repeated 1-MCP treatments failed to inhibit the peel degreening induced by low temperature. Transcriptome analysis revealed that low temperature and ethylene independently regulated genes associated with chlorophyll degradation, carotenoid metabolism, photosystem proteins, phytohormone biosynthesis and signalling, and transcription factors. Peel degreening of fruit on trees occurred in association with drops in ambient temperature, and it coincided with the differential expression of low temperature-regulated genes. In contrast, genes that were uniquely regulated by ethylene showed no significant expression changes during on-tree peel degreening. Based on these findings, we hypothesize that low temperature plays a prominent role in regulating natural peel degreening independently of ethylene in citrus fruit.
en-copyright=
kn-copyright=

en-aut-name=MitaloOscar W.
en-aut-sei=Mitalo
en-aut-mei=Oscar W.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OtsukiTakumi
en-aut-sei=Otsuki
en-aut-mei=Takumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OkadaRui
en-aut-sei=Okada
en-aut-mei=Rui
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=ObitsuSaeka
en-aut-sei=Obitsu
en-aut-mei=Saeka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MasudaKanae
en-aut-sei=Masuda
en-aut-mei=Kanae
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HojoYuko
en-aut-sei=Hojo
en-aut-mei=Yuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=MatsuuraTakakazu
en-aut-sei=Matsuura
en-aut-mei=Takakazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=MoriIzumi C.
en-aut-sei=Mori
en-aut-mei=Izumi C.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=AbeDaigo
en-aut-sei=Abe
en-aut-mei=Daigo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=AsicheWilliam O.
en-aut-sei=Asiche
en-aut-mei=William O.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=AkagiTakashi
en-aut-sei=Akagi
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=KuboYasutaka
en-aut-sei=Kubo
en-aut-mei=Yasutaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=UshijimaKoichiro
en-aut-sei=Ushijima
en-aut-mei=Koichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=4
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=5
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=6
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=7
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=8
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=9
en-affil=National Agriculture and Food Research Organization, Shikoku Research Station
kn-affil=

affil-num=10
en-affil=Department of Research and Development, Del Monte Kenya Ltd
kn-affil=

affil-num=11
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=12
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=13
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=1-methylcyclopropene
kn-keyword=1-methylcyclopropene
en-keyword=carotenoids
kn-keyword=carotenoids
en-keyword=chlorophyll
kn-keyword=chlorophyll
en-keyword=Citrus limon
kn-keyword=Citrus limon
en-keyword=ethylene
kn-keyword=ethylene
en-keyword=low temperature
kn-keyword=low temperature
en-keyword=peel degreening
kn-keyword=peel degreening
en-keyword=phytohormones
kn-keyword=phytohormones
en-keyword=transcriptome
kn-keyword=transcriptome
END

start-ver=1.4
cd-journal=joma
no-vol=247
cd-vols=
no-issue=
article-no=
start-page=125933
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200116
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Application of the cellular oxidation biosensor to Toxicity Identification Evaluations for high-throughput toxicity assessment of river water
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Toxicity Identification Evaluation (TIE) is a useful method for the classification and identification of toxicants in a composite environment water sample. However, its extension to a larger sample size has been restrained owing to the limited throughput of toxicity bioassays. Here we reported the development of a high-throughput method of TIE Phase I. This newly developed method was assisted by the fluorescence-based cellular oxidation (CO) biosensor fabricated with roGFP2-expressing bacterial cells in 96-well microplate format. The assessment of four river water samples from Langat river basin by this new method demonstrated that the contaminant composition of the four samples can be classified into two distinct groups. The entire toxicity assay consisted of 2338 tests was completed within 12 h with a fluorescence microplate reader. Concurrently, the sample volume for each assay was reduced to 50 ƒŹL, which is 600 to 4700 times lesser to compare with conventional bioassays. These imply that the throughput of the CO biosensor-assisted TIE Phase I is now feasible for constructing a large-scale toxicity monitoring system, which would cover a whole watershed scale.
en-copyright=
kn-copyright=

en-aut-name=OoiLia
en-aut-sei=Ooi
en-aut-mei=Lia
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OkazakiKeisuke
en-aut-sei=Okazaki
en-aut-mei=Keisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=Arias-BarreiroCarlos R.
en-aut-sei=Arias-Barreiro
en-aut-mei=Carlos R.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HengLee Yook
en-aut-sei=Heng
en-aut-mei=Lee Yook
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MoriIzumi C.
en-aut-sei=Mori
en-aut-mei=Izumi C.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil= Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=2
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=3
en-affil=nstitute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=4
en-affil=Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), Institute for Environment and Development (LESTARI), The National University of Malaysia
kn-affil=

