start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=973
end-page=979
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Experimental Studies on the Utilization of Iron in Living Body V. A Study on the Iron Metabolism and RES Block
kn-title=生体に於ける鉄の利用に関する実験的研究 第4報 網内系塡塞と鉄代謝に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In the series of this experiments, it was revealed that the reticulo-endothelial system plays a great role in the iron metabolism. The loosely bound colloid iron, whose iron is proved to be easily utilized for hemoglobin-synthesis by intravenous adminstation, is deposited in RES. In this report, the observations on the iron metabolism in the animals having RES blocked with Indian ink and gold colloid sol are presented. The anemic rabbits whose RES is blocked showed a normal decrease in serum iron level but a delayed incorporation of iron into both hem and non-hem fractions of erythorcytes, when Fe(59) is given intravenously as S. I. C.; i. e. hemoglobin synthesis is slightly delayed in the case of gold colloid sol block and markedly in Indian ink block, reaching the normal level after one week in both cases. Histologic observation after the iron adminstration proved the blocked RE-cells can phagocytize the iron colloid. Observation on 13th experimental day showed only the blocking substance but not iron. From these observations the effect of block in RES is mainly in the disturbances of the utilization of the ingested iron but not the phagocytic function itself, though these will be closely related to the nature and the quantity of the materials used for blocking.
en-copyright=
kn-copyright=

en-aut-name=KobayashiJunichi
en-aut-sei=Kobayashi
en-aut-mei=Junichi
kn-aut-name=小林淳一
kn-aut-sei=小林
kn-aut-mei=淳一
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部病理学教室/大学院医学研究科
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=967
end-page=972
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Part 2. On differences of blood stream influencing upon growth of cancer
kn-title=同一胃癌の大彎側と小彎側とにおける発育の差異に関する実験的研究 第2編 発育状況の血流学的差異について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Rabbits were used for this experiment. One of the neighbouring arteries of the stomach was ligated and cut. Thus the distubance of bloodcirculation was made. Then the same amount of suspension of Brown-Pearce tumor was injected into the injured and the other intact part. In the former with local circulating a bigger tumor was built, which grew strongly. From this fact the author believes that, as one one the reason why the stomach cancer is more malignant in the lesser curvaure than in the greater curvature, the stasm of the stomach wall is Playing an important role.
en-copyright=
kn-copyright=

en-aut-name=RyojiSusumu
en-aut-sei=Ryoji
en-aut-mei=Susumu
kn-aut-name=龍治進
kn-aut-sei=龍治
kn-aut-mei=進
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=951
end-page=965
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=On the differences of growth of the same stomach cancer on the greater curvature and lesser curvature. Part 1. On macroscopic and histological differences of growth
kn-title=同一胃癌の大彎側と小彎側とにおける発育の差異に関する実験的研究 第1編 発育状況の肉眼的及び組織学的所見の差異について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The cancer on the side of the greater curvature of the stomach is generally benign. It is considered to owe to that the connective tissue surrounding tumor proliferates more markedly on that side than that on the lesser curvature and suppresses the growth of the cancer by enclosing. Rabbits were used for the following experiments. 1. The same amounts of Brown-Pearce tumor stocks were inoculated at the same in the greater and lesser curvatures of the same rabbit. The growth was always stronger in the lesser curvature. 2. The same amounts of foreign bodies (hard paraffin, silk thread and fluid paraffin) were enbedded or injected at the same time into the greater and lesser curvatures of the same rabbit. The proliferation of surrounding connective tissue was more marked in the greater curvature than in the lesser curvature. 3. The same amounts of Brown-Pearce tumor were inoculated at the same time into the greater and lesser curvatures. The proliferation of connctive tissue surrounding the new tumor was stronger in the greater curvature than in the lesser curvature, but the inflammatory infiltration howed no difference. 4. From the results in 1) and 3) the proliferation of the connective tissue surrounding the tumor is to be considered one of anticarcinomic reactions.
en-copyright=
kn-copyright=

en-aut-name=RyojiSusumu
en-aut-sei=Ryoji
en-aut-mei=Susumu
kn-aut-name=龍治進
kn-aut-sei=龍治
kn-aut-mei=進
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=943
end-page=950
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Metabolism of Glucuronic acid in Vivo Part 2 Supplementary studies on the combination of PAS and studies on the genesis of PAS glycosuria
kn-title=生体内Glucuron酸代謝に関する研究 第二篇 PAS抱合に関する知見補遺並びに糖尿の本態に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=PAS glycosuria was investigated. And the results were as follows. 1. In the patitents with the administion of PAS, the value of glucuronic acid was generally increased in blood and urine, and it's blood value was 8.8mg/dl on the average and it's excretive dosis into urine was 549mg. The Nylander's reaction showed a weak or strong positive in 2 of 17 cases urine. 2. The glucose level in blood and the glucuronic acid level in urine were measured in each fasting and after-meal condition, and the correlation between each of them and Nylander's reaction was investigated. The correlation between the glucose level in blood and the Nylander's reaction was not observed, but the correlation between the glucuronic acid level in urine and the Nylander's reacton was observed. 3. On the esperimental administration of PAS, the excretive dosis of glucuronic acid into urine showed the highest level after 90 or 120 minutes of the administration, in non-hepatic disease or health, and came down to the level, which was shown before the administration, after 4 hours of the administration, but it showed the highest level after 3 or 4 hours of the administration in liver disease. Therefore, it was supposed that PAS was combined with glucuronic acid and excreted into urine and this combination had a connection with liver function. 4. Since the results that glucuronic acid was separated from the PAS-glycosuria which was experimentally provoked on rabbit and was qualitatively tested, it was supposed that there was the compound of glucuronic acid and PAS and the combination was an esterfrorm. On the examination of the separated solution by the paper chromatography, the existence of glucose was denied and the separation between PAS and glucuronic acid could be obtained after the hydrolysis. The absorption curve of the separated solution displayed the absorption maxima at 269 and 390mμ and, after it,s hydrolysis, it showed the absorption curve, with the absorption maximum at 270mμ, which agreed with the absorption curve of PAS. The absorption curve of O-aminophenol glucuronide, as a control, displayed the absorption maxima at 270 and 285mμ and, after it's hydrolysis, it showed the absorption curve, with the absorption maximum at 285mμ, which agreed with the absorption curve of O-aminophenol. 5. Therefore, it was concluded that the genesis of PAS-glycosuria was a combined glucuronic acid with PAS.
en-copyright=
kn-copyright=

