ID | 32952 |
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著者 |
Murakami, Jun
Department of Oral and Maxillofacial Radiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
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Asaumi, Jun-ichi
Department of Oral and Maxillofacial Radiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Kawai, Noriko
Department of Oral and Maxillofacial Radiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Tsujigiwa, Hidetsugu
Department of Oral Pathology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
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Yanagi, Yoshinobu
Department of Oral and Maxillofacial Radiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
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Nagatsuka, Hitoshi
Department of Oral Pathology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
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Inoue, Tetsuyoshi
Department of Oral Microbiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
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Kokeguchi, Susumu
Department of Oral Microbiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Kawasaki, Shoji
Department of Radiological Technology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Kuroda, Masahiro
Department of Radiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
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Tanaka, Noriaki
Department of Surgical Oncology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Matsubara, Nagahide
Department of Surgical Oncology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Kishi, Kanji
Department of Oral and Maxillofacial Radiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
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抄録 | We speculated whether or not the expression level of telomerase reverse transcriptase (hTERT) would be modulated by agents targeting epigenetics in oral cancer cell lines. Although hTERT is known to be targeted by epigenetic changes, it remains unclear how chemoagents targeting epigenetics work on hTERT transcription. In the present study, the epigenetic effects of histone deacetylase (HDAC) inhibitor FR901228 on hTERT transcription were analysed by RT-PCR in oral cancer cell lines. The mRNA expression of hTERT was upregulated after exposure to FR901228 in hTERT-negative Hep2 cells, even in the hTERT highly expressed SAS and KB cells. Moreover, co-treatment of protein synthesis inhibitor cycloheximide (CHX) resulted in the induction of hTERT transcription by FR901228. This suggests that the induction of hTERT by FR901228 requires de novo protein synthesis to some extent and is more likely a direct than an indirect effect on epigenetic changes such as histone acetylation / deacetylation. We further examined the effect of FR901228 on c-myc protein, which is one of the main hTERT transcription activators. FR901228 repressed c-myc protein only in the absence of CHX, dependent of the enhancement of de novo protein synthesis. Our results indicate that c-myc protein is repressed indirectly by FR901228 but may not contribute FR901228-induced hTERT transcription. The present study showed that the HDAC inhibitor FR901228 induced the hTERT gene by a complex mechanism that involved other transcription factors except for c-myc, in addition to the inhibition of histone deacetylation.
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キーワード | hTERT
FR901228
oral cancer
HDAC inhibitor
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備考 | Digital Object Identifer:10.1007/10.1007/s00280-004-0976-x
Published with permission from the copyright holder. This is the author's copy, as published in Cancer Chemotherapy and Pharmacology, July 2005, Volume 56, Issue 1, Pages 22-28. Publisher URL:http://dx.doi.org/10.1007/s00280-004-0976-x Direct access to Thomson Web of Science record Copyright © 2005 Springer-Verlag. All rights reserved. |
発行日 | 2005-07
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出版物タイトル |
Cancer Chemotherapy and Pharmacology
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巻 | 56巻
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号 | 1号
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出版者 | Springer
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開始ページ | 22
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終了ページ | 28
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ISSN | 0344-5704
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NCID | AA00598397
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資料タイプ |
学術雑誌論文
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関連URL | author
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言語 |
英語
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著作権者 | Springer-Verlag
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査読 |
有り
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DOI | |
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Web of Science KeyUT | |
Submission Path | dentistry_general/10
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