start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=5 article-no= start-page=435 end-page=444 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=2023 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Genomic traces of Japanese malting barley breeding in two modern high-quality cultivars, ‘Sukai Golden’ and ‘Sachiho Golden’ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Two modern high-quality Japanese malting barley cultivars, ‘Sukai Golden’ and ‘Sachiho Golden’, were subjected to RNA-sequencing of transcripts extracted from 20-day-old immature seeds. Despite their close relation, 2,419 Sukai Golden-specific and 3,058 Sachiho Golden-specific SNPs were detected in comparison to the genome sequences of two reference cultivars: ‘Morex’ and ‘Haruna Nijo’. Two single nucleotide polymorphism (SNP) clusters respectively showing the incorporation of (1) the barley yellow mosaic virus (BaYMV) resistance gene rym5 from six-row non-malting Chinese landrace Mokusekko 3 on the long arm of 3H, and (2) the anthocyanin-less ant2 gene from a two-row Dutch cultivar on the long arm of 2H were detected specifically in ‘Sukai Golden’. Using 221 recombinant inbred lines of a cross between ‘Ishukushirazu’ and ‘Nishinochikara’, another BaYMV resistance rym3 gene derived from six-row non-malting Japanese cultivar ‘Haganemugi’ was mapped to a 0.4-cM interval on the proximal region of 5H. Haplotype analysis of progenitor accessions of the two modern malting cultivars revealed that rym3 of ‘Haganemugi’ was independently introduced into ‘Sukai Golden’ and ‘Sachiho Golden’. Residual chromosome 5H segments of ‘Haganemugi’ surrounding rym3 were larger in ‘Sukai Golden’. Available results suggest possibilities for malting quality improvement by minimizing residual segments surrounding rym3. en-copyright= kn-copyright= en-aut-name=TaketaShin en-aut-sei=Taketa en-aut-mei=Shin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KimJune-Sik en-aut-sei=Kim en-aut-mei=June-Sik kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakahashiHidekazu en-aut-sei=Takahashi en-aut-mei=Hidekazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YajimaShunsuke en-aut-sei=Yajima en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KoshiishiYuichi en-aut-sei=Koshiishi en-aut-mei=Yuichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SotomeToshinori en-aut-sei=Sotome en-aut-mei=Toshinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KatoTsuneo en-aut-sei=Kato en-aut-mei=Tsuneo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MochidaKeiichi en-aut-sei=Mochida en-aut-mei=Keiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=2 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=3 en-affil=Faculty of Food and Agricultural Sciences, Fukushima University kn-affil= affil-num=4 en-affil=NODAI Genome Research Center, Tokyo University of Agriculture kn-affil= affil-num=5 en-affil=NODAI Genome Research Center, Tokyo University of Agriculture kn-affil= affil-num=6 en-affil=Tochigi Prefectural Agricultural Experiment Station kn-affil= affil-num=7 en-affil=Tochigi Prefectural Agricultural Experiment Station kn-affil= affil-num=8 en-affil=Bioproductivity Informatics Research Team, RIKEN Center for Sustainable Resource Science kn-affil= en-keyword=genetic diversity kn-keyword=genetic diversity en-keyword=Hordeum vulgare kn-keyword=Hordeum vulgare en-keyword=RNA-sequencing kn-keyword=RNA-sequencing en-keyword=seed transcriptome kn-keyword=seed transcriptome en-keyword=single nucleotide polymorphism kn-keyword=single nucleotide polymorphism en-keyword=virus disease resistance genes kn-keyword=virus disease resistance genes END start-ver=1.4 cd-journal=joma no-vol=62 cd-vols= no-issue=3 article-no= start-page=447 end-page=457 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=2021113 