en-aut-name=ShokakuMasatoshi
en-aut-sei=Shokaku
en-aut-mei=Masatoshi
kn-aut-name=正覚正利
kn-aut-sei=正覚
kn-aut-mei=正利
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=933
end-page=942
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Metabolism of Glucuronic Acid in vivo Part 1 Studies on the determinative methods of glucuronic acid value and the clinical significance
kn-title=生体内Glucuron酸代謝に関する研究 第一篇 定量法の検討並びに臨床実験的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=It was well known that glucuronic acid played a important role as a combining antidote in vivo, but few determinative methods of glucuronic acid value are useful for a clinical aplication. Therefore, the determinative methods of glucuronic acid were first investigated and then glucuronic acid value in blood and urine, in health and disease, were observed. And the results were as follows. 1. The Fishman's determinative method of glucuronic acid value was confirmed to be useful for a clinical application, since it was compared with the original method for the determination of glucuronic acid value on the minuteness, singularity and recovered property by the use of naphthoresorcinolcarbon acid barium. 2. The value of glucuronic acid in blood of healthy adults was 7.1 mg/dl in male and 6.8 mg/dl in female, and it's total excretive dosis in urine was 363.7 mg in male ang 327 mg in female. The density of glucuronic acid in blood showed no change for 24 hours, but it's excretion in urine was so high in the morning that the total execretive dosis of glucuronic acid into urine should be measured with 24 hours' urine. 3. As the singularity was not observed on the excretive dosis of glucuronic acid by the intravenous administration of glucose, it was thought that glucose did not directly participate in the combination of glucuronic acid. 4. Glucuronic acid was, in some degrees, accumulated in blood by the administration of glucuronic acid, and it could be partly utilized for the combination. 5. In liver diseases, the total excretive dosis of glucuronic acid into urine showed markedly decreased and the value of glucuronic acid in blood also showed decreased with the degree of liver damage, but at the early stage of acute hepatitis, some cases showed the high value of glucuronic acid in blood, in opposition to the decreasion of it's excretion into urine. 6. In the cases with longstanding fever, like the case of subacute bacterial endocarditis, the excretive increasion of glucuronic acid into urine was observed. 7. In the cases with the administration of PAS, the total excretive dosis of glucuronic acid into urine showed the increasion, and it was thought that PAS was participated in the combination of glucuronic acid. 8. In half of the cases with habitual constipation the excretion of glucuronic acid into urine showed the increasion, but it became showing the normal value on the measurement of glucuronic acid after thebowel movement in those cases showed the high excretion of glucuronic acid into urine. 9. In other diseases, Basedowe disease, sypinal sphilis, thrombocytopenic purpura, stomach ulcer, duodenal ulcer, myelitis and cholecystopathy etc., no significant tendency was observed on the excretion of glucuronic acid.
en-copyright=
kn-copyright=

en-aut-name=ShokakuMasatoshi
en-aut-sei=Shokaku
en-aut-mei=Masatoshi
kn-aut-name=正覚正利
kn-aut-sei=正覚
kn-aut-mei=正利
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=919
end-page=931
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Morphological Studies on the Growth Mechanism of DAB-Hepatoma
kn-title=DAB肝癌の増殖機構に関する形態学的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With the purpose to elucidate the growth mechanism of cancer cells the structural arrangement of cancer cells and the newly developed vessels in cancer tissues have been studied morphologically on the reconstruction models obtained from the serial sections of the hepatoma induced experimentally in albino rats by feeding DAB, either in the original form or after irrigating it with India ink. The distribution of blood vessels in the hepatoma tissue is found to be poor and yet extremely irregular in pattern. India ink introduced through the artery or portal vein proved to be hard to reach the vessels of tumor tissues. This can be recognized both macroscopically and histologically as well. In pursuit of the mutual relationship between the development of cancer lobules and the blood vessels it has been revealed that in furthest the cancer cells are proliferating at the distance of 80 to 200μ away from the nearest vessel wall and as for the cell layer there are about 12 to 22 cancer cells, 15 cells on average, existing in between the furthest cell group and the vessel wall. However, in the area futher away from this distance the necrosis of the cells are found suggesting the cancer cells can not live there. The necrosis of the cell will be caused by the oxygen deficiency as it is supposed that any other anabolic or catabolic material is easier to be transferred by their higher diffusion constants than that of oxygen. From these morphologic observations and in view of theoretical consideraion on the oxygen tension in the cancer tissues it is deduced that the cancer cells require only an extremely small amount of oxygen but cannot live in complete absence of oxygen. Mitosis is seen frequently in the region surrounding the vessels and become rare in the parts apart from the vessels. On the basis of these observations the author discussed about the developing modes of cancer cells in the relation with the vessels, comparing his own experimental results with those presented by other investigators on the morphologic structure of blood vessels in various tumor tissues.
en-copyright=
kn-copyright=

