The ultrastructural and biochemical changes in the brain tissue of 20 mice were studied. The mice, separated into acute and chronic groups, were injected with phorbol myristate acetate (PMA) to trigger the release of free radicals. Malondialdehyde measurement and electron microscopic examination were applied for the evaluation of the effects of the free radicals. The level of lipid peroxide in the chronic PMA group was found to be significantly higher than it was in the acute PMA group (P < 0.005). An electron microscopic examination of the acute group revealed disruption of the mitochondrial cristae and dilatation of the endoplasmic reticulum in the neurons. Myelin sheaths of the nerve fibers exhibited focal structural changes. Neurons and neuroglial cells in the chronic group, however, exhibited distinct ultrastructural alterations. The ultrastructural and biochemical findings showed that free radicals lead to brain damage.
en-copyright= kn-copyright= en-aut-name=BozkirMemducha Gulhal en-aut-sei=Bozkir en-aut-mei=Memducha Gulhal kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=DereFahri en-aut-sei=Dere en-aut-mei=Fahri kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MeteUfuk Ozgu en-aut-sei=Mete en-aut-mei=Ufuk Ozgu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KayaMehmet en-aut-sei=Kaya en-aut-mei=Mehmet kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Cukurova University affil-num=2 en-affil= kn-affil=Cukurova University affil-num=3 en-affil= kn-affil=Cukurova University affil-num=4 en-affil= kn-affil=Cukurova University en-keyword=free radical kn-keyword=free radical en-keyword=phorbol myristate acetate kn-keyword=phorbol myristate acetate en-keyword=malodialdehyde kn-keyword=malodialdehyde en-keyword=brain kn-keyword=brain en-keyword=ultrastruture kn-keyword=ultrastruture END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=4 article-no= start-page=173 end-page=181 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Haptoglobin genotyping by allele-specific polymerase chain reaction amplification en-subtitle= kn-subtitle= en-abstract= kn-abstract=We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp2 = 0.723 and Hp1s = 0.277. Genotyping of Hp was possible with 0.3 ng of DNA and with 0.125 microliter of blood. It was also possible with whole blood left at room temperature for a month and also with the bloodstains left at room temperature for three years. In the heated blood samples, both alleles, Hp2 and Hp1s, were detected in those heated at 100 degrees C for 2 h. In bloodstains, Hp2 and Hp1s were detected in samples heated at 100 degrees C for 2 h and 120 degrees C for 30 min. In addition, the genotype could be detected in samples other than blood such as saliva, hair roots, tissue sections and dental pulps. The present method for Hp genotyping is expected to become a useful method in forensic analysis.
en-copyright= kn-copyright= en-aut-name=YanoAkemi en-aut-sei=Yano en-aut-mei=Akemi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IshizuHideo en-aut-sei=Ishizu en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama Uniiversity en-keyword=DNA polymorphism kn-keyword=DNA polymorphism en-keyword=haptoglobin kn-keyword=haptoglobin en-keyword=polymerase chain reaction kn-keyword=polymerase chain reaction en-keyword=allele-specific amplification kn-keyword=allele-specific amplification en-keyword=personal identification kn-keyword=personal identification END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=4 article-no= start-page=197 end-page=204 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Different expression of Tn and sialyl-Tn antigens between normal and diseased human gastric epithelial cells. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Thomsen-Friedenreich antigen (T antigen) has been supposed to be a cancer-specific carbohydrate antigen. We have previously shown that one third of the Japanese population normally expressed T antigen in gastric surface epithelia and the other two thirds expressed fucosyl-T antigen. Their sialylation and blocked-synthesis were associated with diseased conditions. In the present study, we studied gastric surface epithelial expression of monosaccharide antigen Tn, i.e., a precursor of T antigen, and sialyl-Tn. Normal fundic and pyloric epithelia, respectively, expressed Tn supranucleally and cytoplasmically, but did not express sialyl-Tn. In the intestinal metaplasias and intestinal-type adenomas, goblet cells expressed sialyl-Tn in their vacuoles, and absorptive cells expressed Tn apically. In gastric-type adenomas, Tn, but not sialyl-Tn, was detected. Intestinal-type cancers expressed Tn and sialyl-Tn more often than the diffuse-type cancers. Five cancers did not express Tn, sialyl-Tn, or the T-related antigens. In these, four were diffuse-type cancers. We concluded that: a) normal gastric epithelial cells express Tn; b) metaplastic differentiation is associated with sialylation of Tn and c) expression of Tn and sialyl-Tn is depressed in the gastric cancers.