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Mutations in a Golden2-Like Gene Cause Reduced Seed Weight in Barley albino lemma 1 Mutants en-subtitle= kn-subtitle= en-abstract= kn-abstract=The albino lemma 1 (alm1) mutants of barley (Hordeum vulgare L.) exhibit obvious chlorophyll-deficient hulls. Hulls are seed-enclosing tissues on the spike, consisting of the lemma and palea. The alm1 phenotype is also expressed in the pericarp, culm nodes and basal leaf sheaths, but leaf blades and awns are normal green. A single recessive nuclear gene controls tissue-specific alm1 phenotypic expression. Positional cloning revealed that the ALM1 gene encodes a Golden 2-like (GLK) transcription factor, HvGLK2, belonging to the GARP subfamily of Myb transcription factors. This finding was validated by genetic evidence indicating that all 10 alm1 mutants studied had a lesion in functionally important regions of HvGLK2, including the three alpha-helix domains, an AREAEAA motif and the GCT box. Transmission electron microscopy revealed that, in lemmas of the alm1.g mutant, the chloroplasts lacked thylakoid membranes, instead of stacked thylakoid grana in wild-type chloroplasts. Compared with wild type, alm1.g plants showed similar levels of leaf photosynthesis but reduced spike photosynthesis by 34%. The alm1.g mutant and the alm1.a mutant showed a reduction in 100-grain weight by 15.8% and 23.1%, respectively. As in other plants, barley has HvGLK2 and a paralog, HvGLK1. In flag leaves and awns, HvGLK2 and HvGLK1 are expressed at moderate levels, but in hulls, HvGLK1 expression was barely detectable compared with HvGLK2. Barley alm1/Hvglk2 mutants exhibit more severe phenotypes than glk2 mutants of other plant species reported to date. The severe alm1 phenotypic expression in multiple tissues indicates that HvGLK2 plays some roles that are nonredundant with HvGLK1. en-copyright= kn-copyright= en-aut-name=TaketaShin en-aut-sei=Taketa en-aut-mei=Shin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HattoriMomoko en-aut-sei=Hattori en-aut-mei=Momoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakamiTsuneaki en-aut-sei=Takami en-aut-mei=Tsuneaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HimiEiko en-aut-sei=Himi en-aut-mei=Eiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SakamotoWataru en-aut-sei=Sakamoto en-aut-mei=Wataru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=2 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=3 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=4 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=5 en-affil=IInstitute of Plant Science and Resources, Okayama University kn-affil= en-keyword=chloroplast kn-keyword=chloroplast en-keyword=GLK2 kn-keyword=GLK2 en-keyword=Hordeum vulgare kn-keyword=Hordeum vulgare en-keyword=photosynthesis kn-keyword=photosynthesis en-keyword=spike kn-keyword=spike en-keyword=thylakoid kn-keyword=thylakoid END start-ver=1.4 cd-journal=joma no-vol=61 cd-vols= no-issue=1 article-no= start-page=35 end-page=42 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=201103 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Expression and functional analysis of the barley Nud gene using transgenic rice en-subtitle= kn-subtitle= en-abstract= kn-abstract=Most cereal crops have hulless grains (naked caryopses) with a free-threshing trait, whereas the majority of barley cultivars show hulled (covered) caryopses. The naked caryopsis in barley is genetically controlled by a single locus, nod. The Nud gene (the covered caryopsis allele) encodes an ethylene response factor (ERF) family transcription factor that regulates a lipid biosynthetic pathway. For functional analysis of the barley Nud gene, we produced transgenic rice expressing Nod in the developing caryopses. All transgenic lines had caryopses that were easily dehulled at maturity, indicating