en-aut-name=AkagiSeiji
en-aut-sei=Akagi
en-aut-mei=Seiji
kn-aut-name=赤木制二
kn-aut-sei=赤木
kn-aut-mei=制二
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部病理学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=901
end-page=918
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Clinical and Pathological Studies ou the Small Tubercular Lesions which Produce Atypical Shadows on the Chest X-ray. Ⅱ: Pathological Study
kn-title=胸部x線的不定型陰影を呈する結核性小病変の臨床並びに病理学的研究 第2編 病理学的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In 250 cases of lung resection (200 cases with cavity and 50 cases with tuberculoma), radiographic examination, sputa tests and other clinical findings were compared with pathological and bacteriological findidgs of the excised samples. 1. Four cases out of five with the string-form shadows on x-ray picture had cicatricated lesions, and the one had pre-cicatricated lesions. Both of these are stabilized tubercular foci. Most of the cases with the asterial, club-form or lump-form shadows had remaining cavities or covered caseous lesions. Motley shadows were found in one case with contracted tuberculoma, which was considered to be in the healing process. Especially, pre-ciatricated lesions which developed after tuberculoma may be considered to be stabilized, healing foci. 2. Out of 30 cases, which had shown the atypical shadows developed from the cavity on the x-ray picture, 7 cases (or 23.3%) were in the healing process, 4 cases being cicatricatedand 3 cases being in the pre-cicatricated stage. The rest (23 cases or 76.7%) kept bacilli-positive caseous substances in the lung and had the possibility of relapse. 3. Out of 4 cases, which had shown shown the atypical shadows developed from the tuberculoma on the x-ray, 3 cases (or 75%) were almost healt with pre-cicatricated lesions without bacilli.
en-copyright=
kn-copyright=

en-aut-name=MorishigeTeruo
en-aut-sei=Morishige
en-aut-mei=Teruo
kn-aut-name=森重照夫
kn-aut-sei=森重
kn-aut-mei=照夫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=国立療養所山陽荘
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=885
end-page=900
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Clincal and Pathological Studies on the Small Tubercular Lesions Which Produce Atypical Shadows on The Chest X-ray. Ⅰ: Clinical Study
kn-title=胸部x線的不定型陰影を呈する結核性小病変の臨床並びに病理学的研究 第1編 臨床的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Radiographic changes in 206 cases with small cavernous lesions of pulmonary tuberculosis and in 50 cases with tuberculoma which had received chemotherapy for more than 4 months were investigated, and the following conclusions were drawn. 1. Atypical shadows due to these changes were able to be classified into 5 types: string-form, asterial, club-form, motley, and lump-from. 2. The atypical shadows were observed in 17.8% of the casel with cavernous tubercular lesions and in 8% of tuberculoma cases. 3. The cavernous lesions, which were fresh with the surrounding infiltration and had a diameter less than 2cm., tended to produce the atypical shadows. The longer the duration of chemotherapy, the higher the tendency to develop the atypical shadows. It occurred most frequently in 6-8 months after the onset of treatment. Systematic treatments were proved to be superior to non-sys-tematic treatments. No difference was observed between the effect of INH and othe agents in this regard. 4. In case of tuberculoma, there was no apparent relations between the occurrence of atypical shadows and the size of lesion or the duration of treatment. The difference between the effect of INH and other agents was not observed in these cases either. 5. Tubercular bacillus was negative in the sputa of 90% of cavernous cases and 100% of tuberculoma cases which showed the atypical shadows. Most of cases with positive sputa were found to have the lump-type shadows on chest x-ray. Most of cavernous cases and some tuberculoma cases which did not show the atypical shadows had positive sputa. A large number of these positive cases showed resistance more than 10γ to any of the anti-tubercular chemotherapeutics. 6. The cavities became open again in a short time in some cases which once had had negative sputa and had shown the string-form shadows.
en-copyright=
kn-copyright=

en-aut-name=MorishigeTeruo
en-aut-sei=Morishige
en-aut-mei=Teruo
kn-aut-name=森重照夫
kn-aut-sei=森重
kn-aut-mei=照夫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=国立療養所山陽荘
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=879
end-page=883
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Reticulocyte Ripening Subsuances Part 3. The cellulor Origin and the characterisbics of the Ripening Substance
kn-title=網赤血球成熟物質に関する研究 第三編 網赤血球成熟物質の起原とその性格
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=From the results presented in Part 2 it is supposed that the reticulocyte ripening substances in the bovine liver found by the author may be protein belonging to a globulin fraction or a substance attached to some particles of the cytoplasm, which can not be precipitated by the general centrifugation. The supernatant obtained from the saline extract by centrifuging at a low speed was again put to ultra-centrigu-gation at 40,000 r. p. m., thus splitting fraction into two, soluble fractions and microsome or mitochondrial fractions. Ripening test on reticulocytes proved that the effective substance had been transfered to the soluble fraction. The globulin fraction obtained from the saline exfract by the half saturation with ammonium sulfate also proved to be effective. Electro-paper chromatography of the protein obtained from the saline extract revealed that it contained a fraction of γ-globulin which was found to be lacking in the water extract. Thus the ripening substance of reticulocyte has been prove to be a protein belonging to the γ-globulin fraction originated from the hyaloplasm.
en-copyright=
kn-copyright=

en-aut-name=SanadaHiroshi
en-aut-sei=Sanada
en-aut-mei=Hiroshi
kn-aut-name=真田博史
kn-aut-sei=真田
kn-aut-mei=博史
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部病理学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=873
end-page=878
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Reticulocyte Ripening Substances Part 2. The Extraction of Reticulocyte Ripening Substances from the Bovine Liver
kn-title=網赤血球成熟物質に関する研究 第二編 牛肝内網赤血球成熟物質の抽出
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The attempt has been made to extract the reticulocyte ripening substances from bovine liver with distilled water, a 2% saline solution, ethanol and ether. As the results it has been ascertained that the ripening substances can be extracted by the saline solution but not by distilled water, ethanol, ether.
en-copyright=
kn-copyright=

en-aut-name=SanadaHiroshi
en-aut-sei=Sanada
en-aut-mei=Hiroshi
kn-aut-name=真田博史
kn-aut-sei=真田
kn-aut-mei=博史
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部病理学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=867
end-page=872
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Reticulocyte Ripening Substances Part 1. The Action of Bovine Liver Extract on the Maturation of Reticulocytes in Rabbits
kn-title=網赤血球成熟物質に関する研究 第一編 家兎網赤血球の成熟に及ぼす牛肝エキスの作用について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=According to the method deviced by Seno and orthers the mode of maturation of reticulocytes of anemic rabbits have been observed in vitro with or without addition of the emulsion of bovine liver. The observations prove that the supernatant of the liver emulsion has the ability to accelerate the maturation of the reticulocytes showing complete maturation by about two hours in the oxygenated blood where the supernatant is added, while it takes about 9 hours under the same condition without addition of the suppernatant. Intravenous introduction of a large dose of the supernatant into the anemic rabbits results in a temporary and rapid derease of reticulocytes in circulating blood. These observations indicate that the ripening substances like that in guinea pig liver found by Seno and others likewise exist in the bovine liver.
en-copyright=
kn-copyright=

