start-ver=1.4
cd-journal=joma
no-vol=6
cd-vols=
no-issue=7
article-no=
start-page=2300163
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230428
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Particle and Heavy Ion Transport Code System‐Based Microdosimetry for the Development of Boron Agents for Boron Neutron Capture Therapy
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Boron neutron capture therapy (BNCT) is a radiation therapy that selectively kills cancer cells at the cellular level using the boron neutron capture reaction (BNCR) (10B(n.α)7Li). The amount of boron 10B delivers in boronophenylalanine (BPA)-BNCT to achieve anti-tumor effects is ?15?40 ppm. The same is true for all boron drugs; however, whether the same amount of 10B is required for other boron drugs with different accumulation characteristics has not been intensively investigated. Therefore, herein, a virtual cell model with intracellular organelles is prepared, and the BPA equivalent dose concentration to the cell nucleus is analyzed using particle and heavy ion transport code system-based microdosimetry. Additionally, the intranuclear minimal region (IMR) is set as a reference for the concept of the intranuclear domain in the microdosimetric kinetic model, and the BPA equivalent dose concentration to the IMR is estimated. The required boron delivery dose greatly varies depending on the dose assessment based on the accumulation characteristics of boron agents in intracellular organelles. Evaluation of the BNCR effect according to the accumulation characteristics without being influenced by the specified value of 15?40 ppm is recommended.
en-copyright=
kn-copyright=
en-aut-name=ShigehiraTakafumi
en-aut-sei=Shigehira
en-aut-mei=Takafumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=HanafusaTadashi
en-aut-sei=Hanafusa
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=IgawaKazuyo
en-aut-sei=Igawa
en-aut-mei=Kazuyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=KasaiTomonari
en-aut-sei=Kasai
en-aut-mei=Tomonari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=FuruyaShuichi
en-aut-sei=Furuya
en-aut-mei=Shuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=NishimoriHisakazu
en-aut-sei=Nishimori
en-aut-mei=Hisakazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=MaedaYoshinobu
en-aut-sei=Maeda
en-aut-mei=Yoshinobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=MichiueHiroyuki
en-aut-sei=Michiue
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=FujimuraAtsushi
en-aut-sei=Fujimura
en-aut-mei=Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
affil-num=1
en-affil=Department of Cellular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=
affil-num=3
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=
affil-num=4
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=
affil-num=5
en-affil=Research Laboratory of Accelerator-Based BNCT system, Graduate School of Engineering Nagoya University
kn-affil=
affil-num=6
en-affil=Department of Hematology and Oncology Okayama University Hospital Okayama Okayama 700?8558 Japan
kn-affil=
affil-num=7
en-affil=Department of Hematology and Oncology, Okayama University Hospital
kn-affil=
affil-num=8
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=
affil-num=9
en-affil=Department of Cellular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=boron agents
kn-keyword=boron agents
en-keyword=boron neutron capture therapy
kn-keyword=boron neutron capture therapy
en-keyword=simulation study
kn-keyword=simulation study
END
start-ver=1.4
cd-journal=joma
no-vol=9
cd-vols=
no-issue=10
article-no=
start-page=2149
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200923
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=In Vitro Studies to Define the Cell-Surface and Intracellular Targets of Polyarginine-Conjugated Sodium Borocaptate as a Potential Delivery Agent for Boron Neutron Capture Therapy
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Boron neutron capture therapy (BNCT) requires pharmaceutical innovations and molecular-based evidence of effectiveness to become a standard cancer therapeutic in the future. Recently, in Japan, 4-borono-L-phenylalanine (BPA) was approved as a boron agent for BNCT against head and neck (H&N) cancers. H&N cancer appears to be a suitable target for BPA-BNCT, because the expression levels of L-type amino acid transporter 1 (LAT1), one of the amino acid transporters responsible for BPA uptake, are elevated in most cases of H&N cancer. However, in other types of cancer including malignant brain tumors, LAT1 is not always highly expressed. To expand the possibility of BNCT for these cases, we previously developed poly-arginine peptide (polyR)-conjugated mercaptoundecahydrododecaborate (BSH). PolyR confers the cell membrane permeability and tumor selectivity of BSH. However, the molecular determinants for the properties are not fully understood. In this present study, we have identified the cluster of differentiation 44 (CD44) protein and translational machinery proteins as a major cell surface target and intracellular targets of BSH-polyR, respectively. CD44, also known as a stem cell-associated maker in various types of cancer, is required for the cellular uptake of polyR-conjugated molecules. We showed that BSH-polyR was predominantly delivered to a CD44(High) cell population of cancer cells. Once delivered, BSH-polyR interacted with the translational machinery components, including the initiation factors, termination factors, and poly(A)-biding protein (PABP). As a proof of principle, we performed BSH-polyR-based BNCT against glioma stem-like cells and revealed that BSH-polyR successfully induced BNCT-dependent cell death specifically in CD44(High) cells. Bioinformatics analysis indicated that BSH-polyR would be suitable for certain types of malignant tumors. Our results shed light on the biochemical properties of BSH-polyR, which may further contribute to the therapeutic optimization of BSH-BNCT in the future.
