フルテキストURL J_Comp_Neurol_525_7_1586.pdf
著者 Tamura, Kei| Kobayashi, Yasuhisa| Hirooka, Asuka| Takanami, Keiko| Oti, Takumi| Jogahara, Takamichi| Oda, Sen-ichi| Sakamoto, Tatsuya| Sakamoto, Hirotaka|
抄録 Several regions of the brain and spinal cord control male reproductive function. We previously demonstrated that the gastrin-releasing peptide (GRP) system, located in the lumbosacral spinal cord of rats, controls spinal centers to promote penile reflexes during male copulatory behavior. However, little information exists on the male-specific spinal GRP system in animals other than rats. The objective of this study was to examine the functional generality of the spinal GRP system in mammals using the Asian house musk shrew (Suncus murinus; suncus named as the laboratory strain), a specialized placental mammal model. Mice are also used for a representative model of small laboratory animals. We first isolated complementary DNA encoding GRP in suncus. Phylogenetic analysis revealed that suncus preproGRP was clustered to an independent branch. Reverse transcription-PCR showed that GRP and its receptor mRNAs were both expressed in the lumbar spinal cord of suncus and mice. Immunohistochemistry for GRP demonstrated that the sexually dimorphic GRP system and male-specific expression/distribution patterns of GRP in the lumbosacral spinal cord in suncus are similar to those of mice. In suncus, we further found that most GRP-expressing neurons in males also express androgen receptors, suggesting that this male-dominant system in suncus is also androgen-dependent. Taken together, these results indicate that the sexually dimorphic spinal GRP system exists not only in mice but also in suncus, suggesting that this system is a conserved property in mammals.
キーワード RRID AB_2060157 RRID: AB_2571636 RRID: AB_626757 Suncus murinus (suncus) gastrin-releasing peptide male reproductive function sexual dimorphism spinal cord
備考 This is the peer reviewed version of the following article: Tamura K. Kobayashi Y. Hirooka A. et al. Identification of the sexually dimorphic gastrin-releasing peptide system in the lumbosacral spinal cord that controls male reproductive function in the mouse and Asian house musk shrew (Suncus murinus). J Comp Neurol. 2017;525:1601–1613. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. 2018年5月公開予定
発行日 2017-05-01
出版物タイトル Journal of Comparative Neurology
525巻
7号
出版者 Wistar Institute of Anatomy and Biology
開始ページ 1586
終了ページ 1598
ISSN 0021-9967
NCID AA00695917
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
論文のバージョン author
PubMed ID 27804131
Web of Sience KeyUT 000397559700004
関連URL https://doi.org/10.1002/cne.24138