Concentrations of homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) were measured in human cerebrospinal fluid (CSF) following long-term storage at -20 degrees C for intervals of three to 60 months. No significant changes in HVA levels were detected in CSF stored for up to 60 months. On the other hand, 5-HIAA concentrations remained stable for up to 6 months, but decreased significantly in the specimens stored for longer time intervals. The results indicate that 5-HIAA should be determined within 6 months after CSF collection, while HVA determinations may be delayed.
en-copyright= kn-copyright= en-aut-name=KobayashiKiyofumi en-aut-sei=Kobayashi en-aut-mei=Kiyofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ImazuYoshihide en-aut-sei=Imazu en-aut-mei=Yoshihide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KawabataMasahiro en-aut-sei=Kawabata en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ShohmoriToshikiyo en-aut-sei=Shohmori en-aut-mei=Toshikiyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Osaka Medical College affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=stability kn-keyword=stability en-keyword=homovanillic acid kn-keyword=homovanillic acid en-keyword=5-hydroxyindoleacetic acid kn-keyword=5-hydroxyindoleacetic acid en-keyword=cerebrospinal fluid kn-keyword=cerebrospinal fluid END start-ver=1.4 cd-journal=joma no-vol=41 cd-vols= no-issue=4 article-no= start-page=145 end-page=154 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=198708 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Concanavalin A-induced cap formation in rat ascites hepatoma cells (AH 7974) and the interaction of cytoplasmic proteins with plasma membranes. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Concanavalin A (Con A) induced cap formation in rat ascites hepatoma cells (AH7974). In these Con A-treated cells, the association of cytoplasmic proteins with cell membranes was suggested by observing their Triton shells. The transition from G-actin to F-actin occurred in these cells. The association of membrane lipid with cytoplasmic proteins extracted from AH cells was studied by the isolation of protein-bound liposomes and phase transition release. The analysis of isolated liposomes revealed that many cytoplasmic proteins which specifically associated with liposomes were cytoskeletal elements including F-actins. The association of proteins with liposomes was affected by the lipid composition of the liposomal membrane and by the Ca2+ concentration of the incubation medium. The strong interaction of liposomal membrane with cytoplasmic proteins or isolated cytoskeletal proteins was demonstrated also by phase transition release using carboxy fluorescein-containing liposomes. These experiments showed that there was a strong affinity between lipid membrane and cytoskeletal elements including F-actins and that the amount of F-actin increased due to Con A treatment. The association of the submembranous microfilaments with the cell membrane may contribute to capping of the cells caused by Con A.
en-copyright= kn-copyright= en-aut-name=MoromizatoYasunori en-aut-sei=Moromizato en-aut-mei=Yasunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WatanabeSadahiro en-aut-sei=Watanabe en-aut-mei=Sadahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=concanavalin A kn-keyword=concanavalin A en-keyword=actins kn-keyword=actins en-keyword=liposomes kn-keyword=liposomes en-keyword=phase trasition release kn-keyword=phase trasition release END start-ver=1.4 cd-journal=joma no-vol=41 cd-vols= no-issue=4 article-no= start-page=173 end-page=178 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=198708 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Histological and Morphological Observation of the Paravestibular Canalicu-lus en-subtitle= kn-subtitle= en-abstract= kn-abstract=The paravestibular canaliculus was studied by light microscopy in serial sections of the temporal bones from otosclerotic patients who underwent fenestration or stapes surgery. In all examined 23 specimens (13 cases), the endolymphatic duct and sac were observed to be normally developed with no pathological findings. The paravestibular canaliculus was found in 14 of the specimens (60.9%). Its course was traced from the proximal to the distal area in 12 specimens, and it appeared to merge into the distal portion of the endolymphatic sac in 7 of them. Twelve of the paravestibular canaliculi contained one vein, and 3 contained several veins. No artery was found. The paravestibular canaliculus was observed to originate from small vascular channels around the vestibule in the otic capsule, lateral to and near the internal aperture of the vestibular aqueduct.
en-copyright= kn-copyright= en-aut-name=MasudaYu en-aut-sei=Masuda en-aut-mei=Yu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FujimotoMasaaki en-aut-sei=Fujimoto en-aut-mei=Masaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OguraYoshio en-aut-sei=Ogura en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ApplebaumEdward L. en-aut-sei=Applebaum en-aut-mei=Edward L. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=University of Illinois en-keyword=temporal bone pathology kn-keyword=temporal bone pathology en-keyword=paravestibular canaliculus kn-keyword=paravestibular canaliculus en-keyword=otosclerosis kn-keyword=otosclerosis END start-ver=1.4 cd-journal=joma no-vol=41 cd-vols= no-issue=4 article-no= start-page=161 end-page=163 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=198708 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Serum antibodies to Escherichia coli in breast-fed and bottle-fed infants. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Titers of antibody against Escherichia coli in human milk and in the sera of 11 breast-fed infants, 6 bottle-fed infants and 9 infants in the post-weaning period were measured by the passive hemagglutination method. High antibody titers were observed in human milk in the first 4 days after parturition, but the titer decreased rapidly thereafter. None of the healthy, breast-fed infants had detectable serum antibodies, while a breast-fed infant with a perianal E. coli abscess had antibodies. On the other hand, 4 of the 6 bottle-fed infants and all of the 9 infants in the post-weaning period had antibodies. The significance of these results was discussed.