en-copyright=
kn-copyright=
en-aut-name=FujimuraAtsushi
en-aut-sei=Fujimura
en-aut-mei=Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=YasuiSeiji
en-aut-sei=Yasui
en-aut-mei=Seiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=IgawaKazuyo
en-aut-sei=Igawa
en-aut-mei=Kazuyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=UedaAi
en-aut-sei=Ueda
en-aut-mei=Ai
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=WatanabeKaori
en-aut-sei=Watanabe
en-aut-mei=Kaori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=HanafusaTadashi
en-aut-sei=Hanafusa
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=IchikawaYasuaki
en-aut-sei=Ichikawa
en-aut-mei=Yasuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=YoshihashiSachiko
en-aut-sei=Yoshihashi
en-aut-mei=Sachiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=TsuchidaKazuki
en-aut-sei=Tsuchida
en-aut-mei=Kazuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=KamiyaAtsunori
en-aut-sei=Kamiya
en-aut-mei=Atsunori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=FuruyaShuichi
en-aut-sei=Furuya
en-aut-mei=Shuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
affil-num=1
en-affil=Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=
affil-num=3
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=
affil-num=4
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=
affil-num=5
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=
affil-num=6
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=
affil-num=7
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=
affil-num=8
en-affil=Graduate School of Engineering, Nagoya University
kn-affil=
affil-num=9
en-affil=Graduate School of Engineering, Nagoya University
kn-affil=
affil-num=10
en-affil=Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=11
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=
en-keyword=boron neutron capture therapy (BNCT)
kn-keyword=boron neutron capture therapy (BNCT)
en-keyword=BSH-polyR
kn-keyword=BSH-polyR
en-keyword=CD44
kn-keyword=CD44
en-keyword=translational machinery
kn-keyword=translational machinery
en-keyword=bioinformatics
kn-keyword=bioinformatics
END
start-ver=1.4
cd-journal=joma
no-vol=48
cd-vols=
no-issue=5
article-no=
start-page=320
end-page=326
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1999
dt-pub=19990515
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Volume Reduction by the Incineration of the Combustible Radioactive Solid Samples from Radioisotope Usage at the Utilization Facility : Estimation of the Distribution of Low Energy β-Emitter Using the Imaging Plate
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We want to establish a system of volume reduction by the incineration of the combustible radioactive solid wastes from radioisotope usage at the utilization facility. We have been performing experiments using an experimental incineration system to examine the distribution of radionuclides during incineration and to collect basic data. To reproduce the realistic conditions of incineration of low-level radioactive wastes in an experimental system, we adopted new incineration methods in this study. Low level radioactive samples (LLRS) were set up in a mesh container of stainless steel and incinerated at high temperature (over 800??) generated by two sets of high calorie gas burners. Low energy ??-emitters 35S, 45Ca, 33P, and a high energy ??-emitter 32P were used for the experiment. Their translocation percentages in exhaust air and dust were estimated using the Imaging Plate. Distribution of radionuclides during the incineration was similar to that estimated by conventional methods by our study or to that reported in incineration of liquid scintillation cocktail waste. We concluded that the use of the Imaging Plates is a simple and reliable method for estimation of the distribution of low energy ??-emitters in incineration gas and ash.
