start-ver=1.4
cd-journal=joma
no-vol=61
cd-vols=
no-issue=8
article-no=
start-page=1115
end-page=1123
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220415
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Clinicopathological Characteristics of Superficial Barrett's Adenocarcinoma in a Japanese Population: A Retrospective, Multicenter Study
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Objective Although Barrett's adenocarcinoma (BA) remains a minor disease in Japan, its incidence has been gradually increasing. We analyzed the characteristics of BA in Japanese populations. <br>
Methods We retrospectively reviewed medical records and analyzed the clinicopathological differences between short-segment Barrett's esophagus (SSBE) and long-segment Barrett's esophagus (LSBE), as well as metastasis. Local recurrence and metachronous lesions were analyzed only in patients who underwent endoscopic resection (ER). <br>
Patients Consecutive patients who had pathological T1 BAs resected by ER or surgery from January 2003 <br>
Results A total of 168 patients were analyzed, including 139 with SSBE and 29 with LSBE. In total, 67% of the SSBE lesions and 32% of the LSBE lesions were located between 0 and 3 o'clock (p=0.0014). No patients who achieved pathological margin-free resection (pR0) and 17% of patients who did not achieve pR0 experienced local recurrence (p=0.0131). None of the patients without lymphovascular involvement, a poorly differentiated component, lesion size of >30 mm, and submucosal invasion of >500 mu m experienced metastasis. The 5-year cumulative incidence rate of metachronous BA after ER was 0% in patients with SSBE and 40% in patients with LSBE (p=0.0005). <br>
Conclusion Superficial BA was likely to be detected at the right anterior wall of SSBE in the Japanese population. The risk for metachronous BA after ER was high in Japanese patients with LSBE, as in Western patients.
en-copyright=
kn-copyright=

en-aut-name=HamadaKenta
en-aut-sei=Hamada
en-aut-mei=Kenta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KanzakiHiromitsu
en-aut-sei=Kanzaki
en-aut-mei=Hiromitsu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MiyaharaKoji
en-aut-sei=Miyahara
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NakagawaMasahiro
en-aut-sei=Nakagawa
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MouriHirokazu
en-aut-sei=Mouri
en-aut-mei=Hirokazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MizunoMotowo
en-aut-sei=Mizuno
en-aut-mei=Motowo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TakahashiSakuma
en-aut-sei=Takahashi
en-aut-mei=Sakuma
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=HoriShinichiro
en-aut-sei=Hori
en-aut-mei=Shinichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=NasuJunichiro
en-aut-sei=Nasu
en-aut-mei=Junichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=TsuzukiTakao
en-aut-sei=Tsuzuki
en-aut-mei=Takao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=MiyaikeJiro
en-aut-sei=Miyaike
en-aut-mei=Jiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=TakenakaRyuta
en-aut-sei=Takenaka
en-aut-mei=Ryuta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=YamauchiKenji
en-aut-sei=Yamauchi
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=KobayashiSayo
en-aut-sei=Kobayashi
en-aut-mei=Sayo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=ToyokawaTatsuya
en-aut-sei=Toyokawa
en-aut-mei=Tatsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=InoueMasafumi
en-aut-sei=Inoue
en-aut-mei=Masafumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=NishimuraMamoru
en-aut-sei=Nishimura
en-aut-mei=Mamoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=MatsubaraMinoru
en-aut-sei=Matsubara
en-aut-mei=Minoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

en-aut-name=TomodaJun
en-aut-sei=Tomoda
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=19
ORCID=

en-aut-name=YamasakiYasushi
en-aut-sei=Yamasaki
en-aut-mei=Yasushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=20
ORCID=

en-aut-name=TanakaTakehiro
en-aut-sei=Tanaka
en-aut-mei=Takehiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=21
ORCID=

en-aut-name=ShirakawaYasuhiro
en-aut-sei=Shirakawa
en-aut-mei=Yasuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=22
ORCID=

en-aut-name=KawaharaYoshiro
en-aut-sei=Kawahara
en-aut-mei=Yoshiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=23
ORCID=

en-aut-name=FujiwaraToshiyoshi
en-aut-sei=Fujiwara
en-aut-mei=Toshiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=24
ORCID=

en-aut-name=OkadaHiroyuki
en-aut-sei=Okada
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=25
ORCID=

en-aut-name=Okayama Gut Study Group
en-aut-sei=Okayama Gut Study Group
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=26
ORCID=

