start-ver=1.4
cd-journal=joma
no-vol=156
cd-vols=
no-issue=1-2
article-no=
start-page=82
end-page=93
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=201312
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=IL-7 promotes long-term in vitro survival of unique long-lived memory subset generated from mucosal effector memory CD4(+) T cells in chronic colitis mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Colitogenic memory CD4(+) T cells are important in the pathogenesis of inflammatory bowel disease (IBD). Although memory stem cells with high survival and self-renewal capacity were recently identified in both mice and humans, it is unclear whether a similar subset is present in chronic colitis mice. We sought to identify and purify a long-lived subset of colitogenic memory CD4(+) T cells, which may be targets for treatment of IBD. A long-lived subset of colitogenic memory CD4(+) T cells was purified using a long-term culture system. The characteristics of these cells were assessed. Interleukin (IL)-7 promoted the in vitro survival for >8 weeks of lamina propria (LP) CD4(+) T cells from colitic SOD mice previously injected with CD4(+)CD45RB(high) T cells. These cells were in a quiescent state and divided a maximum of 5 times in 4 weeks. LP CD4(+) T cells expressed higher levels of Bcl-2, integrin-alpha 4 beta 7, CXCR3 and CD25 after than before culture, as well as secreting high concentrations of IL-2 and low concentrations of IFN-gamma and IL-17 in response to intestinal bacterial antigens. LP CD4(+) T cells from colitic mice cultured with IL-7 for 8 weeks induced more severe colitis than LP CD4(+) T cells cultured for 4 weeks. We developed a novel culture system to purify a long-lived, highly pathogenic memory subset from activated LP CD4(+) T cells. IL-7 promoted long-term in vitro survival of this subset in a quiescent state. This subset will be a novel, effective target for the treatment of IBD.
en-copyright=
kn-copyright=

en-aut-name=TakaharaMasahiro
en-aut-sei=Takahara
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NemotoYasuhiro
en-aut-sei=Nemoto
en-aut-mei=Yasuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OshimaShigeru
en-aut-sei=Oshima
en-aut-mei=Shigeru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MatsuzawaYu
en-aut-sei=Matsuzawa
en-aut-mei=Yu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KanaiTakanori
en-aut-sei=Kanai
en-aut-mei=Takanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=OkamotoRyuichi
en-aut-sei=Okamoto
en-aut-mei=Ryuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TsuchiyaKiichiro
en-aut-sei=Tsuchiya
en-aut-mei=Kiichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=NakamuraTetsuya
en-aut-sei=Nakamura
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=YamamotoKazuhide
en-aut-sei=Yamamoto
en-aut-mei=Kazuhide
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=WatanabeMamoru
en-aut-sei=Watanabe
en-aut-mei=Mamoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

affil-num=1
en-affil=
kn-affil=Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch

affil-num=2
en-affil=
kn-affil=Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch

affil-num=3
en-affil=
kn-affil=Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch

affil-num=4
en-affil=
kn-affil=Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch

affil-num=5
en-affil=
kn-affil=Keio Univ, Dept Internal Med, Div Gastroenterol & Hepatol, Sch Med

affil-num=6
en-affil=
kn-affil=Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch

affil-num=7
en-affil=
kn-affil=Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch

affil-num=8
en-affil=
kn-affil=Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch

affil-num=9
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Gastroenterol & Hepatol

affil-num=10
en-affil=
kn-affil=Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
en-keyword=Inflammatory bowel disease
kn-keyword=Inflammatory bowel disease
en-keyword=Memory T cells
kn-keyword=Memory T cells
en-keyword=CD4
kn-keyword=CD4
en-keyword=IL-7
kn-keyword=IL-7
en-keyword=Cell culture
kn-keyword=Cell culture
END