en-copyright=
kn-copyright=
en-aut-name=YumotoYasuhiro
en-aut-sei=Yumoto
en-aut-mei=Yasuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=HanafusaTadashi
en-aut-sei=Hanafusa
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=NagamatsuTomohiro
en-aut-sei=Nagamatsu
en-aut-mei=Tomohiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=OkadaShigeru
en-aut-sei=Okada
en-aut-mei=Shigeru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
affil-num=1
en-affil=Radioisotope Center, Okayama University
kn-affil=
affil-num=2
en-affil=Radioisotope Center, Okayama University
kn-affil=
affil-num=3
en-affil=Radioisotope Center, Okayama University
kn-affil=
affil-num=4
en-affil=Radioisotope Center, Okayama University
kn-affil=
en-keyword=low level radioactive sample (LLRS)
kn-keyword=low level radioactive sample (LLRS)
en-keyword=low energy β-emitter
kn-keyword=low energy β-emitter
en-keyword=Imaging Plate
kn-keyword=Imaging Plate
en-keyword=experimental incineration system
kn-keyword=experimental incineration system
END
start-ver=1.4
cd-journal=joma
no-vol=46
cd-vols=
no-issue=7
article-no=
start-page=443
end-page=449
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1997
dt-pub=1997715
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=低レベル放射性試料の焼却実験システムの設置と燃焼実験
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Setting up Experimental Incineration System for Low-level Radioactive Samples and Combustion Experiments. Yasuhiro YUMOTO, Tadashi HANAFUSA, Tomohiro NAGAMATSU and Shigeru OKADA: Radioisotope Center, Okayama University, 2-5-1, Shikata-cho, Okayama-shi 700, Japan.
An incineration system was constructed which were composed of a combustion furnace (AP-150 R), a cyclone dust collector, radioisotope trapping and measurement apparatus and a radioisotope storage room built in the first basement of the Radioisotope Center.
Low level radioactive samples (LLRS) used for the combustion experiment were composed of combustible material or semi-combustible material containing
500 kBq of 99mTcO4 or 23.25 kBq of 131INa. The distribution of radioisotopes both in the inside and outside of combustion furnace were estimated. We measured radioactivity of a smoke duct gas in terminal exit of the exhaust port. In case of combustion of LLRS containing 99mTcO4 or 131INa, concentration of radioisotopes at the exhaust port showed less than legal concentration limit of these radioisotopes. In cases of combustion of LLRS containing 99mTcO4 or 131INa, decontamination factors of the incineration system were 120 and 1.1, respectively.
en-copyright=
kn-copyright=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=湯本泰弘
kn-aut-sei=湯本
kn-aut-mei=泰弘
aut-affil-num=1
ORCID=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=花房直志
kn-aut-sei=花房
kn-aut-mei=直志
aut-affil-num=2
ORCID=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=永松知洋
kn-aut-sei=永松
kn-aut-mei=知洋
aut-affil-num=3
ORCID=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡田茂
kn-aut-sei=岡田
kn-aut-mei=茂
aut-affil-num=4
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学アイソトープ総合センター
affil-num=2
en-affil=
kn-affil=岡山大学アイソトープ総合センター
affil-num=3
en-affil=
kn-affil=岡山大学アイソトープ総合センター
affil-num=4
en-affil=
kn-affil=岡山大学アイソトープ総合センター
en-keyword=low level radioactive sample (LLRS)
kn-keyword=low level radioactive sample (LLRS)
en-keyword=experimental incineration system for LLRS
kn-keyword=experimental incineration system for LLRS
END
start-ver=1.4
cd-journal=joma
no-vol=313
cd-vols=
no-issue=1
article-no=
start-page=169
end-page=174
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=201707
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=In vitro analysis of radioprotective effect of monoterpenes
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Monoterpenes are naturally occurring hydrocarbons composed of two units of isoprenes. They exhibit antioxidant activity to scavenge reactive oxygen species, such as hydroxyl radicals. We investigated the potential of monoterpenes such as thymol, linalool, and menthol to act as radioprotectants. The proliferation of EL4 cells, a mouse lymphoma cell line, treated with linalool at a concentration of 500 μM or more was not affected by X-ray irradiation. Plasmid-nicking assay performed using formamidopyrimidine-DNA glycosylase showed that linalool prevented single strand breaks and oxidized purines on pUC19 plasmid DNA. These findings indicate that linalool has the ability to scavenge reactive oxygen species and is a potential radioprotector.