affil-num=1
en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Internal Medicine, Hiroshima City Hospital
kn-affil=

affil-num=4
en-affil=Department of Internal Medicine, Hiroshima City Hospital
kn-affil=

affil-num=5
en-affil=Department of Gastroenterology and Hepatology, Kurashiki Central Hospital
kn-affil=

affil-num=6
en-affil=Department of Gastroenterology and Hepatology, Kurashiki Central Hospital
kn-affil=

affil-num=7
en-affil=Department of Gastroenterology, Kagawa Prefectural Central Hospital
kn-affil=

affil-num=8
en-affil=Department of Endoscopy, National Hospital Organization Shikoku Cancer Center
kn-affil=

affil-num=9
en-affil=Department of Internal Medicine, Okayama Saiseikai General Hospital
kn-affil=

affil-num=10
en-affil=Department of Internal Medicine, Japanese Red Cross Society Himeji Hospital
kn-affil=

affil-num=11
en-affil=Department of Internal Medicine, Saiseikai Imabari Hospital
kn-affil=

affil-num=12
en-affil=Department of Internal Medicine, Tsuyama Chuo Hospital
kn-affil=

affil-num=13
en-affil=Department of Gastroenterology, Mitoyo General Hospital
kn-affil=

affil-num=14
en-affil=Department of Internal Medicine, Fukuyama City Hospital
kn-affil=

affil-num=15
en-affil=Department of Gastroenterology, National Hospital Organization Fukuyama Medical Center
kn-affil=

affil-num=16
en-affil=Department of Gastroenterology, Japanese Red Cross Okayama Hospital
kn-affil=

affil-num=17
en-affil=Department of Internal Medicine, Okayama City Hospital
kn-affil=

affil-num=18
en-affil=Department of Internal Medicine, Sumitomo Besshi Hospital
kn-affil=

affil-num=19
en-affil=Department of Internal Medicine, Akaiwa Medical Association Hospital
kn-affil=

affil-num=20
en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=21
en-affil=Department of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=22
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=23
en-affil=Department of Practical Gastrointestinal Endoscopy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=24
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=25
en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=26
en-affil=
kn-affil=
en-keyword=Barrett's adenocarcinoma
kn-keyword=Barrett's adenocarcinoma
en-keyword=endoscopic resection
kn-keyword=endoscopic resection
en-keyword=long -segment Barrett's esophagus
kn-keyword=long -segment Barrett's esophagus
en-keyword=metachronous
kn-keyword=metachronous
en-keyword=lesion
kn-keyword=lesion
en-keyword=short -segment Barrett's esophagus
kn-keyword=short -segment Barrett's esophagus
en-keyword=surgery
kn-keyword=surgery
END

start-ver=1.4
cd-journal=joma
no-vol=27
cd-vols=
no-issue=10
article-no=
start-page=1602
end-page=1608
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=201210
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Des-gamma-carboxyl prothrombin is associated with tumor angiogenesis in hepatocellular carcinoma
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background and Aim: Hepatocellular carcinoma (HCC) is a hypervascular tumor, and angiogenesis plays an important role in its development. Previously, we demonstrated that des-gamma-carboxyl prothrombin (DCP) promotes both cell proliferation and migration of human umbilical vein endothelial cells (HUVECs) by inducing the autophosphorylation of kinase insert domain receptor (KDR). In the present study, DCP-associated tumor angiogenesis was assessed by comparing hypovascular and common hypervascular HCC. Methods: The solitary HCCs of 827 patients were classified into two groups according to the tumor density at the arterial phase of a dynamic computed tomography scan; the initial clinical data of patients with the hyper- and hypovascular types were compared. The HCC tissues from 95 tumors were analyzed by immunohistochemical staining for DCP and phosphorylated KDR, and intratumoral microvessel density (MVD) was analyzed to evaluate microvessel angiogenesis. Results: The serum DCP levels (320 +/- 3532 mAU/mL) and tumor size (18.4 +/- 9.0 mm) of patients with hypervascular HCC were significantly greater than those with hypovascular HCC (38.7 +/- 80 mAU/mL and 14.6 +/- 5.2 mm, P < 0.001). Immunohistochemical analysis revealed that the expressions of DCP and phospho-KDR were significantly greater in hypervascular HCC (71.4% and 31.0%, respectively) than in hypovascular HCC (7.6% and 5.7%, respectively). Intratumoral MVD was significantly correlated with DCP (r = 0.48, P < 0.0001). Conclusions: des-gamma-carboxyl prothrombin production is associated with tumor angiogenesis in HCC.
en-copyright=
kn-copyright=

