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ID 52795
フルテキストURL
著者
Hirai, Misato Okayama Univ, Grad Sch Med Dent & Pharm, Dept Otolaryngol Head & Neck Surg
Maeda, Yukihide Okayama Univ, Grad Sch Med Dent & Pharm, Dept Otolaryngol Head & Neck Surg Kaken ID publons researchmap
Fukushima, Kunihiro Okayama Univ, Grad Sch Med Dent & Pharm, Dept Otolaryngol Head & Neck Surg
Sugaya, Akiko Okayama Univ, Grad Sch Med Dent & Pharm, Dept Otolaryngol Head & Neck Surg
Kataoka, Yuko Okayama Univ, Grad Sch Med Dent & Pharm, Dept Otolaryngol Head & Neck Surg Kaken ID publons
Nishizaki, Kazunori Okayama Univ, Grad Sch Med Dent & Pharm, Dept Otolaryngol Head & Neck Surg Kaken ID publons researchmap
抄録
MicroRNAs (miRNAs) play functional roles in sound transduction in cochlea. This study focuses on the validity of cochlear culture as an in vitro experimental tool, in view of miRNA expression. E15 cochleae were dissected and maintained in vitro for 48 h before extraction of miRNAs. MiRNA expression was comprehensively screened in explanted cochleae using a miRNA array that covers 380 miRNAs. A strong correlation was observed between expression levels of miRNAs in in vitro and in in vivo cochleae. Levels of 43 miRNAs were altered in vitro and these changes were reproducible over three trials. These findings indicate that in vitro miRNA profiling is a viable method for analysis of gene expression and action of chemical compounds on cochleae.
キーワード
embryonic cochlea
microRNA array
organ culture of cochlea
発行日
2011-09-14
出版物タイトル
NeuroReport
22巻
13号
開始ページ
652
終了ページ
654
ISSN
0959-4965
資料タイプ
学術雑誌論文
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/52518
言語
English
著作権者
(C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
論文のバージョン
author
査読
有り
DOI
Web of Science KeyUT