en-copyright=
kn-copyright=
en-aut-name=KudoKen-ichi
en-aut-sei=Kudo
en-aut-mei=Ken-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=HanafusaTadashi
en-aut-sei=Hanafusa
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=OnoToshiro
en-aut-sei=Ono
en-aut-mei=Toshiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
affil-num=1
en-affil=
kn-affil=
affil-num=2
en-affil=Department of Radiation Research, Advanced Science Research CenterOkayama University
kn-affil=
affil-num=3
en-affil=Department of Radiation Research, Advanced Science Research CenterOkayama University
kn-affil=
en-keyword=Monoterpenes
kn-keyword=Monoterpenes
en-keyword= Linalool
kn-keyword= Linalool
en-keyword=X-ray irradiation
kn-keyword=X-ray irradiation
en-keyword= Reactive oxygen species
kn-keyword= Reactive oxygen species
en-keyword= SSB
kn-keyword= SSB
END
start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20150620
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Adsorption and removal of strontium in aqueous solution by synthetic hydroxyapatite
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Hydroxyapatite (HAP) is a main mineral constituent of bone and tooth and has an outstanding biocompatibility. HAP is a possible sorbent for heavy metals in wastewater due to its high adsorption capacity and low water solubility. We developed a removal system of 90Sr from aqueous solution by HAP column procedure. More than 90 % of 90Sr was adsorbed and removed from the 90Sr containing solution. Divalent cations, Ca2+, had little effect on the removal of 90Sr up to a concentration of 1 mmol L?1. This clearly indicates that the HAP column technique is advantageous with respect to the capacity to adsorb 90Sr from water present in the environment.
en-copyright=
kn-copyright=
en-aut-name=NishiyamaYuichi
en-aut-sei=Nishiyama
en-aut-mei=Yuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=HanafusaTadashi
en-aut-sei=Hanafusa
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=YamashitaJun
en-aut-sei=Yamashita
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=YamamotoYoko
en-aut-sei=Yamamoto
en-aut-mei=Yoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=OnoToshiro
en-aut-sei=Ono
en-aut-mei=Toshiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
affil-num=1
en-affil=
kn-affil=Department of Radiation Research, Advanced Science Research Center, Okayama University
affil-num=2
en-affil=
kn-affil=Department of Radiation Research, Advanced Science Research Center, Okayama University
affil-num=3
en-affil=
kn-affil=Institute of Plant Science and Resources, Okayama University
affil-num=4
en-affil=
kn-affil=Institute of Plant Science and Resources, Okayama University
affil-num=5
en-affil=
kn-affil=Department of Radiation Research, Advanced Science Research Center, Okayama University
en-keyword=Strontium
kn-keyword=Strontium
en-keyword=Hydroxyapatite
kn-keyword=Hydroxyapatite
en-keyword=Adsorption
kn-keyword=Adsorption
en-keyword=Desorption
kn-keyword=Desorption
en-keyword=Divalent cation
kn-keyword=Divalent cation
END
start-ver=1.4
cd-journal=joma
no-vol=301
cd-vols=
no-issue=1
article-no=
start-page=57
end-page=62
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Isolation and characterization of human lung cancer antigens by serological screening with autologous antibodies
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Serological analysis of a recombinant cDNA expression library (SEREX) derived from two lung adenocarcinoma cancer cell lines using autologous sera led to the isolation of 41 positive cDNA clones comprising 28 different antigens. They coded for a variety of nuclear and cytoplasmic proteins. Among the antigens, nucleoporin 107 (NUP107) was isolated most frequently (5 of 41 clones). The second most frequently isolated antigen was coded for by C21orf58 (4 of 41 clones). During serological analysis of selected antigens based on their reactivity to sera from normal individuals and lung cancer patients, none of the antigens showed a cancer-restricted recognition pattern. However, five genes including NUP107 showed higher expression when we examined the changes in gene expression in five different adenocarcinoma cell lines, including those used in SEREX, compared with their levels in normal lung tissues by cDNA microarray analysis. On the other hand, the expression levels of five genes including C21orf58 were down regulated in all adenocarcinoma cell lines. This SEREX study combining comprehensive gene expression assays has added to the growing list of lung cancer antigens, which may aid the development of diagnostic and immunotherapeutic reagents for patients with lung cancer.