en-aut-name=MatsubaraMinoru
en-aut-sei=Matsubara
en-aut-mei=Minoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ShirahaHidenori
en-aut-sei=Shiraha
en-aut-mei=Hidenori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KataokaJyunro
en-aut-sei=Kataoka
en-aut-mei=Jyunro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=IwamuroMasaya
en-aut-sei=Iwamuro
en-aut-mei=Masaya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=HoriguchiShigeru
en-aut-sei=Horiguchi
en-aut-mei=Shigeru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=NishinaShin-ichi
en-aut-sei=Nishina
en-aut-mei=Shin-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TakaokaNobuyuki
en-aut-sei=Takaoka
en-aut-mei=Nobuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=UemuraMasayuki
en-aut-sei=Uemura
en-aut-mei=Masayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=TakakiAkinobu
en-aut-sei=Takaki
en-aut-mei=Akinobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=NakamuraShinichiro
en-aut-sei=Nakamura
en-aut-mei=Shinichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=KobayashiYoshiyuki
en-aut-sei=Kobayashi
en-aut-mei=Yoshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=NousoKazuhiro
en-aut-sei=Nouso
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=YamamotoKazuhide
en-aut-sei=Yamamoto
en-aut-mei=Kazuhide
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama Univ Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=2
en-affil=
kn-affil=Okayama Univ Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=3
en-affil=
kn-affil=Okayama Univ Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=4
en-affil=
kn-affil=Okayama Univ Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=5
en-affil=
kn-affil=Okayama Univ Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=6
en-affil=
kn-affil=Okayama Univ Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=7
en-affil=
kn-affil=Okayama Univ Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=8
en-affil=
kn-affil=Okayama Univ Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=9
en-affil=
kn-affil=Okayama Univ Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=10
en-affil=
kn-affil=Okayama Univ Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=11
en-affil=
kn-affil=Okayama Univ Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=12
en-affil=
kn-affil=Okayama Univ Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=13
en-affil=
kn-affil=Okayama Univ Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
en-keyword=des-gamma-carboxyl prothrombin
kn-keyword=des-gamma-carboxyl prothrombin
en-keyword=hepatocellular carcinoma
kn-keyword=hepatocellular carcinoma
en-keyword=intratumoral microvessel density
kn-keyword=intratumoral microvessel density
en-keyword=kinase insert domain receptor
kn-keyword=kinase insert domain receptor
END

start-ver=1.4
cd-journal=joma
no-vol=131
cd-vols=
no-issue=11
article-no=
start-page=2537
end-page=2546
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20121201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Runt-related transcription factor 3 reverses epithelial-mesenchymal transition in hepatocellular carcinoma
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Loss or decreased expression of runt-related transcription factor 3 (RUNX3), a tumor suppressor gene involved in gastric and other cancers, has been frequently observed in hepatocellular carcinoma (HCC). The objective of this study was to identify the regulatory mechanism of the epithelialmesenchymal transition (EMT) by RUNX3 in HCC. Human HCC cell lines, Hep3B, Huh7, HLF and SK-Hep1, were divided into low- and high-EMT lines, based on their expression of TWIST1 and SNAI2, and were used in this in vitro study. Ectopic RUNX3 expression had an anti-EMT effect in low-EMT HCC cell lines characterized by increased E-cadherin expression and decreased N-cadherin and vimentin expression. RUNX3 expression has previously been reported to reduce jagged-1 (JAG1) expression; therefore, JAG1 ligand peptide was used to reinduce EMT in RUNX3-expressing low-EMT HCC cells. Immunohistochemical analyses were performed for RUNX3, E-cadherin, N-cadherin and TWIST1 in 33 human HCC tissues, also divided into low- and high-EMT HCC, based on TWIST1 expression. E-cadherin expression was correlated positively and N-cadherin expression was correlated negatively with RUNX3 expression in low-EMT HCC tissues. Correlations between EMT markers and RUNX3 mRNA expression were analyzed using Oncomine datasets. Similarly, mRNA expression of E-cadherin was also significantly correlated with that of RUNX3 in low-EMT HCC, while mRNA expression of JAG1 was negatively correlated with that of RUNX3. These results suggest a novel mechanism by which loss or decreased expression of RUNX3 induces EMT via induction of JAG1 expression in low-EMT HCC.
en-copyright=
kn-copyright=