affil-num=5
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=
en-keyword=High-throughput cytotoxicity biosensor
kn-keyword=High-throughput cytotoxicity biosensor
en-keyword=Toxicity identification evaluation
kn-keyword=Toxicity identification evaluation
en-keyword=River water pollution
kn-keyword=River water pollution
en-keyword=Ecotoxicity management
kn-keyword=Ecotoxicity management
en-keyword=Integrated watershed management
kn-keyword=Integrated watershed management
END

start-ver=1.4
cd-journal=joma
no-vol=42
cd-vols=
no-issue=2
article-no=
start-page=437
end-page=447
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=20180716
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The mechanism of SO2 -induced stomatal closure differs from O3 and CO2 responses and is mediated by nonapoptotic cell death in guard cells.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Plants closing stomata in the presence of harmful gases is believed to be a stress avoidance mechanism. SO2 , one of the major airborne pollutants, has long been reported to induce stomatal closure, yet the mechanism remains unknown. Little is known about the stomatal response to airborne pollutants besides O3 . SLOW ANION CHANNEL-ASSOCIATED 1 (SLAC1) and OPEN STOMATA 1 (OST1) were identified as genes mediating O3 -induced closure. SLAC1 and OST1 are also known to mediate stomatal closure in response to CO2 , together with RESPIRATORY BURST OXIDASE HOMOLOGs (RBOHs). The overlaying roles of these genes in response to O3 and CO2 suggested that plants share their molecular regulators for airborne stimuli. Here, we investigated and compared stomatal closure event induced by a wide concentration range of SO2 in Arabidopsis through molecular genetic approaches. O3 - and CO2 -insensitive stomata mutants did not show significant differences from the wild type in stomatal sensitivity, guard cell viability, and chlorophyll content revealing that SO2 -induced closure is not regulated by the same molecular mechanisms as for O3 and CO2 . Nonapoptotic cell death is shown as the reason for SO2 -induced closure, which proposed the closure as a physicochemical process resulted from SO2 distress, instead of a biological protection mechanism.
en-copyright=
kn-copyright=

en-aut-name=Ooi Lia
en-aut-sei=Ooi 
en-aut-mei=Lia
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MatsuuraTakakazu
en-aut-sei=Matsuura
en-aut-mei=Takakazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MunemasaShintaro
en-aut-sei=Munemasa
en-aut-mei=Shintaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MurataYoshiyuki
en-aut-sei=Murata
en-aut-mei=Yoshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KatsuharaMaki
en-aut-sei=Katsuhara
en-aut-mei=Maki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HirayamaTakashi
en-aut-sei=Hirayama
en-aut-mei=Takashi
kn-aut-name=•½ŽRūéŽu
kn-aut-sei=•½ŽR
kn-aut-mei=ūéŽu
aut-affil-num=6
ORCID=

en-aut-name=MoriIzumi C.
en-aut-sei=Mori
en-aut-mei=Izumi C.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil= Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=2
en-affil= Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=4
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=5
en-affil= Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=6
en-affil= Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=7
en-affil= Institute of Plant Science and Resources, Okayama University
kn-affil=
en-keyword=airborne pollutants
kn-keyword=airborne pollutants
en-keyword=nonapoptotic cell death
kn-keyword=nonapoptotic cell death
en-keyword=stomatal closure
kn-keyword=stomatal closure
en-keyword=sulfur dioxide
kn-keyword=sulfur dioxide
END

start-ver=1.4
cd-journal=joma
no-vol=57
cd-vols=
no-issue=8
article-no=
start-page=1779
end-page=1990
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160801
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Involvement of OST1 Protein Kinase and PYR/PYL/RCAR Receptors in Methyl Jasmonate-Induced Stomatal Closure in Arabidopsis Guard Cells
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Methyl jasmonate (MeJA) induces stomatal closure. It has been shown that stomata of many ABA-insensitive mutants are also insensitive to MeJA, and a low amount of ABA is a prerequisite for the MeJA response. However, the molecular mechanisms of the interaction between ABA and MeJA signaling remain to be elucidated. Here we studied the interplay of signaling of the two hormones in guard cells using the quadruple ABA receptor mutant pyr1 pyl1 pyl2 pyl4 and ABA-activated protein kinase mutants ost1-2 and srk2e. In the quadruple mutant, MeJA-induced stomatal closure, H2O2 production, nitric oxide (NO) production, cytosolic alkalization and plasma membrane Ca(2+)-permeable current (ICa) activation were not impaired. At the same time, the inactivation of the inward-rectifying K(+) current was impaired. In contrast to the quadruple mutant, MeJA-induced stomatal closure, H2O2 production, NO production and cytosolic alkalization were impaired in ost1-2 and srk2e as well as in aba2-2, the ABA-deficient mutant. The activation of ICa was also impaired in srk2e. Collectively, these results indicated that OST1 was essential for MeJA-induced stomatal closure, while PYR1, PYL1, PYL2 and PYL4 ABA receptors were not sufficient factors. MeJA did not appear to activate OST1 kinase activity. This implies that OST1 mediates MeJA signaling through an undetectable level of activity or a non-enzymatic action. MeJA induced the expression of an ABA synthesis gene, NCED3, and increased ABA contents only modestly. Taken together with previous reports, this study suggests that MeJA signaling in guard cells is primed by ABA and is not brought about through the pathway mediated by PYR1, PYL1 PYL2 and PYL4.
en-copyright=
kn-copyright=