en-copyright=
kn-copyright=
en-aut-name=HanafusaTadashi
en-aut-sei=Hanafusa
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=MohamedAli Eldib Ali
en-aut-sei=Mohamed
en-aut-mei=Ali Eldib Ali
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=KitaokaKenta
en-aut-sei=Kitaoka
en-aut-mei=Kenta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=OhueYoshihiro
en-aut-sei=Ohue
en-aut-mei=Yoshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=NakayamaEiichi
en-aut-sei=Nakayama
en-aut-mei=Eiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=OnoToshiro
en-aut-sei=Ono
en-aut-mei=Toshiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
affil-num=1
en-affil=
kn-affil=Department of Radiation Research, Advanced Science Research Center, Okayama University
affil-num=2
en-affil=
kn-affil=Faculty of Science, Alexandria University
affil-num=3
en-affil=
kn-affil=Hyogo Prefectural Awaji Hospital
affil-num=4
en-affil=
kn-affil=Department of Medicine, Kawasaki Medical University
affil-num=5
en-affil=
kn-affil=Faculty of Health and Welfare, Kawasaki University of Medical Welfare
affil-num=6
en-affil=
kn-affil=Department of Radiation Research, Advanced Science Research Center, Okayama University
en-keyword=Lung adenocarcinoma
kn-keyword=Lung adenocarcinoma
en-keyword=SEREX
kn-keyword=SEREX
en-keyword=Tumor antigen
kn-keyword=Tumor antigen
en-keyword=NUP107
kn-keyword=NUP107
en-keyword=MYL6B
kn-keyword=MYL6B
END
start-ver=1.4
cd-journal=joma
no-vol=46
cd-vols=
no-issue=5
article-no=
start-page=367
end-page=370
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1992
dt-pub=199210
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Detection by western blotting of an antibody to the hepatitis C virus E1 envelope protein in sera of patients with chronic liver disease.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We detected an antibody to HCV envelope protein (E1) in sera of patients with HCV-related chronic liver diseases (20 patients with chronic hepatitis and 5 patients with liver cirrhosis) by Western blotting using the fusion protein of E1 envelope protein and beta-galactosidase as an antigen. The antibody to HCV E1 (anti-HCV E1) was detected in 8 (42%) of 19 patients positive for HCV-RNA (16 were positive and 3 were negative for antibody to C100-3) and in 1 (17%) of 6 patients negative for HCV-RNA but positive for antibody to C100-3. HCV-RNA was detected in 8 (89%) of 9 anti-HCV E1 positive sera. The value of alanine aminotransferase was significantly higher in patients positive for anti-HCV E1 than in patients negative for the antibody. Although an antibody to the envelope protein of HCV is suspected to be one of the candidates of virus-neutralizing antibodies, our results suggest this hypothesis appears to be unlikely.
