The effects of surgical intervention by removal of the primary focus, and the effectiveness of an immunomodulator, Corynebacterium parvum (Cp), on the proliferation of metastatic tumor tissue were investigated by following the postoperative changes in the 3H-thymidine labelling rate of metastatic tissue in an experimental model of metastasis in mice. In addition, the delayed type hypersensitivity reaction (DTH) was studied to investigate the immune capacity of the host. The labelling rate of mice that had the primary focus removed remained high with little variation, while that of the mice not operated on decreased gradually. On the other hand, in mice undergoing a sham operation, the rate was the same as that of the mice with the primary focus removed for a short while, but then gradually decreased. When Cp was administered, especially before removal of the primary focus, the rate was lower than that of the tumor bearing control group and decreased steadily. The number of pulmonary metastatic nodules was increased by removal of the primary focus, but this increase was inhibited by the administration of Cp which prolonged life. The depression in the DTH was less in the group given Cp preoperativeLy than in either the group of mice having the primary focus removed or those not having it removed.
en-copyright= kn-copyright= en-aut-name=HashimotoOsamu en-aut-sei=Hashimoto en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiwaHiroaki en-aut-sei=Miwa en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=metastatic tumor kn-keyword=metastatic tumor en-keyword=surgical intervension kn-keyword=surgical intervension en-keyword=autoradiography kn-keyword=autoradiography en-keyword=Corynebacterium parvum kn-keyword=Corynebacterium parvum END start-ver=1.4 cd-journal=joma no-vol=37 cd-vols= no-issue=4 article-no= start-page=367 end-page=376 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=198308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Dissociation of natural killer activity and antibody-dependent cellular cytotoxicity in spleen cells of tumor bearing mice. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Antibody-dependent cellular cytotoxicity (ADCC) increased with the development of tumors in C3H/He mice bearing spontaneous breast cancer or the syngeneic hepatoma MH-134 and in C57BL/6 mice bearing the syngeneic Lewis lung carcinoma 3LL. This cytotoxicity decreased after treatment with guinea pig, monoclonal IgM anti-Thy 1.2 serum and complement to the non-cancer level thus indicating that the increased ADCC in mice with cancer seems mainly attributable to cells with the Thy 1 antigen. On the other hand, NK activity decreased greatly when mice had tumors. Treatment with monoclonal IgM anti-Thy 1.2 serum and complement showed no significant influence on the natural killer (NK) activity of spleen cells of mice bearing MH-134 cancer, but in the 3LL-bearing mice the activity decreased significantly.
en-copyright= kn-copyright= en-aut-name=OnoMinoru en-aut-sei=Ono en-aut-mei=Minoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=ADCC acitivity kn-keyword=ADCC acitivity en-keyword=NK activity kn-keyword=NK activity en-keyword=Thy 1 antigen kn-keyword=Thy 1 antigen en-keyword=plastic dish fractionation kn-keyword=plastic dish fractionation en-keyword=tumor-bearing mice kn-keyword=tumor-bearing mice END start-ver=1.4 cd-journal=joma no-vol=37 cd-vols= no-issue=4 article-no= start-page=381 end-page=384 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=198308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Serum neutral amino acid concentrations in cirrhotic patients with impaired carbohydrate metabolism. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Serum neutral amino acid levels in cirrhotic patients with abnormal oral glucose tolerance test patterns were not different from those of subjects without impaired carbohydrate metabolism. However, the characteristic features of serum aminograms in the patients, that is, increased levels of tyrosine, decreased levels of valine and leucine and the diminished ratio of branched chain amino acids to phenylalanine and tyrosine levels, were less pronounced in those treated with insulin. This finding is clinically important for evaluating the serum aminogram of cirrhotic patients under insulin therapy.
en-copyright= kn-copyright= en-aut-name=WatanabeAkiharu en-aut-sei=Watanabe en-aut-mei=Akiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakeiNobuyuki en-aut-sei=Takei en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HayashiSyosaku en-aut-sei=Hayashi en-aut-mei=Syosaku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=amino acid kn-keyword=amino acid en-keyword=cirrhotics kn-keyword=cirrhotics en-keyword=carbohydrate metabolism kn-keyword=carbohydrate metabolism en-keyword=insulin kn-keyword=insulin en-keyword=glucose tolerance test kn-keyword=glucose tolerance test END start-ver=1.4 cd-journal=joma no-vol=37 cd-vols= no-issue=4 article-no= start-page=273 end-page=282 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=198308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Restriction of host range of xenotropic pseudotype murine sarcoma virus by helper leukemia virus. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We investigated the restriction of the host range to infectivity of MSV by helper leukemia virus in vivo. When newborn SD-rats were inoculated intracerebrally, subcutaneously, intraperitoneally or intramuscularly with xenotropic pseudotype Kirsten MSV, Ki-MSV(BV2), either brain tumors or myogenic sarcomas were induced, depending upon the route of inoculation. However, no tumors developed in SW-Icr mice inoculated with Ki-MSV(BV2) either intracerebrally or intramuscularly at birth. Ecotropic Ki-MSV(Ki-MuLV) induced myogenic sarcomas in mice when inoculated intramuscularly and also induced brain tumors and myogenic sarcomas in rats when inoculated intracerebrally and intramuscularly, respectively. Thus, the host range of pseudotype MSV appeared to depend on a helper leukemia virus.