en-aut-name=SanadaHiroshi
en-aut-sei=Sanada
en-aut-mei=Hiroshi
kn-aut-name=真田博史
kn-aut-sei=真田
kn-aut-mei=博史
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部病理学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=849
end-page=865
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Extraction Substances of Dysentery Bacillus by Means of Middlebrook's Hemolytic Reaction 1. Comparative Study on Chemical Natures of Extraction Substances 2. Comparative Study on Hemolytic Mature of Extraction Substances 3. Hemolytic Nature of Diluted Antigens
kn-title=Middlebrookの溶血反応による赤痢菌体抽出物質の研究 1) 菌体抽出物質の化学的性状の比較 2) 菌体抽出物質の溶血性状の比較 3) 抗原溶液の濃度別に見た溶血性状
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Of Various extraction substances of Shigella sonnei, the author studied the chemical natures, the hemolytic specificity and the hemolysis by various diluted fractions, and, in addition to these, comparatively studied the natures of various extraction substances of S and R types. The results are briefly summarized as follows: 1) As for the chemical natures of various fractions, particularly of nucleic acids, the nucleic acid extracted with phenol was fairly purer than nucleic acid II extracted with saline and weak alkaline solutions and deproteinized by chloroform-gel and trypsin digestion method. 2) In the hemolysis, hemolytic titer of nucleic acid group substances rised with the progress of purification procedures; this fraction was considered to be a significant one as the antigen. 3) In the hemolysis by various concentration of the antigen, no difference was observed between those of chick and rabbit red cells by the 1mg/ml purified nucleic acid II, but a clear differnce existed between the two by 10γ/ml of it. Chick red cells were better sensitized by nucleic acid group substances than Boivin's substance, and rabbit red cells showed a reversse relationship. 4. As for the S and R type difference, polysaccharide substances showed a specific difference; the CF and Boivin's substances of S type showed a stronger red cell sensitization those of R type.
en-copyright=
kn-copyright=

en-aut-name=NakayamaGoro
en-aut-sei=Nakayama
en-aut-mei=Goro
kn-aut-name=中山五郎
kn-aut-sei=中山
kn-aut-mei=五郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=841
end-page=848
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Studies on Fat Embolism in the Organs at the Time of Wound Report 2 The Study on Fat Embolism in the Organs of the Animals at the Time of Wound
kn-title=受傷時における臓器組織中遊離脂肪の意義 第Ⅱ編 動物実験による受傷時におけの臓器組織中遊離脂肪の意義
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author made an experimental study with guinea-pigs and detected fat embolism in the lung in the same way as the report 1. The author obtained the following results. 1) It is confirmed that the occurrence of the fat embolism in the lung were the strongest in the case of injury on bones. There was proved the fat embolism in the lung also in the case of vertebral fracture. In the case of injury on soft part tissues by incised wounds, punctured wounds, cut wounds and contused wounds, there was proved an equal slight fat embolism in the lung. 2) There was slightly high degree of fat embolism in the lung in the case of injury on head rather than in the case of injury on breast, abdomen, and hands and feet. 3) There was confirmed the high degree of fat embolism in the lung in the case when the bodies have been left for about several minutes or several hours before death after wounded than those of sudden death, and showed a decline of fat embolism in the case when the bodies have been left for about 10 hours after wounded. 4) There was proved a remarkable fat embolism in the lung in the all cases of being run over and killed, and also proved a slight fat embolism in the case of death from burns. It is thought that these detections have a deep significance as a sign of vital reaction. 5) There was nothing in the cases of death from hanging, strangulation, strangulation by the hand and death of intoxication (carbon monoxide, potassium cyanide, arsenious acid and luminal). 6) The fat embolism that have been appeared in the lung showed no change by post-mortem operations such as being run over and killed, hanging and strangulation. 7) There was not proved the fat embolism in the lung by the decomposition of dead bodies, and the substance that would be dyed red by Sudan Ⅲ.
en-copyright=
kn-copyright=

en-aut-name=KusakaHironao
en-aut-sei=Kusaka
en-aut-mei=Hironao
kn-aut-name=草加宏直
kn-aut-sei=草加
kn-aut-mei=宏直
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部法医学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=833
end-page=840
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Experimental Studies on the Changes by Death of the Vegetative Plankton (Diatoms) in the Organs of Drowned Bodies Report 2. The Experimental Studeis on the Changes by Death of the Vegetative Plankton (Diatoms) in the Organs of Drowned Bodies in Summar
kn-title=溺死体臓器における植物性Plankton珪藻の死後における浩長に関する実験的研究 第Ⅱ編 夏季の溺死体臓器における植物性Plankton珪藻の死後における消長について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author, continuing the experiment of spring and autumn, had an experiment on the changes by death, of the vegetative Plankton (diatoms) in the organs of drowned bodies in summar, and has obtained the following results: 1. There were 11 diatoms from the water of the river as well as the experiments of spring and autumn. 2. There were 8 among 11 kinds of diatoms which were found in the lung for three days after death, the utmost limited time for use. 3. There were 3 among 11 kinds of diatoms which were found in the heart, liver and kidney for three days after death, the utmost limited time for use, they are Cymbella parva, Melosira islandica and Cyclotella comta which have been considered to have strength for resisting to decomposition. 4. Among these 11, 5 diatoms such as Cymbella parva, Navicula radiosa, Melosira islandica, Cyclotella comta, and Rhopalodia giba were found in the femur even two months after death. 5. From the experiment above, the author has come to the conclusions. In summar when the progress of decomposition is quick, the organs of drowned bodies melt and it is for use, the diatoms stay in the femur for the long term; this can be said to be the basis in the explanation whether drowning is the cause of death.
en-copyright=
kn-copyright=