en-copyright=
kn-copyright=
en-aut-name=HadaHajime
en-aut-sei=Hada
en-aut-mei=Hajime
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=KoideNorio
en-aut-sei=Koide
en-aut-mei=Norio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=HanafusaTadashi
en-aut-sei=Hanafusa
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=SakaguchiKosaku
en-aut-sei=Sakaguchi
en-aut-mei=Kosaku
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=ShinjiToshiyuki
en-aut-sei=Shinji
en-aut-mei=Toshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=SasakiShunsuke
en-aut-sei=Sasaki
en-aut-mei=Shunsuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=OkaTakahiko
en-aut-sei=Oka
en-aut-mei=Takahiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=TakayamaNiro
en-aut-sei=Takayama
en-aut-mei=Niro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=YumotoYasuhiro
en-aut-sei=Yumoto
en-aut-mei=Yasuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=TsujiTakao
en-aut-sei=Tsuji
en-aut-mei=Takao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
affil-num=1
en-affil=
kn-affil=Okayama University
affil-num=2
en-affil=
kn-affil=Okayama University
affil-num=3
en-affil=
kn-affil=Okayama Univeristy
affil-num=4
en-affil=
kn-affil=Okayama University
affil-num=5
en-affil=
kn-affil=Okayama University
affil-num=6
en-affil=
kn-affil=Okayama University
affil-num=7
en-affil=
kn-affil=Okayama University
affil-num=8
en-affil=
kn-affil=Okayama University
affil-num=9
en-affil=
kn-affil=Okayama University
affil-num=10
en-affil=
kn-affil=Okayama University
en-keyword=hepatitis C virus
kn-keyword=hepatitis C virus
en-keyword=envelope
kn-keyword=envelope
en-keyword=antibody
kn-keyword=antibody
en-keyword=Western blotting
kn-keyword=Western blotting
END
start-ver=1.4
cd-journal=joma
no-vol=45
cd-vols=
no-issue=5
article-no=
start-page=347
end-page=355
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1991
dt-pub=199110
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Sequence variations in the envelope protein of the hepatitis C virus: comparison with partial cDNA sequence of a new variant virus obtained by the polymerase chain reaction.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=It has been reported that the envelope region located at the 3' portion of the structural protein coding region is one of the most variable regions at both nucleotide and amino acid sequence levels in the hepatitis C virus (HCV) genome. We cloned HCV cDNA fragments of an envelope protein coding region (HCVNK), which were derived from serum of a Japanese patient with hepatocellular carcinoma and were amplified by polymerase chain reaction. After determining the nucleotide sequence, deduced amino acid sequence of the envelope protein region was compared with those of six HCV strains already published (HCJ1, HCVUS, HCJ4, HCVJH, HCVJ and HCVBK). Homology analysis among the strains revealed that the seven strains were classified into two subtypes; a US subtype (HCJ1 and HCVUS) and a Japanese subtype (HCJ4, HCVJH, HCVJ, HCVBK and HCVNK), since percentage homologies between two subtypes (70.3-77.3%) were significantly lower than those within each subtype (83.9-93.5%). Detailed analysis of the amino acid sequences also indicates that the region at aa246-aa258, tentatively named intersubtype variable region-1, may distinguish the US subtype from the Japanese subtype.