en-copyright= kn-copyright= en-aut-name=OkazakiTomio en-aut-sei=Okazaki en-aut-mei=Tomio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=host range kn-keyword=host range en-keyword=helper leukemia virus kn-keyword=helper leukemia virus en-keyword=pseudotype MSV kn-keyword=pseudotype MSV END start-ver=1.4 cd-journal=joma no-vol=37 cd-vols= no-issue=4 article-no= start-page=321 end-page=333 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=198308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of a branched chain amino acid-enriched nutritional product on the pathophysiology of the liver and nutritional state of patients with liver cirrhosis. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A new nutritional product (SF-1008C) containing a high proportion of branched chain amino acids (BCAA) and low proportion of aromatic amino acids (AAA) and methionine was tested to see its effect on the impaired protein metabolism and abnormal nutritional state frequently observed in patients with advanced liver cirrhosis. A sharp increase in plasma BCAA levels and fall of AAA and methionine levels were found following the administration of an SF-1008C-supplemented diet to healthy controls and cirrhotic patients, which the BCAA levels increased only slightly following an isocaloric control diet. Blood ammonia levels increased within the normal range transiently following the diets. The SF-1008C-supplemented diet was given for 2 weeks to cirrhotic patients with histories of hepatic encephalopathy, who were taking a low-protein diet because of hyperammonemia. Serum prealbumin levels, nitrogen balance, molar ratio of plasma BCAA/phenylalanine and tyrosine, the number connection test and electroencephalograms improved during the period of the experimental diet. The results, therefore, indicate that a BCAA-supplemented diet is well tolerated by patients with advanced cirrhosis and useful for treatment of impaired protein metabolism. Furthermore, this product is beneficial in preventing hepatic encephalopathy in cirrhotics.
en-copyright= kn-copyright= en-aut-name=WatanabeAkiharu en-aut-sei=Watanabe en-aut-mei=Akiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShiotaTetsuya en-aut-sei=Shiota en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OkitaMisako en-aut-sei=Okita en-aut-mei=Misako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NagashimaHideo en-aut-sei=Nagashima en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=branched chain amino acid kn-keyword=branched chain amino acid en-keyword=aromatic amino acid kn-keyword=aromatic amino acid en-keyword=nutritional product kn-keyword=nutritional product en-keyword=liver cirrhosis kn-keyword=liver cirrhosis en-keyword=hepatic encephalopathy kn-keyword=hepatic encephalopathy END start-ver=1.4 cd-journal=joma no-vol=37 cd-vols= no-issue=4 article-no= start-page=301 end-page=320 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=198308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Tissue Culture of Trigeminal Nerves from Rats Administered Transplacentally with Ethylnitrosourea en-subtitle= kn-subtitle= en-abstract= kn-abstract=The morphological and biological changes in long term culture cells of normal-appearing trigeminal nerves from 2, 8, and 30-day-old S-D rats administered transplacentaLly with 75 mg ENU/kg were examined. After a marked degeneration of cells, crisscross multiple proliferative foci of transformed spindle cells appeared at the 3rd passage culture from 2 and 8-day-old rats, but not form 30-day-old rats. The transformed cells with S-100 protein and basal lamina had Schwann cell characteristics. Transformed spindle cells continued to form a crisscross pattern more than 700 days and some transformed spindle cells became round in shape 3-6 months after the primary culture. These transformed cells were transplantable to newborn S-D rats and the transplanted tumors were histologically similar to those of malignant Schwannoma of trigeminal nerves induced by ENU. Round-shaped transformed cells were more malignant than spindle-shaped cells and produced rapidly growing transplanted tumors. Spontaneous transformation with multinucleated giant cells occurred in one of the control cultures. These results indicate that the sequential changes of ENU-treated trigeminal nerves in vitro were corresponded to developmental changes of malignant Schwannoma in vivo induced by ENU. This system will be useful for analysis of ENU-carcinogenesis.
