ID | 30724 |
JaLCDOI | |
フルテキストURL | |
著者 |
Fujita, Osamu
Okayama University
Yokoyama, Teruhiko
Okayama University
Ogawa, Norio
Okayama University
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抄録 | We examined the involvement of the signal transducer and activator of transcription 3 (STAT3) in bladder outlet obstruction (BOO)-induced bladder smooth muscle hypertrophy using a rat in vivo and in vitro study. BOO induced increases in bladder weight and bladder smooth muscle thickness 1 week after the operation. By using antibody microarrays, 64 of 389 proteins blotted on the array met our selection criteria of an INR value between > or = 2.0 and < or = 0.5. This result revealed up-regulation of transcription factors, cell cycle regulatory proteins, apoptosis-associated proteins and so on. On the other hand, down-regulation (INR value < or = 0.5) of proteins was not found. In a profiling study, we found an increase in the expression of STAT3. A significant increase in nuclear phosphorylated STAT3 expression was confirmed in bladder smooth muscle tissue by immunohistochemistry and Western blot analysis. Cyclical stretch-relaxation (1 Hz) at 120% elongation significantly increased the expression of STAT3 and of alpha-smooth muscle actin in primary cultured bladder smooth muscle cells. Furthermore, the blockade of STAT3 expression by the transfection of STAT3 small interfering RNA (siRNA) significantly prevented the stretch-induced increase in alpha-smooth muscle actin expression. These results suggest that STAT3 has an important role in the induction of bladder smooth muscle hypertrophy.
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キーワード | benign prostatic hyperplasia
bladder outlet obstruction
bladder smooth muscle
signal transducer and activator of transcription 3 (STAT3)
small interfering RNA (siRNA)
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Amo Type | Original Article
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出版物タイトル |
Acta Medica Okayama
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発行日 | 2006-12
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巻 | 60巻
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号 | 6号
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出版者 | Okayama University Medical School
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開始ページ | 299
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終了ページ | 309
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ISSN | 0386-300X
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NCID | AA00508441
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資料タイプ |
学術雑誌論文
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言語 |
英語
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論文のバージョン | publisher
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査読 |
有り
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PubMed ID | |
Web of Science KeyUT |