en-copyright=
kn-copyright=
en-aut-name=HadaHajime
en-aut-sei=Hada
en-aut-mei=Hajime
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=KoideNorio
en-aut-sei=Koide
en-aut-mei=Norio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=TakabatakeHiroyuki
en-aut-sei=Takabatake
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=HanafusaTadashi
en-aut-sei=Hanafusa
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=TsujiTakao
en-aut-sei=Tsuji
en-aut-mei=Takao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
affil-num=1
en-affil=
kn-affil=Okayama University
affil-num=2
en-affil=
kn-affil=Okayama University
affil-num=3
en-affil=
kn-affil=Okayama University
affil-num=4
en-affil=
kn-affil=Okayama University
affil-num=5
en-affil=
kn-affil=Okayama University
en-keyword=hapatitis C virus
kn-keyword=hapatitis C virus
en-keyword=envelope
kn-keyword=envelope
en-keyword=DNA sequecing
kn-keyword=DNA sequecing
en-keyword=homology
kn-keyword=homology
en-keyword=intersubtype variable region
kn-keyword=intersubtype variable region
END
start-ver=1.4
cd-journal=joma
no-vol=5
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2005
dt-pub=20050120
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Functional promoter upstream p53 regulatory sequence of IGFBP3 that is silenced by tumor specific methylation
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background: Insulin-like growth factor binding protein (IGFBP)-3 functions as a carrier of insulinlike
growth factors (IGFs) in circulation and a mediator of the growth suppression signal in cells. There are two reported p53 regulatory regions in the IGFBP3 gene; one upstream of the promoter and one intronic. We previously reported a hot spot of promoter hypermethylation of IGFBP-3 in
human hepatocellular carcinomas and derivative cell lines. As the hot spot locates at the putative upstream p53 consensus sequences, these p53 consensus sequences are really functional is a question to be answered.
Methods: In this study, we examined the p53 consensus sequences upstream of the IGFBP-3 promoter for the p53 induced expression of IGFBP-3. Deletion, mutagenesis, and methylation
constructs of IGFBP-3 promoter were assessed in the human hepatoblastoma cell line HepG2 for promoter activity.
Results: Deletions and mutations of these sequences completely abolished the expression of IGFBP-3 in the presence of p53 overexpression. In vitro methylation of these p53 consensus
sequences also suppressed IGFBP-3 expression. In contrast, the expression of IGFBP-3 was not affected in the absence of p53 overexpression. Further, we observed by electrophoresis mobility
shift assay that p53 binding to the promoter region was diminished when methylated.
Conclusion: From these observations, we conclude that four out of eleven p53 consensus sequences upstream of the IGFBP-3 promoter are essential for the p53 induced expression of
IGFBP-3, and hypermethylation of these sequences selectively suppresses p53 induced IGFBP-3 expression in HepG2 cells.
en-copyright=
kn-copyright=
en-aut-name=HanafusaTadashi
en-aut-sei=Hanafusa
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=ShinjiToshiyuki
en-aut-sei=Shinji
en-aut-mei=Toshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=ShirahaHidenori
en-aut-sei=Shiraha
en-aut-mei=Hidenori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=NousoKazuhiro
en-aut-sei=Nouso
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=IwasakiYoshiaki
en-aut-sei=Iwasaki
en-aut-mei=Yoshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=YumotoEichiro
en-aut-sei=Yumoto
en-aut-mei=Eichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=OnoToshiro
en-aut-sei=Ono
en-aut-mei=Toshiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=KoideNorio
en-aut-sei=Koide
en-aut-mei=Norio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
affil-num=1
en-affil=
kn-affil=Okayama University Advanced Science Research Center, Department of Radiation Research, Shikata Laboratory
affil-num=2
en-affil=
kn-affil=Department of Laboratory Medicine, Okayama University Graduate School of Medicine and Dentistry
affil-num=3
en-affil=
kn-affil=First Department of Internal Medicine, Okayama University Graduate School of Medicine and Dentistry
affil-num=4
en-affil=
kn-affil=First Department of Internal Medicine, Okayama University Graduate School of Medicine and Dentistry
affil-num=5
en-affil=
kn-affil=First Department of Internal Medicine, Okayama University Graduate School of Medicine and Dentistry
affil-num=6
en-affil=
kn-affil=First Department of Internal Medicine, Okayama University Graduate School of Medicine and Dentistry
affil-num=7
en-affil=
kn-affil=Okayama University Advanced Science Research Center, Department of Radiation Research, Shikata Laboratory
affil-num=8
en-affil=
kn-affil=Department of Laboratory Medicine, Okayama University Graduate School of Medicine and Dentistry
END
start-ver=1.4
cd-journal=joma
no-vol=30
cd-vols=
no-issue=
article-no=
start-page=33
end-page=37
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=200812
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=岡山大学における核燃料物質の安全管理のための劣化ウランと天然ウランの鑑別について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In Japan, the Law for the Regulation of Nuclear Source Materials, Nuclear Fuel Materials and Reactors (Regulation Law) controls the nuclear fuel materials such as thorium (Th), uranium (U) and plutonium (Pu). Under the Regulation Law, all related materials and reactors are needed to register to the Government. In Okayama University, many nuclear fuel materials, mainly uranium compounds, are registered and stored in 11 departments, separately. Discrimination between depleted uranium and natural uranium is important for the observance of the Regulation Law and the safety management of the nuclear fuel materials in the Okayama University. However, the discrimination of the two kind of uranium has poorly analyzed. In this study, we analyzed several uranium compounds by using γ-ray spectrometry to determine whether the depleted uranium or not.