en-copyright= kn-copyright= en-aut-name=HayashiKazuhiko en-aut-sei=Hayashi en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=experimental malignant Schwannoma kn-keyword=experimental malignant Schwannoma en-keyword=trigeminal nerves of rat kn-keyword=trigeminal nerves of rat en-keyword=ENU kn-keyword=ENU en-keyword=in vitro transformation kn-keyword=in vitro transformation en-keyword=spontaneous transformation kn-keyword=spontaneous transformation END start-ver=1.4 cd-journal=joma no-vol=37 cd-vols= no-issue=4 article-no= start-page=377 end-page=380 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=198308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Elicitation of cyclic AMP accumulation by norepinephrine in the iron-induced epileptogenic cerebral cortex of rats. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A microinjection of ferric chloride solution into the left frontal cortex of rats induced epileptic discharges which were recorded by electrocorticography. In animals having such electrographic seizure activity 30 to 60 days after the injection, the accumulation of cyclic AMP elicited by norepinephrine was examined in slices from four cortical regions. The accumulation was significantly greater in the left anterior area, into which region the ferric chloride solution was injected, than in the right anterior area. There was also a tendency for greater norepinephrine-elicited accumulation of cyclic AMP to occur in the left posterior area than in the right posterior area.
en-copyright= kn-copyright= en-aut-name=HattoriYukio en-aut-sei=Hattori en-aut-mei=Yukio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MoriwakiAkiyoshi en-aut-sei=Moriwaki en-aut-mei=Akiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YasuharaHiromichi en-aut-sei=Yasuhara en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NishidaNobuyoshi en-aut-sei=Nishida en-aut-mei=Nobuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HoriYasuo en-aut-sei=Hori en-aut-mei=Yasuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=rat cerebral cortex kn-keyword=rat cerebral cortex en-keyword=iron-induced epileptic discharge kn-keyword=iron-induced epileptic discharge en-keyword=cortical slices kn-keyword=cortical slices en-keyword=cyclic AMP kn-keyword=cyclic AMP en-keyword=norepinephrine kn-keyword=norepinephrine END start-ver=1.4 cd-journal=joma no-vol=37 cd-vols= no-issue=4 article-no= start-page=291 end-page=299 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=198308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Dual Effects of Glutamate on Cyclic AMP Levels in Slices of Rat Cerebral Cortex with an Iron-Induced Epireptic Focus en-subtitle= kn-subtitle= en-abstract= kn-abstract=An epileptic focus not resulting in generalized convulsions was induced by a microinjection of ferric chloride solution into the left anterior cortex of rats. The formation of the epileptic focus was confirmed by the appearance of bilateral spike and slow wave complexes as well as focal isolated spikes in electrocorticograms (ECoGs). The effect of glutamate on cyclic AMP accumulation was examined in incubated slices prepared from four quadrants of the epileptic cortex. In animals showing isolated spikes 8 to 10 days after the microinjection, the effect of glutamate on cyclic AMP accumulation was stimulatory. It was greatest in the left anterior quadrant which included the injection site, but only slight in the left and right posterior quadrants. In animals showing spike and slow wave complexes 30 to 60 days after the microinjection, the stimulatory effect of glutamate was also most pronounced in the left anterior quadrant. In the right anterior and the left posterior quadrants glutamate had almost no effect, while in the right posterior quadrant, glutamate was inhibitory.
en-copyright= kn-copyright= en-aut-name=HattoriYukio en-aut-sei=Hattori en-aut-mei=Yukio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=InabaKozo en-aut-sei=Inaba en-aut-mei=Kozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MoriwakiAkiyoshi en-aut-sei=Moriwaki en-aut-mei=Akiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NishidaNobuyoshi en-aut-sei=Nishida en-aut-mei=Nobuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YasuharaHiromichi en-aut-sei=Yasuhara en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HoriYasuo en-aut-sei=Hori en-aut-mei=Yasuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=rat cerebral cortex kn-keyword=rat cerebral cortex en-keyword=iron-induced epileptic focus kn-keyword=iron-induced epileptic focus en-keyword=cortical slices kn-keyword=cortical slices en-keyword=glutamate kn-keyword=glutamate en-keyword=cyclic AMP kn-keyword=cyclic AMP END start-ver=1.4 cd-journal=joma no-vol=37 cd-vols= no-issue=4 article-no= start-page=283 end-page=289 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=198308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Susceptibility of Rous sarcoma virus-specific sequences integrated into SR-C3H/He mouse ascites sarcoma cell chromatin to DNase I and DNase II. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The susceptibility of Rous sarcoma virus (RSV) genomes integrated in mouse ascites sarcoma cells (SR-C3H/He cells) to DNase I and DNase II was investigated. Approximately half of the viral sequences were sensitive to DNase I and DNase II when 17% and 7.4% of the chromatin DNA was rendered acid soluble, respectively. The results suggest that newly acquired exogenous proviral sequences are integrated into both transcriptionally active and inactive regions of chromatin in cells lacking related endogenous viral sequences.