en-aut-name=YinYe
en-aut-sei=Yin
en-aut-mei=Ye
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=AdachiYuji
en-aut-sei=Adachi
en-aut-mei=Yuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NakamuraYoshimasa
en-aut-sei=Nakamura
en-aut-mei=Yoshimasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MunemasaShintaro
en-aut-sei=Munemasa
en-aut-mei=Shintaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MoriIzumi C.
en-aut-sei=Mori
en-aut-mei=Izumi C.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MurataYoshiyuki
en-aut-sei=Murata
en-aut-mei=Yoshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=4
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=5
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=6
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=ABA
kn-keyword=ABA
en-keyword=ABA receptors
kn-keyword=ABA receptors
en-keyword=Arabidopsis thaliana
kn-keyword=Arabidopsis thaliana
en-keyword=Guard cells
kn-keyword=Guard cells
en-keyword=Methyl jasmonate
kn-keyword=Methyl jasmonate
en-keyword=OST1 protein kinase
kn-keyword=OST1 protein kinase
END

start-ver=1.4
cd-journal=joma
no-vol=120
cd-vols=
no-issue=
article-no=
start-page=299
end-page=304
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=201502
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Toxicity of tetramethylammonium hydroxide to aquatic organisms and its synergistic action with potassium iodide
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The aquatic ecotoxicity of chemicals involved in the manufacturing process of thin film transistor liquid crystal displays was assessed with a battery of four selected acute toxicity bioassays. We focused on tetramethylammonium hydroxide (TMAH, CAS No. 75-59-2), a widely utilized etchant. The toxicity of TMAH was low when tested in the 72 h-algal growth inhibition test (Pseudokirchneriellia subcapitata, EC50 = 360 mg L?1) and the Microtox? test (Vibrio fischeri, IC50 = 6.4 g L?1). In contrast, the 24 h-microcrustacean immobilization and the 96 h-fish mortality tests showed relatively higher toxicity (Daphnia magna, EC50 = 32 mg L?1 and Oryzias latipes, LC50 = 154 mg L?1). Isobologram and mixture toxicity index analyses revealed apparent synergism of the mixture of TMAH and potassium iodide when examined with the D. magna immobilization test. The synergistic action was unique to iodide over other halide salts i.e. fluoride, chloride and bromide. Quaternary ammonium ions with longer alkyl chains such as tetraethylammonium and tetrabutylammonium were more toxic than TMAH in the D. magna immobilization test.
en-copyright=
kn-copyright=

en-aut-name=MoriIzumi C.
en-aut-sei=Mori
en-aut-mei=Izumi C.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=Arias-BarreiroCarlos R.
en-aut-sei=Arias-Barreiro
en-aut-mei=Carlos R.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KoutsaftisApostolos
en-aut-sei=Koutsaftis
en-aut-mei=Apostolos
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OgoAtsushi
en-aut-sei=Ogo
en-aut-mei=Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KawanoTomonori
en-aut-sei=Kawano
en-aut-mei=Tomonori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=YoshizukaKazuharu
en-aut-sei=Yoshizuka
en-aut-mei=Kazuharu
kn-aut-name=
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en-aut-name=Inayat-HussainSalmaan H.
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en-aut-mei=Salmaan H.
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en-aut-name=AoyamaIsao
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affil-num=1
en-affil=
kn-affil=Institute of Plant Science and Resources, Okayama University

affil-num=2
en-affil=
kn-affil=Institute of Plant Science and Resources, Okayama University

affil-num=3
en-affil=
kn-affil=Institute of Plant Science and Resources, Okayama University

affil-num=4
en-affil=
kn-affil=Institute of Plant Science and Resources, Okayama University

affil-num=5
en-affil=
kn-affil=School of International Environmental Science, The University of Kitakyushu

affil-num=6
en-affil=
kn-affil=School of International Environmental Science, The University of Kitakyushu

affil-num=7
en-affil=
kn-affil=Faculty of Health Sciences, Univerisiti Kebangsaan Malaysia

affil-num=8
en-affil=
kn-affil=Institute of Plant Science and Resources, Okayama University
en-keyword=Tetramethylammonium hydroxide
kn-keyword=Tetramethylammonium hydroxide
en-keyword=Potassium iodide
kn-keyword=Potassium iodide
en-keyword=Aquatic toxicity
kn-keyword=Aquatic toxicity
en-keyword=Synergism
kn-keyword=Synergism
en-keyword=D. magna
kn-keyword=D. magna
en-keyword=Semiconductor wastewater
kn-keyword=Semiconductor wastewater
END

