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ID 49038
フルテキストURL
著者
Snyderwine, Elizabeth G. Laboratory of Experimental Carcinogenesis, National Cancer Institute Bethesda
Davis, Cindy D. Laboratory of Experimental Carcinogenesis, National Cancer Institute Bethesda
Nouso, Kazuhiro Laboratory of Experimental Carcinogenesis, National Cancer Institute Bethesda ORCID Kaken ID publons researchmap
RoIler, Peter P. Laboratory of Medicinal Chemistry, National Cancer Institute Bethesda
Schut, Herman A.J. Department of Pathology, Medical College of Ohio Toledo
抄録
The P-32-postlabeling method was used to examine the adducts in DNA, polynucleotides, and mononucleotides reacted in vitro with the N-hydroxy and N-acetoxy derivatives of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3, 8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) or 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Adduct profiles were compared to those found in vivo in liver of cynomolgus monkeys fed IQ, MeIQx or PhIP. The N-acetoxy derivatives of IQ, MeIQx and PhIP (generated in situ from the corresponding N-hydroxylamine in the presence of acetic anhydride) each formed three principal adducts in DNA. Adduct 1 of IQ, MeIQx and PhIP was chromatographically identical to the P-32-labeled bis(phosphate) derivative of N-(deoxyguanosin-8-yl)-IQ, N-(deoxyguanosin-8-yl)-MeIQx, and N-(deoxyguanosin-8-yl)-PhIP respectively, and this adduct comprised approximately 65% of total adduct levels found in DNA in vitro. The C8-guanine adduct and the two minor adducts were also found in poly(dG-dC).poly(dG-dC), suggesting that the two minor adducts of IQ, MeIQx and PhIP are also formed on the guanine base. The N-acetoxy derivatives of IQ, MeIQx, and to a much lesser extent PhIP, also formed adducts with adenine-containing polynucleotides including poly(dA), poly(dA).poly(dT) and poly(dA-dT).poly(dA-dT), but these adenine adducts were chromatographically different from those found in DNA. The three guanine adducts of N-acetoxy-IQ, -MeIQx and -PhIP found in vitro in DNA and in guanine-containing polynucleotides were also found in the liver of monkeys fed IQ, MeIQx or PhIP respectively, indicating that metabolic activation via N-hydroxylation and esterification occurred in vivo in monkeys. With each compound, the C8-guanine adduct was the predominant adduct found in vivo. The results indicate similarities among IQ, MeIQx and PhIP in the DNA adducts formed in vitro and in vivo and substantiate the use of the P-32-postlabeling method for comparative adduct studies.
発行日
1993-07
出版物タイトル
Carcinogenesis
14巻
7号
開始ページ
1389
終了ページ
1395
ISSN
0143-3334
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1093/carcin/14.7.1389
言語
英語
論文のバージョン
publisher
査読
有り
DOI
Web of Science KeyUT