en-copyright= kn-copyright= en-aut-name=YoshidaAtushi en-aut-sei=Yoshida en-aut-mei=Atushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SotozonoMasaaki en-aut-sei=Sotozono en-aut-mei=Masaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakatouTatsuaki en-aut-sei=Nakatou en-aut-mei=Tatsuaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OkadaYoshio en-aut-sei=Okada en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TsujiTakao en-aut-sei=Tsuji en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama Prefectural University affil-num=5 en-affil= kn-affil=Okayama Prefectural University en-keyword=Tn kn-keyword=Tn en-keyword=immunohistochemistru kn-keyword=immunohistochemistru END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=4 article-no= start-page=189 end-page=196 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Typology of Abdominal Arteries, with Special Reference to Inferior Phrenic Arteries and their Esophageal Branches en-subtitle= kn-subtitle= en-abstract= kn-abstract=Origins and distribution of the human inferior phrenic arteries were studied by dissecting 68 Japanese adult cadavers. The inferior phrenic arteries were usually observed as paired (left and right) vessels. Their origins were summarized as follows: a) the aorta itself (85/138 cases, 61.6%), b) the ventro-visceral arteries (celiaco-mesenteric system of the aorta) including the celiac trunk (39/138 cases, 28.2%) and the left gastric artery (4/138 cases, 2.9%), and c) the latero-visceral arteries (adreno-renal system of the aorta) including the middle adrenal artery (4/138 cases, 2.9%) and the renal artery (6/138 cases, 4.3%). The left and right arteries occasionally originated in common trunk from the aorta, celiaco-mesenteric system or adreno-renal system (22/138 cases, 15.9%). A typological diagram explaining these variations is given. The inferior phrenic arteries, especially the left ones, sometimes issued visceral or esophageal branches. This fact indicates that the inferior phrenic arteries are homologous with the celiac trunk and mesenteric arteries. It is further discussed that the celiac trunk and mesenteric arteries are originally paired vessels, through introduction of our previous typological diagram of the abdominal arteries.
en-copyright= kn-copyright= en-aut-name=PiaoDa Xun en-aut-sei=Piao en-aut-mei=Da Xun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Harbin medical University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=inferior phrenic artery kn-keyword=inferior phrenic artery en-keyword=esophageal branch kn-keyword=esophageal branch en-keyword=typology of abdominal arteries kn-keyword=typology of abdominal arteries END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=4 article-no= start-page=183 end-page=187 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The effect of L-carnitine on platelet activating factor concentration in the immature rat model of hypoxic-ischemic brain injury. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Recent data suggested that platelet-activating factor (PAF) could play a pathophysiologically important role in the progression of hypoxic-ischemic brain injury. We investigated brain tissue PAF concentration in the hypoxic-ischemic brain of immature rats. Endogenous PAF concentration in brain tissue showed a marked increase in hypoxic-ischemic pups (Group 1, 85.6 +/- 15.5 pg/mg protein) when compared to that of the control (9.1 +/- 3.1 pg/mg protein). In addition, we studied the effects of pretreatment with L-carnitine (5 days and 2 h before the hypoxia) on endogenous PAF concentration in the hypoxic-ischemic brain. Endogenous PAF concentration in the short-term pretreatment group (Group 2, 81.6 +/- 9.7 pg/mg protein) was not different than in Group 1 rat pups. However, a significantly decreased PAF concentration was found in the group of pups that received carnitine pretreatment for 5 days (Group 3, 30.5 +/- 11.0 pg/mg protein). These results indicate that PAF is an important mediator in the immature rat model of cerebral hypoxic-ischemic injury. The suppressor effect of L-carnitine on PAF production may give new insight into the treatment of hypoxic-ischemic brain injury.
