ID | 52814 |
フルテキストURL | |
著者 |
Monden, Yuki
Graduate School of Environmental and Life Science, Okayama University
Kaken ID
publons
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Takasaki, Kazuto
FASMAC Co., Ltd.
Futo, Satoshi
FASMAC Co., Ltd.
Niwa, Kousuke
Graduate School of Biomedical Engineering, Tohoku University
Kawase, Mitsuo
Graduate School of Biomedical Engineering, Tohoku University
Akitake, Hiroto
Graduate School of Environmental and Life Science, Okayama University
Tahara, Makoto
Graduate School of Environmental and Life Science, Okayama University
Kaken ID
publons
researchmap
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抄録 | In many crops species, the development of a rapid and precise cultivar discrimination system has been required for plant breeding and patent protection of plant cultivars and agricultural products. Here, we successfully evaluated strawberry cultivars via a novel method, namely, the single tag hybridization (STH) chromatographic printed array strip (PAS) using the PCR products of eight genomic regions. In a previous study, we showed that genotyping of eight genomic regions derived from FaRE1 retrotransposon insertion site enabled to discriminate 32 strawberry cultivars precisely, however, this method required agarose/acrylamide gel electrophoresis, thus has the difficulty for practical application. In contrast, novel DNA detection method in this study has some great advantages over standard DNA detection methods, including agarose/acrylamide gel electrophoresis, because it produces signals for DNA detection with dramatically higher sensitivity in a shorter time without any preparation or staining of a gel. Moreover, this method enables the visualization of multiplex signals simultaneously in a single reaction using several independent amplification products. We expect that this novel method will become a rapid and convenient cultivar screening assay for practical purposes, and will be widely applied to various situations, including laboratory research, and on-site inspection of plant cultivars and agricultural products.
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キーワード | Cultivar discrimination
Multiplex PCR
Strawberry
Practical application
Retrotransposon
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発行日 | 2014-09-20
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出版物タイトル |
Journal of Biotechnology
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巻 | 185巻
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出版者 | Elsevier B.V.
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開始ページ | 57
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終了ページ | 62
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ISSN | 0168-1656
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資料タイプ |
学術雑誌論文
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言語 |
英語
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著作権者 | © 2014 The Authors. Published by Elsevier B.V.
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論文のバージョン | publisher
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査読 |
有り
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DOI |