that the naked caryopsis phenotype remained in spite of expression of the Nod transgene. Histochemical and lipid analyses of the transgenic rice caryopses did not show increased lipid accumulation on the surface of developing caryopses, suggesting that the Nud-mediated lipid pathway may not function in rice caryopses. The predicted rice ortholog of Nod, Os06ERF was expressed specifically in the developing caryopses. However, expression of Os06ERF ceased at an earlier developmental stage than that of the native Nod gene in barley caryopses, which was also the case for expression of the Nod transgene. This raises the alternative hypothesis that the timing of Nod expression may be critical for activating the pathway for hull-caryopsis adhesion. en-copyright= kn-copyright= en-aut-name=KakedaKatsuyuki en-aut-sei=Kakeda en-aut-mei=Katsuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IshiharaNorimitsu en-aut-sei=Ishihara en-aut-mei=Norimitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IzumiYohei en-aut-sei=Izumi en-aut-mei=Yohei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SatoKazuhiro en-aut-sei=Sato en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TaketaShin en-aut-sei=Taketa en-aut-mei=Shin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Mie Univ, Grad Sch Bioresources kn-affil= affil-num=2 en-affil=Mie Univ, Grad Sch Bioresources kn-affil= affil-num=3 en-affil=Okayama Univ, Inst Plant Sci & Resources kn-affil= affil-num=4 en-affil=Okayama Univ, Inst Plant Sci & Resources kn-affil= affil-num=5 en-affil=Okayama Univ, Inst Plant Sci & Resources kn-affil= en-keyword=Nud gene kn-keyword=Nud gene en-keyword=transformation kn-keyword=transformation en-keyword=covered/naked caryopsis kn-keyword=covered/naked caryopsis en-keyword=lipid biosynthesis kn-keyword=lipid biosynthesis en-keyword=ERF/AP2 kn-keyword=ERF/AP2 en-keyword=grass kn-keyword=grass END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=1 article-no= start-page=61 end-page=68 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=1997 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Phenol Reaction of Kernels and Chromosome Location of Phenol Reaction Genes in the Genus Triticum kn-title=コムギ属植物における穀粒のフェノール反応の変異およびフェノール反応に関与する遺伝子の座乗染色体 en-subtitle= kn-subtitle= en-abstract=1. コムギ属における穀粒のフェノールによる着色反応を調査した結果、二粒系コムギでは着色する系統としない系統が存在するが、普通系コムギでは3,200以上の品種を供試したにもかかわらず、穀粒がフェノールによって着色しない系統は見いだされなかった。合成コムギ（AABBDD）にはフェノールによって着色しない系統が見出された。2.　異数体分析によって普通系コムギの穀粒のフェノール反応を支配する遺伝子が2Aと2D染色体に座乗することが明らかになり、2B染色体にも穀粒のフェノール反応を支配する遺伝子の存在が認められ、コムギの第2同祖群の全ての染色体にフェノール反応遺伝子が存在することが確かめられた。3.　フェノール反応遺伝子と無葉耳遺伝子が見出されているイネ科植物の染色体の同祖性について考察した。 kn-abstract=A total of 3,606 accessions of the genus Triticum involving diploid, tetraploid, hexaploid, and synthetic wheat and 7 Aegilops materials were tested for the phenol reaction in kernels. All hexaploid wheat showed a positive reaction to phenol, but deffered in staining degree. One of the synthetic wheat lines showed a negative reaction to phenol. Using monosomics, ditelosomics and nulli-tetrasomics in the common wheat cv Chinese Spring (Triticum aestivum L.), and Langdon (Triticum turgidum var. durum) disomic substitutions, genes controlling phenol reaction of kernels were located on chromosomes 2A, 2B and 2D. Synteny of the chromosome region involving the phenol reaction gene in some gramineous plants was discussed. en-copyright= kn-copyright= en-aut-name=ChangCheng Lin en-aut-sei=Chang en-aut-mei=Cheng Lin kn-aut-name=張成林 kn-aut-sei=張 kn-aut-mei=成林 aut-affil-num=1 ORCID= en-aut-name=TakedaShin en-aut-sei=Takeda en-aut-mei=Shin kn-aut-name=武田真 kn-aut-sei=武田 kn-aut-mei=真 aut-affil-num=2 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Phenol reaction kn-keyword=Phenol reaction en-keyword=Triticum kn-keyword=Triticum en-keyword=Chromosome kn-keyword=Chromosome END