start-ver=1.4
cd-journal=joma
no-vol=411
cd-vols=
no-issue=3
article-no=
start-page=555
end-page=561
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110805
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Ubiquitin chains in the Dsk2 UBL domain mediate Dsk2 stability and protein degradation in yeast
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Ubiquitin-like (UBL)-ubiquitin-associated (UBA) proteins, including Dsk2 and Rad23, act as delivery factors that target polyubiquitinated substrates to the proteasome. We report here that the Dsk2 UBL domain is ubiquitinated in yeast cells and that Dsk2 ubiquitination of the UBL domain is involved in Dsk2 stability, depending on the Dsk2 UBA domain. Also, Dsk2 lacking ubiquitin chains impaired ubiquitin-dependent protein degradation and decreased the interaction of Dsk2 with polyubiquitinated proteins in cells. Moreover, Dsk2 ubiquitination affected ability to restore the temperature-sensitive growth defect of dsk2 Delta. These results indicate that ubiquitination in the UBL domain of Dsk2 has in vivo functions in the ubiquitin-proteasome pathway in yeast.
en-copyright=
kn-copyright=

en-aut-name=SekiguchiTakeshi
en-aut-sei=Sekiguchi
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SasakiToru
en-aut-sei=Sasaki
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=FunakoshiMinoru
en-aut-sei=Funakoshi
en-aut-mei=Minoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=IshiiTakashi
en-aut-sei=Ishii
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SaitohYoh-hei
en-aut-sei=Saitoh
en-aut-mei=Yoh-hei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KanekoShu-ichi
en-aut-sei=Kaneko
en-aut-mei=Shu-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KobayashiHideki
en-aut-sei=Kobayashi
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University

affil-num=2
en-affil=
kn-affil=Center for Faculty Development, Okayama University

affil-num=3
en-affil=
kn-affil=Center for Faculty Development, Okayama University

affil-num=4
en-affil=
kn-affil=Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University

affil-num=5
en-affil=
kn-affil=Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University

affil-num=6
en-affil=
kn-affil=Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University

affil-num=7
en-affil=
kn-affil=Center for Faculty Development, Okayama University
en-keyword=UBL–UBA protein
kn-keyword=UBL–UBA protein
en-keyword=Dsk2
kn-keyword=Dsk2
en-keyword=UBL domain
kn-keyword=UBL domain
en-keyword=UBA domain
kn-keyword=UBA domain
en-keyword=Ubiquitin
kn-keyword=Ubiquitin
en-keyword=Proteasome
kn-keyword=Proteasome
END