start-ver=1.4
cd-journal=joma
no-vol=31
cd-vols=
no-issue=
article-no=
start-page=21
end-page=25
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=200912
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Cooling effect on buildings by the roof greening at Research Institute for Bioresources, Okayama University
kn-title=‰ŖŽR‘åŠwŽ‘Œ¹¶•Ø‰ČŠwŒ¤‹†Š‚ɂ؂Ƃ鉮ć—Ī‰»‚É‚ę‚錚•Ø—ā‹pŒų‰Ź
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Roof greening is known to be environmentally friendly technology. Recently developed new roof greening systems, such as the thin-layer/Excel soil? system and the wetland type greening system, were tested at the roof top of buildings of Research Institute for Bioresources, Okayama University. After a multi-year test, these new systems have been established during high-temperature and less-rainfall summer seasons in the south Okayama region. Data indicated that roof greening effectively reduced the temperature of the concrete surface (more than 10‹C). The room temperature under the green roof was also reduced both in a stock room (up to 6‹C) and in an office room (about 2‹C). We also provided the estimation indicating that this roof greening is useful for the decrease in CO(2) emission through the reduction of the electric power for air-conditioning in the summer.
en-copyright=
kn-copyright=

en-aut-name=KatsuharaMaki
en-aut-sei=Katsuhara
en-aut-mei=Maki
kn-aut-name=ŠŽŒ“^–Ų
kn-aut-sei=ŠŽŒ“
kn-aut-mei=^–Ų
aut-affil-num=1
ORCID=

en-aut-name=TanakamaruShigemi
en-aut-sei=Tanakamaru
en-aut-mei=Shigemi
kn-aut-name=“c’†ŠŪd”ü
kn-aut-sei=“c’†ŠŪ
kn-aut-mei=d”ü
aut-affil-num=2
ORCID=

en-aut-name=MoriIzumi C.
en-aut-sei=Mori
en-aut-mei=Izumi C.
kn-aut-name=Xņ
kn-aut-sei=X
kn-aut-mei=ņ
aut-affil-num=3
ORCID=

en-aut-name=TaniAkio
en-aut-sei=Tani
en-aut-mei=Akio
kn-aut-name=’J–¾¶
kn-aut-sei=’J
kn-aut-mei=–¾¶
aut-affil-num=4
ORCID=

en-aut-name=UtsugiShigeko
en-aut-sei=Utsugi
en-aut-mei=Shigeko
kn-aut-name=‰F“s–Ų”ÉŽq
kn-aut-sei=‰F“s–Ų
kn-aut-mei=”ÉŽq
aut-affil-num=5
ORCID=

en-aut-name=EnomotoTakashi
en-aut-sei=Enomoto
en-aut-mei=Takashi
kn-aut-name=‰|–{Œh
kn-aut-sei=‰|–{
kn-aut-mei=Œh
aut-affil-num=6
ORCID=

en-aut-name=MaitaniToshihiko
en-aut-sei=Maitani
en-aut-mei=Toshihiko
kn-aut-name=•Ä’Jr•F
kn-aut-sei=•Ä’J
kn-aut-mei=r•F
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=‰ŖŽR‘åŠwŽ‘Œ¹¶•Ø‰ČŠwŒ¤‹†Š

affil-num=2
en-affil=
kn-affil=‰ŖŽR‘åŠwŽ‘Œ¹¶•Ø‰ČŠwŒ¤‹†Š

affil-num=3
en-affil=
kn-affil=‰ŖŽR‘åŠwŽ‘Œ¹¶•Ø‰ČŠwŒ¤‹†Š

affil-num=4
en-affil=
kn-affil=‰ŖŽR‘åŠwŽ‘Œ¹¶•Ø‰ČŠwŒ¤‹†Š

affil-num=5
en-affil=
kn-affil=‰ŖŽR‘åŠwŽ‘Œ¹¶•Ø‰ČŠwŒ¤‹†Š

affil-num=6
en-affil=
kn-affil=‰ŖŽR‘åŠwŽ‘Œ¹¶•Ø‰ČŠwŒ¤‹†Š

affil-num=7
en-affil=
kn-affil=‰ŖŽR‘åŠwŽ‘Œ¹¶•Ø‰ČŠwŒ¤‹†Š
en-keyword=Roof greening
kn-keyword=Roof greening
en-keyword=wetland type greening
kn-keyword=wetland type greening
en-keyword=thin-Iayer/ Excel soil? system
kn-keyword=thin-Iayer/ Excel soil? system
en-keyword=cooling effect
kn-keyword=cooling effect
END