en-aut-name=ShiragamiKiyotoshi
en-aut-sei=Shiragami
en-aut-mei=Kiyotoshi
kn-aut-name=白神清敏
kn-aut-sei=白神
kn-aut-mei=清敏
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部法医学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=825
end-page=831
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Enzymatic Properties of Shigella Dysenteriae Part 2. Catalase and Peroxidase Activities
kn-title=Sh. dysenteriaeの酵素的性状について 第2篇 catalase, peroxidase作用
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By studying the influences of H(2)O(2) on the catalase and peroxidase actions and metabolisms of cells belonging to Sh. dysenteri 1-7, the author obtained the following results: 1. No evidence of H(2)O(2) accumulation either by cells with catalase or those without catalase can at all be seen during the growth or glucose oxidation. 2. In the relationshp betwen the peroxidase activity and H(2)O(2) concentration, the cells possessing catalase differs from without catalase. 3. The addition of glucose even in the case of cells lacking catase will enable to dispose of H(2)O(2). 4. In the case of the cells possesing no catalase, it seems that H(2)O(2) is not a all produced in the course of the metabolism, or some H(2)O(2) is produced momentarily but such H(2)O(2) is immediately disposed of by pyruvate or other substances produced during the metabolism.
en-copyright=
kn-copyright=

en-aut-name=NanbaToshio
en-aut-sei=Nanba
en-aut-mei=Toshio
kn-aut-name=難波敏夫
kn-aut-sei=難波
kn-aut-mei=敏夫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=815
end-page=824
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Enzymatic Properties of Shigella dysenteri ea Part 1. The Nutrition Requirement for Growth and the Substrate Oxidation of Resting Cells
kn-title=Sh. dysenteriaeの酵素的性状について 第1篇 発育に於ける栄養要求, 及び静止菌の基質酸化
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Using the standard strains belonging to Sh. dysenteri 1. (A1)-7 (A7) as the test bacteria, the author studied the nutrition requirement of the growtn and enzymatic properties of these cells; and obtained the following results: 1. In comparing the catalase activity of each cell A(6) and A(7) show a marked activity, and A(2) slightly, while A(1), A(3), A(4), and A(5) show no activity at all. 2. When the nutrition requirements for the growth are compared, A(7) is found to be able to grow in the most simple medium of all, followed by A(6) and A(2). A(1), A(3), A(4) and A(5) possessing no catalase show a tendency to require a more stringent nutrition than those possessing catalase. 3. Looking over the effects of several inhibitory agents against the glucose oxidation of these cells, no clear-cut difference can be observed between the cells with catalaes and without catalase.
en-copyright=
kn-copyright=

en-aut-name=NanbaToshio
en-aut-sei=Nanba
en-aut-mei=Toshio
kn-aut-name=難波敏夫
kn-aut-sei=難波
kn-aut-mei=敏夫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=805
end-page=813
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Utilization of Aureomycin as the Inhibitor in the Study of Bacterial Glucose oxidation. Part 2. Mechanism of Aureomycin inhibition
kn-title=細菌のグルコース酸化に於ける阻害剤としてのオーレオマイシンの利用 第2篇 オーレオマイシン阻害の機序
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Using the standard straine of Sh. flexneri 2a and St. aureus, the mechanism of AM inhibition on the c-source oxidation of bacteria are studied and the following results ore obtained. 1. AM inhibition of glucose, lactate or pyruvate oxidation can be restored by addition of Fe(++) or Fe(+++), especialy in th case where Fe(++) or Fe(+++) added to cells before addition of AM. 2. AM does not inhibit so markedly the oxygen uptake of the cells grown on the medium added KCN or NaN(3) and adapted to these inhibitors. From these results, it seems that AM inhibits the enzymatic retions by catching Fe(++) the other metal ions necessary to the enzyme actviities. 3. AM inhibition can not be restored by thionine, and AM does not inhibit catalase and peroxidase reaction.
en-copyright=
kn-copyright=

en-aut-name=IzumiharaMitsue
en-aut-sei=Izumihara
en-aut-mei=Mitsue
kn-aut-name=泉原充衛
kn-aut-sei=泉原
kn-aut-mei=充衛
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=799
end-page=804
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Utilization of Aureomycin as the Inhibitor in the Study of Bacterial Glucose oxidation. Part 1 Action of Aureomycin on the oxidation of Glucose and Gluconate
kn-title=細菌のグルコース酸化に於ける阻害剤としてのオーレオマイシンの利用 第1篇 グルコース,グルコン酸の酸化に於けるオーレオマシンの作用
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With the use of Sh. flexneri 2a and St. aureus as the test bacteria the auther studied the action of AM (aureomycin) as an inhibitor on the bacterial glucose and gluconate oxidation. The results are as follows: 1. AM at the concentration about 10(-4)M inhibits the oxygen consumption of bacteria in the oxidation of glucose, gluconate, ribose, lactate, pyruvate or succinate to the extent of 50 % . Moreovere in the case of ribose or pyruvate the inhibition is most markedly. 2. AM accerelates the accumulation from glucose. It seems that AM inhibits pyruvate oxidation, and the transfer of H from substrate to O(2). 3. AM accerelates the C(5)-sugar accumulation from gluconate. One of points of action fo AM seems to be C(5)-sugar oxidation.
en-copyright=
kn-copyright=

en-aut-name=IzumiharaMitsue
en-aut-sei=Izumihara
en-aut-mei=Mitsue
kn-aut-name=泉原充衛
kn-aut-sei=泉原
kn-aut-mei=充衛
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=791
end-page=798
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Brain Hexokinase Part Ⅲ. On hexokinase in the cerebral cortex of epileptics
kn-title=脳hexokinaseに関する研究 第3編 真正癲癇患者大脳皮質のhexokinaseについて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The hexokinase activity in the human cerebral cortex of the epileptics as well as the non-epileptics was measured, and the influences of glutamic acid, glutamine, aspartic acid, asparagine, γ-aminobutyric acid and α-ketoglutaric acid upon the activity were evaluated and compared. The results were as follows. 1) The hexokinase activity in the cerebral cortex of the genuine epileptics is generally decreased, compared with that of the non-epileptics. 2) This decrease in the epiliptics is recovered and increased by asparagine, glutamic acid, γ-aminobutyric acid, aspartic acid and glutamine.　3) α-Ketoglutaric acid markedly inhibits the hexokinase activity even in the epileptics.
en-copyright=
kn-copyright=

