start-ver=1.4
cd-journal=joma
no-vol=45
cd-vols=
no-issue=11
article-no=
start-page=3322
end-page=3337
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220907
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=FE UPTAKE‐INDUCING PEPTIDE1 maintains Fe translocation by controlling Fe deficiency response genes in the vascular tissue of Arabidopsis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=FE UPTAKE-INDUCING PEPTIDE1 (FEP1), also named IRON MAN3 (IMA3) is a short peptide involved in the iron deficiency response in Arabidopsis thaliana. Recent studies uncovered its molecular function, but its physiological function in the systemic Fe response is not fully understood. To explore the physiological function of FEP1 in iron homoeostasis, we performed a transcriptome analysis using the FEP1 loss-of-function mutant fep1-1 and a transgenic line with oestrogen-inducible expression of FEP1. We determined that FEP1 specifically regulates several iron deficiency-responsive genes, indicating that FEP1 participates in iron translocation rather than iron uptake in roots. The iron concentration in xylem sap under iron-deficient conditions was lower in the fep1-1 mutant and higher in FEP1-induced transgenic plants compared with the wild type (WT). Perls staining revealed a greater accumulation of iron in the cortex of fep1-1 roots than in the WT root cortex, although total iron levels in roots were comparable in the two genotypes. Moreover, the fep1-1 mutation partially suppressed the iron overaccumulation phenotype in the leaves of the oligopeptide transporter3-2 (opt3-2) mutant. These data suggest that FEP1 plays a pivotal role in iron movement and in maintaining the iron quota in vascular tissues in Arabidopsis.
en-copyright=
kn-copyright=

en-aut-name=OkadaSatoshi
en-aut-sei=Okada
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=LeiGui J.
en-aut-sei=Lei
en-aut-mei=Gui J.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YamajiNaoki
en-aut-sei=Yamaji
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HuangSheng
en-aut-sei=Huang
en-aut-mei=Sheng
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MaJian F.
en-aut-sei=Ma
en-aut-mei=Jian F.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MochidaKeiichi
en-aut-sei=Mochida
en-aut-mei=Keiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=HirayamaTakashi
en-aut-sei=Hirayama
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Group of Environmental Stress Response Systems, Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=2
en-affil=Group of Plant Stress Physiology, Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=3
en-affil=Group of Plant Stress Physiology, Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=4
en-affil=Group of Plant Stress Physiology, Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=5
en-affil=Group of Plant Stress Physiology, Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=6
en-affil=Crop Design Research Team, Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=7
en-affil=Group of Environmental Stress Response Systems, Institute of Plant Science and Resources, Okayama University
kn-affil=
en-keyword=oestrogen induction system
kn-keyword=oestrogen induction system
en-keyword=fep1-1
kn-keyword=fep1-1
en-keyword=iron-deficiency response
kn-keyword=iron-deficiency response
en-keyword=transcriptome
kn-keyword=transcriptome
END