en-copyright=
kn-copyright=
en-aut-name=NagamatsuTomohiro
en-aut-sei=Nagamatsu
en-aut-mei=Tomohiro
kn-aut-name=永松知洋
kn-aut-sei=永松
kn-aut-mei=知洋
aut-affil-num=1
ORCID=
en-aut-name=HanafusaTadashi
en-aut-sei=Hanafusa
en-aut-mei=Tadashi
kn-aut-name=花房直志
kn-aut-sei=花房
kn-aut-mei=直志
aut-affil-num=2
ORCID=
en-aut-name=KinnoIkuo
en-aut-sei=Kinno
en-aut-mei=Ikuo
kn-aut-name=金野郁雄
kn-aut-sei=金野
kn-aut-mei=郁雄
aut-affil-num=3
ORCID=
en-aut-name=SakodaAkihiro
en-aut-sei=Sakoda
en-aut-mei=Akihiro
kn-aut-name=迫田晃弘
kn-aut-sei=迫田
kn-aut-mei=晃弘
aut-affil-num=4
ORCID=
en-aut-name=HanamotoKatsumi
en-aut-sei=Hanamoto
en-aut-mei=Katsumi
kn-aut-name=花元克巳
kn-aut-sei=花元
kn-aut-mei=克巳
aut-affil-num=5
ORCID=
en-aut-name=YamaokaKiyonori
en-aut-sei=Yamaoka
en-aut-mei=Kiyonori
kn-aut-name=山岡聖典
kn-aut-sei=山岡
kn-aut-mei=聖典
aut-affil-num=6
ORCID=
en-aut-name=OnoToshiro
en-aut-sei=Ono
en-aut-mei=Toshiro
kn-aut-name=小野俊朗
kn-aut-sei=小野
kn-aut-mei=俊朗
aut-affil-num=7
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学大学院保健学研究科
affil-num=2
en-affil=
kn-affil=岡山大学自然生命科学研究支援センター光・放射線情報解析部門鹿田施設
affil-num=3
en-affil=
kn-affil=岡山大学自然生命科学研究支援センター光・放射線情報解析部門鹿田施設
affil-num=4
en-affil=
kn-affil=岡山大学大学院保健学研究科
affil-num=5
en-affil=
kn-affil=岡山大学大学院保健学研究科
affil-num=6
en-affil=
kn-affil=岡山大学大学院保健学研究科
affil-num=7
en-affil=
kn-affil=岡山大学自然生命科学研究支援センター光・放射線情報解析部門鹿田施設
en-keyword=Nuclear Fuel Materials
kn-keyword=Nuclear Fuel Materials
en-keyword=Depleted Uranium
kn-keyword=Depleted Uranium
en-keyword=Natural Uranium
kn-keyword=Natural Uranium
en-keyword=γ-ray Spectrometry
kn-keyword=γ-ray Spectrometry
END
start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=19890328
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=大腸菌フラジｪリン遺伝子の正及び負の発現調節機構
kn-title=Positive and negative regulation in expression of an Escherichia coli K-1'2 flagellin gene
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=花房直志
kn-aut-sei=花房
kn-aut-mei=直志
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学
END