en-aut-name=YamadaTakahiko
en-aut-sei=Yamada
en-aut-mei=Takahiko
kn-aut-name=山田孝彦
kn-aut-sei=山田
kn-aut-mei=孝彦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1（陣内）外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=785
end-page=790
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Brain Hexokinase Part Ⅱ. On hexokinase in the cerebral cortex of rabbits with latent cerebral local anaphylaxis
kn-title=腦hexokinaseに関する研究 第2編 潜在性脳局所アナフィラキシー家兎大脳皮質のhexokinaseについて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Latent cerebral local anaphylaxis (LCLA) has been experimentally probed to be an epileptic disposition and a state of arrangement of convulsion by many reports. The author measured the hexokinase activity in the cerebral cortex of rabbits with latent cerebral local anaphylaxis which was experimentally influenced by the use of glutamic acid, glutamine, aspartic acid, asparagine, γ-aminobutyric acid and α-ketoglutaric acid and the following results were obtained. 1) The hexokinase activity in the cerebral cortex of rabbits with LCLA is decreased, compared with that of normal rabbits. 2) Asparagine markedly accelerates the activity, while γ-aminobutyric acid does slightly. 3) Glutamic acid, glutamine or aspartic acid has almost no influence on the activity. 4) α-Ketoglutaric acid shows a marked inhibition to the activity as seen in normal rabbits.
en-copyright=
kn-copyright=

en-aut-name=YamadaTakahiko
en-aut-sei=Yamada
en-aut-mei=Takahiko
kn-aut-name=山田孝彦
kn-aut-sei=山田
kn-aut-mei=孝彦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1（陣内）外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=779
end-page=784
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Brain Hexokinase. Part Ⅰ. On hexokinase in the cerebral cortex of rabbits
kn-title=腦hexokinaseに関する研究 第1編 家兎大脳皮質のhexokinaseについて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The hexokinase activity in the cerebral cortex of normal rabbits was measured by Longs method and the influences of fluoride, Ca ion, glutamic acid, glutamine, aspartic acid, asparagine, γ-aminobutyric acid and α-ketoglutaric acid upon the hexokinase activity were also evaluated. The results were as follows: 1) The fluoride shows the maximum inhibition to the hexokinase activity at 0.5M of its concentration, and the inhibition rather decreases at higher concentration. 2) Ca ion shows an increasing inhibition as its concentration increases. 3) When asparagine or glutamic acid is added, the activity is markedly accelerated. 4) γ-Aminobutyric acid accelerates slightly the activity of the cerebral hexokinase and markedly that of the heart muscle hexokinase. 5) α-Ketoglutaric acid markedly inhibits the activity.
en-copyright=
kn-copyright=

en-aut-name=YamadaTakahiko
en-aut-sei=Yamada
en-aut-mei=Takahiko
kn-aut-name=山田孝彦
kn-aut-sei=山田
kn-aut-mei=孝彦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1（陣内）外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=771
end-page=777
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Electronmicroscopic Studies on Supravitally-Stained Granules Part 3. An Electronmicroscopic Studies on Substantia Granulo-Filamentosa (SGF) of Reticulocytes produced by Nile Blue Supravital Staining
kn-title=超生体染色顆粒の電子顕微鏡的研究 第3編 Nile blue超生体染色によつて生ずる網赤血球Substantia Granulo-Filamentosa〔SGF〕に関する電子顕微鏡的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In this paper, the author reports the mechanism of the formation of the Substantia Granulo-Filamentosa (SGF) in reticulocytes produced by supravital staining. The reticulocytes have been obtained from the circulating blood of the blood depleted anaemic rabbits in the stage of the reticulocyte crisis. A droplet of blood taken was stained supravitally mixing with Nile blue solution (Hanks' solution containing 0.1% Nile blue.) and fixed with the method of author's own device as mentioned in Part 1, by which the dye can be fixed and appears electron dense under electron microscope. By preparing ultrathin sections, these reticulocytes have been observed under electron microscope comparing with the section of the reticulocytes fixed with 1.0% osmic acid. And it has been verified that the SGF of reitculocytes appear as the electron dense sbustances irregular in shape, mainly tangling around the mitochondria. The mitochondria are not stained, showing no increased electron density, though they are swollen and have deformed cristae. It has been verified that Substantia Granulo-Filamentosa (SGF) of reticulocytes are artificial product produced as the result of agglomeration of endoplasmic reticulum due to the presence of Nile blue, and that mitochondria are not involved in the stained picture of SGF.
en-copyright=
kn-copyright=

en-aut-name=NakamotoTakashi
en-aut-sei=Nakamoto
en-aut-mei=Takashi
kn-aut-name=中本孝
kn-aut-sei=中本
kn-aut-mei=孝
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部病理学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=765
end-page=770
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Electronmicroscopic Studies on Supravitally-Stained Granules Part 2. Electronmicroscopic Studies on Supravital Staining of Golgi Apparatus by Neutral Red
kn-title=超生休染色顆粒の電子顕微鏡的研究 第2編 Neutral redによるGolgi装置の超生体染色に関する電子顕微鏡的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=After fixing the bone marrow cells supravitally stained with neutral red by the method, the auther's own device as mentioned in Part 1, the author succeeded in detecting supravitally stained neutral red granules under the electronmicroscope. As the result, it has been clarified that neutral red does not stain Golgi apparatus specifically as generally believed nor does it stain specifically any other organellae in the cell. Namely, the dye is found within cytoplasma only in the form of dye droplets independent of organellae. It is assumed that the tendency of this dye-droplets gathering especially in the region of Golgi apparatus is due to the mechanism which attracts all the foreign substances invaiding the cytoplasma in that region or to a purely physicochemical phenomenon, the gathering at the boundering of two different colloid phases.
en-copyright=
kn-copyright=