en-copyright= kn-copyright= en-aut-name=OguraHideo en-aut-sei=Ogura en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Kochi Medical School en-keyword=breast-fed infant kn-keyword=breast-fed infant en-keyword=bottle-fed infant kn-keyword=bottle-fed infant en-keyword=E. coli antibody kn-keyword=E. coli antibody END start-ver=1.4 cd-journal=joma no-vol=41 cd-vols= no-issue=4 article-no= start-page=155 end-page=160 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=198708 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Synergism between human tumor necrosis factor and human interferon-alpha: effects on cells in culture. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The cytostatic and cytotoxic effects of highly purified natural human tumor necrosis factor (HuTNF-alpha) and natural human interferon-alpha (HuIFN-alpha) on 23 cell lines were studied in vitro. Natural HuTNF-alpha showed cytostatic and cytotoxic effects on PC-9, KHG-2, HT-1197, KG-1 and L-929 cells, and HuIFN-alpha showed both effects on KHG-2 and Daudi cells. A mixture of HuTNF-alpha and HuIFN-alpha (1:1, by unit) showed cytostatic and cytotoxic effects on HuTNF-alpha- or HuIFN-alpha-resistant cell lines such as KB, KATO-III, HEp-2, P-4788, as well as on HuTNF-alpha- or HuIFN-alpha-susceptible cells. Thus, the combined preparation of HuTNF-alpha and HuIFN-alpha expanded the spectrum of sensitive cells. The dosage of the mixed preparation required to produce 50% inhibition of cell growth was less than 20% of that of HuTNF-alpha or HuIFN-alpha alone. These results indicate that the cytostatic and cytotoxic effects of HuTNF-alpha and HuIFN-alpha are synergistically enhanced when they are administered together.
en-copyright= kn-copyright= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AndoShunsaku en-aut-sei=Ando en-aut-mei=Shunsaku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KurimotoMasashi en-aut-sei=Kurimoto en-aut-mei=Masashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Hayashibara Biochemical Laboratories incorporation affil-num=3 en-affil= kn-affil=Hayashibara Biochemical Laboratories Incorporation en-keyword=synergistic enhancement kn-keyword=synergistic enhancement en-keyword=cytostatic effect kn-keyword=cytostatic effect en-keyword=cytotoxic effect kn-keyword=cytotoxic effect en-keyword= HuTNF-? kn-keyword= HuTNF-? en-keyword= HuIFN-? kn-keyword= HuIFN-? END start-ver=1.4 cd-journal=joma no-vol=41 cd-vols= no-issue=4 article-no= start-page=165 end-page=171 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=198708 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Human kidney glomeruli, with special reference to those in the aged person: scanning electron microscopic study of microvascular corrosion casts. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Blood vascular beds of fetal, adult and aged human kidneys were reproduced with methyl methacrylate and observed with a scanning electron microscope. The kidney glomeruli, including those from the fetal kidneys, had anastomosing capillaries. The glomeruli in the kidneys of an aged person contained many more capillaries which were much more tortuous than those of the adult and fetal kidneys. Furthermore, it was observed that the glomeruli in the kidneys of the aged person usually received tortuous afferent vessels and frequently emitted multiple efferent arterioles. The glomeruli in the juxtamedullary layer of the kidneys of the aged person were rather small in size and contained degenerative capillary networks. This observation suggests that the medulla of the kidneys of the aged is poorly supplied with blood.
en-copyright= kn-copyright= en-aut-name=HitomiKusukuma en-aut-sei=Hitomi en-aut-mei=Kusukuma kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KaneshigeTetsuji en-aut-sei=Kaneshige en-aut-mei=Tetsuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=scanning electron microscopy kn-keyword=scanning electron microscopy en-keyword=vascular casts kn-keyword=vascular casts en-keyword=kidney glomerulus kn-keyword=kidney glomerulus en-keyword=aging kn-keyword=aging en-keyword=glomerular efferent vessels kn-keyword=glomerular efferent vessels END start-ver=1.4 cd-journal=joma no-vol=41 cd-vols= no-issue=4 article-no= start-page=183 end-page=185 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=198708 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Fibrillar ultrastructure of the glomerular basement membrane of the rat kidney as revealed by digestive treatment. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Rat kidney glomerular basement membrane (GBM) was isolated and digested with alpha-amylase and elastase. Electron microscopy revealed a meshwork structure composed of fibrils 3 nm in width. They appeared to be type IV collagen fibrils. We succeeded in clarifying a significant ultrastructural aspect of the GBM which had been unclear until now. The findings are consistent with our previously proposed GBM molecular sieve theory.
en-copyright= kn-copyright= en-aut-name=IchiyasuAkira en-aut-sei=Ichiyasu en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakaokaMichio en-aut-sei=Takaoka en-aut-mei=Michio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MakinoHirofumi en-aut-sei=Makino en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakahashiKayo en-aut-sei=Takahashi en-aut-mei=Kayo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OtaZensuke en-aut-sei=Ota en-aut-mei=Zensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=type IV collagen kn-keyword=type IV collagen en-keyword=glomerular basement membrane kn-keyword=glomerular basement membrane en-keyword=enzymatic digestion kn-keyword=enzymatic digestion en-keyword=fibrillar ultrastructure kn-keyword=fibrillar ultrastructure END