en-aut-name=TanakaShigetomi
en-aut-sei=Tanaka
en-aut-mei=Shigetomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ShirahaHidenori
en-aut-sei=Shiraha
en-aut-mei=Hidenori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NakanishiYutaka
en-aut-sei=Nakanishi
en-aut-mei=Yutaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NishinaShin-Ichi
en-aut-sei=Nishina
en-aut-mei=Shin-Ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MatsubaraMinoru
en-aut-sei=Matsubara
en-aut-mei=Minoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HoriguchiShigeru
en-aut-sei=Horiguchi
en-aut-mei=Shigeru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TakaokaNobuyuki
en-aut-sei=Takaoka
en-aut-mei=Nobuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=IwamuroMasaya
en-aut-sei=Iwamuro
en-aut-mei=Masaya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=KataokaJunro
en-aut-sei=Kataoka
en-aut-mei=Junro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=KuwakiKenji
en-aut-sei=Kuwaki
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=HagiharaHiroaki
en-aut-sei=Hagihara
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=ToshimoriJunichi
en-aut-sei=Toshimori
en-aut-mei=Junichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=OhnishiHideki
en-aut-sei=Ohnishi
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=TakakiAkinobu
en-aut-sei=Takaki
en-aut-mei=Akinobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=NakamuraShinichiro
en-aut-sei=Nakamura
en-aut-mei=Shinichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=NousoKazuhiro
en-aut-sei=Nouso
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=YagiTakahito
en-aut-sei=Yagi
en-aut-mei=Takahito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=YamamotoKazuhide
en-aut-sei=Yamamoto
en-aut-mei=Kazuhide
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=2
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=3
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=4
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=5
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=6
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=7
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=8
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=9
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=10
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=11
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=12
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=13
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=14
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=15
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=16
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol

affil-num=17
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol Surg Transplant & Surg Oncol

affil-num=18
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Gastroenterol & Hepatol
en-keyword=cell migration
kn-keyword=cell migration
en-keyword=tumor invasion
kn-keyword=tumor invasion
en-keyword=jagged-1
kn-keyword=jagged-1
en-keyword=E-cadherin
kn-keyword=E-cadherin
en-keyword=N-cadherin
kn-keyword=N-cadherin
END

start-ver=1.4
cd-journal=joma
no-vol=11
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110104
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Loss of runt-related transcription factor 3 expression leads hepatocellular carcinoma cells to escape apoptosis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background: Runt-related transcription factor 3 (RUNX3) is known as a tumor suppressor gene for gastric cancer and other cancers, this gene may be involved in the development of hepatocellular carcinoma (HCC). 

Methods: RUNX3 expression was analyzed by immunoblot and immunohistochemistry in HCC cells and tissues, respectively. Hep3B cells, lacking endogenous RUNX3, were introduced with RUNX3 constructs. Cell proliferation was measured using the MTT assay and apoptosis was evaluated using DAPI staining. Apoptosis signaling was assessed by immunoblot analysis. 

Results: RUNX3 protein expression was frequently inactivated in the HCC cell lines (91%) and tissues (90%). RUNX3 expression inhibited 90 +/- 8% of cell growth at 72 h in serum starved Hep3B cells. Forty-eight hour serum starvation-induced apoptosis and the percentage of apoptotic cells reached 31 +/- 4% and 4 +/- 1% in RUNX3-expressing Hep3B and control cells, respectively. Apoptotic activity was increased by Bim expression and caspase-3 and caspase-9 activation. 

Conclusion: RUNX3 expression enhanced serum starvation-induced apoptosis in HCC cell lines. RUNX3 is deleted or weakly expressed in HCC, which leads to tumorigenesis by escaping apoptosis.
en-copyright=
kn-copyright=

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kn-affil=Okayama Univ

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kn-affil=Okayama Univ

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kn-affil=Okayama Univ

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kn-affil=Okayama Univ

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kn-affil=Okayama Univ

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kn-affil=Okayama Univ

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kn-affil=Okayama Univ

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kn-affil=Okayama Univ

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kn-affil=Okayama Univ

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kn-affil=Okayama Univ

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kn-affil=Okayama Univ

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kn-affil=Okayama Univ

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kn-affil=Okayama Univ

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kn-affil=Okayama Univ

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kn-affil=Okayama Univ
END