en-aut-name=NakamotoTakashi
en-aut-sei=Nakamoto
en-aut-mei=Takashi
kn-aut-name=中本孝
kn-aut-sei=中本
kn-aut-mei=孝
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部病理学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=757
end-page=764
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Electronmicroscopic Studies on Supravitally-Stained Granules Part 1. A New Method of Fixation for Dyes of Supravitally-Stained for Electronmicroscope and Observation of the Janus Green B Granules by this Methods
kn-title=超生体染色顆粒の電子顕微鏡的研究 第1編 電子顕微鏡的研究のための超生体染色々素固定方法並びにこの方法によるJanus green B顆位の観察
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The auther successfully fixed stained granules of the supravitally stained bone marrow cells by the use of mercuric potassium iodide solution for quantative determination of NH(3). Insoluble complex of the dye can be produced by the reaction between dimethylamino rdaicals of the dye and mercuric potassium iodide. The fine structure of the protoplasma can be retained by using mercuric potassium iodide solution added an equal volume of 2% osmic acid solution (pH 7.4) for the fixation. Electronmicroscopic observation on the bone marrow cells stained supravitally with Janus green B and fixed by the solution just mentioned revealed that Janus green B granules appear as electron dense mass or rings having thick walls and it has been proved these granules are nothing but the mitochondria themselves, from the morphologic pictures of those less electron dense granules. The electron density of the granules should be given by mercury. As the reaction of the dye with mercuric potassium iodide should occur in both colored and non-colored leuco-Janus green as well, because the reaction has no relation with reduction or oxidation of the dye, then, the results, show that Janus green B stains mitochondria selectively, but on other elements of cytoplasma. Osmic acid is proven to have an ability to oxidize the leuco-Janus green B to a colored one in vitro, and the light microscopic observations of the cells treated with the fixatives just mentioned showed only the green mitochondria without any coloration of other cytoplasmic area. This again shows the Janus green enters into mitochondrira selectively. These observations refute the theory propored by Lazarow and Cooperstein in which they claim that the Janus green entors into the cytoplasma diffusely, but mitochondria only can be recognized as the stained granules by the oxydation of Janus green B. But, there are some of mitochondria remain without staining. Any other cytoplasmic elements are not stained by Janus green B as far as the cells the auther observed. As an exceptional case, in reticulocyte, Janus green stains endoplasmic reticulum but not the mitochondria, giving the picture of Substantia Granulo-Fiamentosa (SGF), themselves. This may mean the changes in chemical components of the organellae in reticulocytes as they are differentiated to synthesize haemoglobin.
en-copyright=
kn-copyright=

en-aut-name=NakamotoTakashi
en-aut-sei=Nakamoto
en-aut-mei=Takashi
kn-aut-name=中本孝
kn-aut-sei=中本
kn-aut-mei=孝
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部病理学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=737
end-page=756
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Study of the Effect on the Enzymic Action in Organs by Administrating Nutriment at the Time of Experimental Starvation
kn-title=実験的飢餓時における栄養素投与による臓器酵素作用の影響について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author has conducted the experiment classifying some rabbits into four groups: 1) the group of complete starvation, 2) the group of incomplete starvation being administrated mononutriment, 3) the group of incomplete starvation being administerated two kinds of nutriment, 4) the group of prolonged incomplete starvation being administrated mononutriment at the end of complete starvation. The author, then, examined whether a few hydrolytic enzymic actions in organs at the time of starvation would be effected by administrating nutriment, and arrived at the following results: 1) It is methionin that prolongs the living days most under starvation. 2) The group of the highest degree of decline in weight is that of the longest one in life time. 3) In view of increase or decrease of weight ratio in organs, there is some connections of administrating methionin and V B(1) with the decrease in liver and lung at the time of starvation and of administrating olive oil with the decrease in heart. 4) Amylase action of liver at the time of starvation is increased by administerating methionin. 5) Under incomplete starvation by giving mononutriment, the administration of methionin can be able to control the decline of catheptase action of liver. 6) Phosphatase action of kidney at the time of starvation is increased by administrating glucose. 7) In these experiments of starvation, the total amount of urinic nitrogen generally increases before death.
en-copyright=
kn-copyright=

en-aut-name=MatsushimaKuro
en-aut-sei=Matsushima
en-aut-mei=Kuro
kn-aut-name=松島九郎
kn-aut-sei=松島
kn-aut-mei=九郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部法医学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=721
end-page=736
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=INJURY AND EDEMA OF THE TENDON
kn-title=腱の損傷とその浮腫に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Today the statistical study on the edema of the tendon still remains in a state which requires further exploration. Therefore, by using a Mitamura's Abderhalden Dryer and Torsion-Balance, the author studied periodical changes of various edemas evoked by inflicting injuries to tendons, muscles, and skin of rabbits, and obtained the following results: 1. Of the Achilles tendon and long digital flexor tendon sutured groups, the edema both in the silk-thread group of the former and in the stainless-wire group of the latter was found to have reached its maximum stage around the end of the second week after operation, and it disappeared about the end of the sixth week. However, the degree of the edema is higher in the silkthread group than in the stainless-wire group. These findings coincide with microscopic ones. The normal water content of the Achilles tendon is about equal to that of the long digital flexor tendon when the Achilles tendon is severed. 2. Even in the case of severing the Achilles tendon, the edema reaches its maximum around the end of the second week after operation, and it practically disappears about the end of the sixth week. Likewise in the microscopic findings, the cellular substances are most abundant in the second week, showing an irregular inter-fiber spaces. These agree fairly well with the results obtained by Nakura and Takechi. 3. Changes of the edema evoked by pinching of the Achilles tendon, femur muscle, and femur skin take a similar course. The edema caused by the pinching of the musele and skin occurs more rapidly than that by the pinching of the tendon, especially markedly up to 24 hours after operation. 4. In comparing the degrees of the edema, it is highest in the case of pinching the femur muscle, followed by the pinching of the Achilles tendon → in the Achilles tendon sutured with silk thread → the severed part of the Achilles tendon → in the long digital flexor tendon sutured with stainless wire, in the order mentioned.
en-copyright=
kn-copyright=

