ID | 31714 |
JaLCDOI | |
フルテキストURL | |
著者 |
Kawada, Kazuhiko
Okayama University
Yonei, Toshiro
National Okayama Medical Center
Ueoka, Hiroshi
Okayama University
Takigawa, Nagio
Okayama University
Harada, Mine
Kyushu University, Fukuoka
|
抄録 | When the development of chemotherapeutic agents reaches the clinical trial stage, it is necessary to perform drug sensitivity tests quickly in order to select the most promising agents for the treatment of cancer. In order to assess the possibility of using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay as a substitute for the human tumor clonogenic assay (HTCA), we evaluated the correlation between the results obtained by these 2 assays in 5 human lung cancer cell lines. The correlation coefficient between the results of the HTCA and the MTT assay was 0.673, indicating a relatively good correlation. The correlation was most prominent in platinum analogues (r = 0.939) and good in anthracyclines/anthracenedione (r = 0.611). However, no significant correlation was observed in vinca alkaloids, etoposide, irinotecan, SN-38 (an active metabolite of irinotecan), and rhizoxin. The results of the MTT assay showed a high degree of correlation with those of the HTCA in predicting the sensitivity of cancer cell lines to platinum analogues, and anthracyclines/anthracenedione. These results suggest that the MTT assay may be more convenient and quickly performed than the HTCA and can replace HTCA in evaluating the effects of anticancer agents, especially the platinum analogues and anthracyclines/anthracenedione. |
キーワード | chemosensitivity test
3-(4
5-dimethylthiazol-2-yl)-2
5-diphenyltertrazolium bromide (MTT) assay
clonogenic assay
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 2002-06
|
巻 | 56巻
|
号 | 3号
|
出版者 | Okayama University Medical School
|
開始ページ | 129
|
終了ページ | 134
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
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PubMed ID | |
Web of Science KeyUT |