en-copyright= kn-copyright= en-aut-name=TstsuiKimiko en-aut-sei=Tstsui en-aut-mei=Kimiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsutsuiKen en-aut-sei=Tsutsui en-aut-mei=Ken kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=proviral sequences kn-keyword=proviral sequences en-keyword=mouse ascites sarcoma cells kn-keyword=mouse ascites sarcoma cells en-keyword=chromatin kn-keyword=chromatin en-keyword=deoxyribonucleases kn-keyword=deoxyribonucleases END start-ver=1.4 cd-journal=joma no-vol=37 cd-vols= no-issue=4 article-no= start-page=353 end-page=366 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=198308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Immunocytochemical localization of T antigen in cells of BK virus-induced hamster brain tumor. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The localization of both the large T and small t tumor (T) antigens in cultured cells (Vn 12 cells) of hamster brain tumors induced with BK virus (BKV), a new human papovavirus, was studied by an enzyme labelled antibody method at both the light and electron microscopic levels. Under the light microscope, BKV T antigen was observed in the nucleus, except for the nucleoli, of cells in interphase, and under the electron microscope it was observed in the nucleus except for the nucleoli and nuclear membrane. BKV T antigen appears to be closely associated with nuclear chromatin as previously reported for simian virus 40 tumor antigen (SV40 T antigen). The intracellular localization of BKV T antigen was the same as that of SV40 T antigen. In metaphase, BKV T antigen seems to be distributed diffusely throughout the cytoplasm except for the chromosomes. In telophase, BKV T antigen transfers from the cytoplasm to the nucleus. The migration of BKV T antigen during the cell cycle is thought to be related to the function of T antigen.
en-copyright= kn-copyright= en-aut-name=TsuboiMasahiro en-aut-sei=Tsuboi en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=experimental brain tumor kn-keyword=experimental brain tumor en-keyword=BK virus kn-keyword=BK virus en-keyword=human papovavirus kn-keyword=human papovavirus en-keyword=T antigen kn-keyword=T antigen en-keyword=immunocytochemistry kn-keyword=immunocytochemistry END start-ver=1.4 cd-journal=joma no-vol=37 cd-vols= no-issue=4 article-no= start-page=341 end-page=352 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=198308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Primary cultured cells and an established cell line of human hepatocellular carcinomas. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Cancer cells obtained from human hepatocellular carcinoma nodules were subjected to primary culture, and a hepatoma cell line was established. The cell clumps obtained by needle puncture were plated directly in plastic tissue culture flasks without any cell dissociation procedures. Cell clusters became attached to flasks in 24 h with an efficiency of about 90%. No fibroblast outgrowth was observed. Primary cultured cells were composed of polygonally shaped epithelial cells with dense cytoplasm and one or more large nuclei. They excreted plasma protein biosynthetic markers of hepatocytes into the culture medium. Plasma protein synthesis of primary cultured hepatoma cells decreased as the age of the primary cultures increased. Cells seeded in September 1980 started to grow continuously after 5 months of cultivation. A new hepatocellular carcinoma cell line (designated as KG55T) was established from these growing cells. KG55T cells have been subcultured for more than 20 passages and form a monolayer of polygonal epithelial cells which pile up after they reach confluence. The cells had a doubling time of 50-60 h and a plating efficiency of 60-65%. Albumin, alpha 1-antitrypsin and alpha 2-macroglobulin syntheses and tyrosine aminotransferase activity were detected. At the 10th passage, KG55T cells were pseudotriploid (mode, 69), and 8q+ and 15q+ translocations were distinctive of this cell line. The morphological characteristics and the capacity for plasma protein synthesis of the primary cultured hepatoma cells and cells of the established hepatoma cell line were compared.
en-copyright= kn-copyright= en-aut-name=MatsuuraHidemitsu en-aut-sei=Matsuura en-aut-mei=Hidemitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=primary culture kn-keyword=primary culture en-keyword=human hepatoma cell line kn-keyword=human hepatoma cell line en-keyword=plasma protein kn-keyword=plasma protein END