en-aut-name=HazakiToshihiko
en-aut-sei=Hazaki
en-aut-mei=Toshihiko
kn-aut-name=羽崎寿彦
kn-aut-sei=羽崎
kn-aut-mei=寿彦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部整形外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=715
end-page=719
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Permeabillty of Cell Membrane Ⅱ. Quantitative Estimation of Dye Penetrated in the Cell by Supravital Staining
kn-title=色素の細胞膜透過性について 第二報 顕微分光測光法による透過色素の測定に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By means of microspectrophotometry the author has observed the penetration of the dye qunantitatively on cells stained supravitally with Nile blue. For the quantitative estimation of dye penetrated into the cell the dye in cells was fixed at a certain period of supravital staining by the method devised by Seno, Haba et al, and the dye concentration has been estimated by microspectrophotometry at the wave length of 6000A. Observation revealed that the quantity of penetrated dye in a bone marrow cell at pH 7.0 in one minute was 19.4 x 10(-11) mg in average. The value was found to decrease with the decreased pH of the staining media reaching nearly zero at PH 3.2. In a more acidc media the penetration of the dye increased again but supposed by the destruction of the cell wall. With this method the permeability of dye to one cell in a certain period of time can be estimated exactly in mg. in the range of pH where the destruction of the cell membrane does not occur.
en-copyright=
kn-copyright=

en-aut-name=AmanoIsao
en-aut-sei=Amano
en-aut-mei=Isao
kn-aut-name=天野勲
kn-aut-sei=天野
kn-aut-mei=勲
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部病理学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=709
end-page=714
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Permeability of Cell Membrane Ⅰ. Permeability Test of Cells by Supravital Staining and the Patterns of Paper-electropholesis and Paperchromatography of the Dye in the Varied pH Media
kn-title=色素の細胞膜透過性について 第一報 色素の濾紙電気泳動並に濾紙クロマトグラフィーと腫瘍細胞, 培養細胞を含む種々細胞の超生体染色所見
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A new method of permeability test of cell membrane by supravital staining has been introduced by Seno and Oguchi. Acording to their data the dye permeability to the cell membrane is enchanced in such media as the electric charge of the dye is reduced. But in their experiment the grade of the charge of dye has been observed by electropaperchromatography of dye in a high concentration, 1 percent. The author has found that the electrophoretic picture of dye on a paper is largely dependent upon its concentration, i. e. the size of particle which seems to increase with the reduction of the charge as is clearly seen by paperchromatography. But the test on a diluted dye solution, 0.01 percent, showed that the electrophoresis of dye goes on parallel to the charge of dye or the pH of media, where the paperchromatography gives almost constant picture by changing pH. Using Nile blue the author has observed the permeability of various cells (normal monocytes, white blood cells, bone marrow cells, Yoshida tumor cells, ascites hepatoma cells (AH-130) and L-cells at various pH's (2.2-8.6) and the following results have been obtained. In ascites hepatoma cell (AH-130) and Yoshida tumor cell the permeability to the dye is deeply influenced by the changing pH of media and the depressed permeability till around pH 4.5. The further increase of H ion concentration results in the increased damage of cell with the increased number of the deeply stained cells. L cell also showed the same tendency in the permeability to the dye. The effects of the change of pH on the permeability of the dye were marked on tumor cells and L-cells compared to those on the normal cells.
en-copyright=
kn-copyright=

en-aut-name=AmanoIsao
en-aut-sei=Amano
en-aut-mei=Isao
kn-aut-name=天野勲
kn-aut-sei=天野
kn-aut-mei=勲
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部病理学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2-2
article-no=
start-page=691
end-page=707
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Immuno-serological and Electrophoretic Studies on the Protein of Pregnant Urin Part 2 Electrophoretic Study of the Rabbit Serum Immunized by the Protein of Pregnant Urin
kn-title=妊婦尿蛋白の血清免疫学的ならびに電気泳動学的研究 第2編 妊婦尿蛋白ウサギ免疫血清の電気泳動学的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=1. The total protein density of antiserum, which was immunized with the protein urin of pregnant toxikosis (pregnant edema, pregnant nephrosis, preeclampsia, eclampsia), inflammation of kidney and nephrosis, has increased as compared with before immunization. 2. The total protein density of antiserum absorbed by human serum lessened a little in quantity of antiserum of the urin of pregnant edema and pregnant nephrosis, and the inflammation of kidney which are weak race-speciality (explanation is in Part 1) as compared with that of the urin of preeclampsia, eclampsia, and nephrosis which are strong race-speciality. 3. β-and γ-Globulin where increased by repeated immunization, specially, the rate of γ-Globulin showed a remarkable increase. In case of antiserum of the protein urin of three diseases in strong race-speciality γ-Globulin showed 30.2 % at the maximum amount, 23.8 % at the minimum amount: in case of antiserum of the protein urin of three diseases in weak race-speciality γ-Globulin showed 18.5 % at the maximum amount, which was, however, lower than the minimum amount of the former. In case of antiserum absorbed by human serum, a loss in quantity of β-Globulin in strong race-speciality showed a little increase than that of weak race-speciality, and γ-Globulin showed much increase. 4. The antibody in the antiserum nearly goes side by side with the increase of β-and γ-Globulin. 5. The author thought that the large part of production of antibody were in γ-Globulin, and small part in β-Globulin. α-Globulin increased in antiserum immunized with the protein of only preeclampsia and eclampsia and lessened in antiserum absorbed by human serum. This, the author thinks, will have the connection with the specific protein which was explained in Part 1.
en-copyright=
kn-copyright=

en-aut-name=ItasakaTakuzi
en-aut-sei=Itasaka
en-aut-mei=Takuzi
kn-aut-name=板阪卓児
kn-aut-sei=板阪
kn-aut-mei=卓児
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部法医学教室
END