en-copyright= kn-copyright= en-aut-name=AkisuMete en-aut-sei=Akisu en-aut-mei=Mete kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KultursayNilgun en-aut-sei=Kultursay en-aut-mei=Nilgun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=CokerLsil en-aut-sei=Coker en-aut-mei=Lsil kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HuseyinovAfig en-aut-sei=Huseyinov en-aut-mei=Afig kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Ege University medical School affil-num=2 en-affil= kn-affil=Ege University medical School affil-num=3 en-affil= kn-affil=Ege University medical School affil-num=4 en-affil= kn-affil=Ege University medical School en-keyword=perinatal asphyxia kn-keyword=perinatal asphyxia en-keyword=rat kn-keyword=rat en-keyword=carnitine kn-keyword=carnitine en-keyword=ischmia kn-keyword=ischmia en-keyword=brain kn-keyword=brain en-keyword=platelet-activating factor kn-keyword=platelet-activating factor END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=4 article-no= start-page=225 end-page=231 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Association of fatty liver with increased ratio of visceral to subcutaneous adipose tissue in obese men. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We studied the association of fatty liver with subcutaneous and visceral obesity in 46 male and 36 female patients with body mass index (BMI) over 22 kg/m2. The correlation coefficient between the ratio of the visceral adipose tissue to the subcutaneous adipose tissue (V/S) and the computed tomography (CT) number of the liver was -0.299 (P < 0.05) and that between the V/S ratio and the ratio of the CT number of the liver to that of the spleen (CT-L/CT-S) was -0.335 (P < 0.05) in the males. Partial correlation analysis after making correction for BMI showed an increased correlation coefficient of -0.485 (P < 0.05) between the V/S ratio and the CT-L/CT-S ratio in the males. The odds ratio in the males for CT-L/CT-S below 1.0 and V/S above 1.0 was 3.25 with a 95% confidence interval of 1.02 to 9.39. No such association between the V/S ratio and the CT-L/CT-S ratio was present in the female patients. Multiple regression analysis with serum level of alanine aminotransferase, a marker of fatty liver, as an independent variable revealed a partial regression coefficient of -17.7 for CT-L/CT-S (P < 0.05) in the males and -21.7 (P < 0.05) in the females, validating the CT-L/CT-S ratio as an index of fatty liver. The results indicate the association of fatty liver as determined by the CT-L/CT-S ratio with visceral obesity in males.
en-copyright= kn-copyright= en-aut-name=MahmoodSabina en-aut-sei=Mahmood en-aut-mei=Sabina kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TaketaKazuhisa en-aut-sei=Taketa en-aut-mei=Kazuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ImaiKaori en-aut-sei=Imai en-aut-mei=Kaori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KajiharaYasumasa en-aut-sei=Kajihara en-aut-mei=Yasumasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ImaiShigeki en-aut-sei=Imai en-aut-mei=Shigeki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YokobayashiTsuneo en-aut-sei=Yokobayashi en-aut-mei=Tsuneo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=YamamotoShinichiro en-aut-sei=Yamamoto en-aut-mei=Shinichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=SatoMikio en-aut-sei=Sato en-aut-mei=Mikio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=OmoriHiroyuki en-aut-sei=Omori en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=ManabeKoji en-aut-sei=Manabe en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Kawasaki Medical School Hospital affil-num=5 en-affil= kn-affil=Kawasaki Medical School Hospital affil-num=6 en-affil= kn-affil=Kawasaki Medical School Hospital affil-num=7 en-affil= kn-affil=Kawasaki Medical School Hospital affil-num=8 en-affil= kn-affil=Yura Hospital affil-num=9 en-affil= kn-affil=Shigei Medical Research Hospital affil-num=10 en-affil= kn-affil=Shigei Medical Reseach Hospital en-keyword=fatty liver kn-keyword=fatty liver en-keyword=visceral obesity kn-keyword=visceral obesity en-keyword=computed tomography number kn-keyword=computed tomography number END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=4 article-no= start-page=211 end-page=224 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Evaluation of Rheumatoid Arthritis Using a Scoring System Devised from Magnetic Resonance Imaging of Rheumatoid Knees en-subtitle= kn-subtitle= en-abstract= kn-abstract=We studied the magnetic resonance imaging (MRI) of 120 knees in 86 rheumatoid arthritis (RA) patients and of 14 unaffected knees in 12 control cases. We also developed a scoring system as a quantitative analysis method. We divided the MRI into 10 items, and classified the severity of the symptoms into 4 grades (score 0 to 3). The average total score increased according to the radiographic grade. Soft tissue lesions were clearly detected, even in the early stages of RA. Items such as synovial proliferation showed a high score even in the early stages, suggesting that it was the initial symptom of RA. The score also showed a correlation with the inflammatory signs. These results suggest that this scoring system is very sensitive and yields a good reflection of RA activity. We demonstrated that this system is simple and convenient for routine diagnostic use. We further demonstrated that it is useful for following the advancement of RA and for evaluating the response to treatment.
en-copyright= kn-copyright= en-aut-name=TakeuchiKazuhiro en-aut-sei=Takeuchi en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=InoueHajime en-aut-sei=Inoue en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YokoyamaYoshiki en-aut-sei=Yokoyama en-aut-mei=Yoshiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SendaMasuo en-aut-sei=Senda en-aut-mei=Masuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OtaYusuke en-aut-sei=Ota en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=AbeNobuhiro en-aut-sei=Abe en-aut-mei=Nobuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NishidaKeiichiro en-aut-sei=Nishida en-aut-mei=Keiichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama Universitry affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=rheumatoid arthritis kn-keyword=rheumatoid arthritis en-keyword=magnetic resonance imaging kn-keyword=magnetic resonance imaging en-keyword=scoring system kn-keyword=scoring system en-keyword=synovial membrane kn-keyword=synovial membrane END