In the area of biotechnology, a micromanipulation is widely used for such purposes as operating on genes and transferring biological materials into cells. For the some experiments, such as biochemical experiment, a large number of cells have to be manipulated in a short time. We have developed an automatic micromanipulation system under the stereoscopic microscope. Micromanipulation system carries out various processes, such as detection of the target, the detection of the needle head, and motor control. By sharing these processes with several computers, the micromanipulation can be performed at high speed. As a result, computer cooperation becomes very important. In this paper, we propose a multi agent micromanipulation system. At first, we developed a multi agent system, which performs image processing, motor control, and management of the micromanipulation processes. Secondarily, we proposed to operate computers cooperative. We use a computer as a single agent. And several computers are connected to a local area network. The multi agent micromanipulation system performed the micromanipulation at a realistic rate through cooperation of multi agents.
en-copyright= kn-copyright= en-aut-name=SanoTetsuya en-aut-sei=Sano en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamamotoHideki en-aut-sei=Yamamoto en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=Micromanipulation kn-keyword=Micromanipulation en-keyword=Stereoscopic Microscope kn-keyword=Stereoscopic Microscope en-keyword=Stereo vision kn-keyword=Stereo vision en-keyword=Image processing kn-keyword=Image processing END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page=48 end-page=52 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20045 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Micromanipulation with Stereoscopic Imaging en-subtitle= kn-subtitle= en-abstract= kn-abstract=The neuronal organization of the hippocampus is extensively studied. However, the synoptic connections between neurons of the hilus and neurons of the granule cell layer are still debate. In the present study, we utilized automated micromanipulation technique combined with visual image processing to analyze the synaptic connections between hilar mossy cells or mossy fibers, and granule cells or basket cells in the granule cell layer. The stereoscopic microscope image of the dentate gyms of coronal slice in adult rat brain was converted into a binary image. The area of the dentate gyms is detected by genetic algorithms. This was matched with the template image made from a microscopic photo of cresyl violet-stained coronal section of the rat brain by parallel and rotation matching. A glass microelectrode inserted into the apex of the dentate gyrus by the micromanipulation system. Lipophilic fluorescent tracer DiI or DiD was injected into the hilus by a nanoinjector.
en-copyright= kn-copyright= en-aut-name=SanoTetsuya en-aut-sei=Sano en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamamotoHideki en-aut-sei=Yamamoto en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=Image Processing kn-keyword=Image Processing en-keyword=Micromanipulation kn-keyword=Micromanipulation en-keyword=Neuronal Organization kn-keyword=Neuronal Organization en-keyword=Microinjection kn-keyword=Microinjection END