ID | 31626 |
JaLCDOI | |
フルテキストURL | |
著者 |
Matsushita, Akio
Okayama University
Ueoka, Hiroshi
Okayama University
Shibayama, Takuo
Okayama University
Aoe, Keisuke
Okayama University
Kohara, Hiroyuki
Okayama University
Harada, Mine
Okayama University
|
抄録 | We established a drug sensitivity panel consisting of 24 human lung cancer cell lines. Using this panel, we evaluated 26 anti-cancer agents: three alkylators, three platinum compounds, four antimetabolites, one topoisomerase I inhibitor, five topoisomerase II inhibitors, seven antimitotic agents and three tyrosine kinase inhibitors. This panel showed the following: a) Drug sensitivity patterns reflected their clinically-established patterns of action. For example, doxorubicin and etoposide were shown to be active against small cell lung cancer cell lines and mitomycin-C and 5-fluorouracil were active against non-small cell lung cancer cell lines, in agreement with clinical data. b) Correlation analysis of the mean graphs derived from the logarithm of IC50 values of the drugs gave insight into the mechanism of each drug's action. Thus, two drug combinations with reverse or no correlation, such as the combination of cisplatin and vinorelbine, might be good candidates for the ideal two drug combination in the treatment of lung cancer, as is being confirmed in clinical trials. c) Using cluster analysis of the cell lines in the panel with their drug sensitivity patterns, we could classify the cell lines into four groups depending on the drug sensitivity similarity. This classification will be useful to elucidate the cellular mechanism of action and drug resistance. Thus, our drug sensitivity panel will be helpful to explore new drugs or to develop a new combination of anti-cancer agents for the treatment of lung cancer. |
キーワード | drug screening system
MTT assay
lung cancer cell line
drug resistance
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 1999-04
|
巻 | 53巻
|
号 | 2号
|
出版者 | Okayama University Medical School
|
開始ページ | 67
|
終了ページ | 75
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
Web of Science KeyUT |