start-ver=1.4
cd-journal=joma
no-vol=82
cd-vols=
no-issue=7
article-no=
start-page=1172
end-page=1175
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=201804
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Novel assay system for acidic Peptide:N-glycanase (aPNGase) activity in crude plant extract
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Acidic peptide:N-glycanase (aPNGase) plays a pivotal role in plant glycoprotein turnover. For the construction of aPNGase-knockout or -overexpressing plants, a new method to detect the activity in crude plant extracts is required because endogenous peptidases present in the extract hamper enzyme assays using fluorescence-labeled N-glycopeptides as a substrate. In this study, we developed a new method for measuring aPNGase activity in crude extracts from plant materials
en-copyright=
kn-copyright=

en-aut-name=UemuraRyota
en-aut-sei=Uemura
en-aut-mei=Ryota
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OguraMikako
en-aut-sei=Ogura
en-aut-mei=Mikako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MatsumaruChihiro
en-aut-sei=Matsumaru
en-aut-mei=Chihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=AkiyamaTsuyoshi
en-aut-sei=Akiyama
en-aut-mei=Tsuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MaedaMegumi
en-aut-sei=Maeda
en-aut-mei=Megumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KimuraYoshinobu
en-aut-sei=Kimura
en-aut-mei=Yoshinobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
kn-affil=

affil-num=2
en-affil=Faculty of Agriculture , Okayama University
kn-affil=

affil-num=3
en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
kn-affil=

affil-num=4
en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
kn-affil=

affil-num=5
en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
kn-affil=

affil-num=6
en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
kn-affil=
en-keyword=Acidic PNGase
kn-keyword=Acidic PNGase
en-keyword=FNG: free N-glycan
kn-keyword=FNG: free N-glycan
en-keyword=Fuc: L-fucose
kn-keyword=Fuc: L-fucose
en-keyword=Gal: D-galactose
kn-keyword=Gal: D-galactose
en-keyword=GlcNAc: N-acetyl-D-glucosamine
kn-keyword=GlcNAc: N-acetyl-D-glucosamine
en-keyword=HPLC: high-performance liquid chromatography
kn-keyword=HPLC: high-performance liquid chromatography
en-keyword=Man: D-mannose
kn-keyword=Man: D-mannose
en-keyword=NeuNAc2Gal2GlcNAc2Man3GlcNAc1: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAc
kn-keyword=NeuNAc2Gal2GlcNAc2Man3GlcNAc1: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAc
en-keyword=NeuNAc2Gal2GlcNAc2Man3GlcNAc2: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAcβ1–4GlcNAc
kn-keyword=NeuNAc2Gal2GlcNAc2Man3GlcNAc2: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAcβ1–4GlcNAc
en-keyword=NeuNAc: N-acetylneuraminic acid
kn-keyword=NeuNAc: N-acetylneuraminic acid
en-keyword=PA-: pyridylamino
kn-keyword=PA-: pyridylamino
en-keyword=PNGase-A: aPNGase from almond seed
kn-keyword=PNGase-A: aPNGase from almond seed
en-keyword=PNGase-Le: aPNGase from tomato (Solanum lycopersium L.)
kn-keyword=PNGase-Le: aPNGase from tomato (Solanum lycopersium L.)
en-keyword=PNGase: peptide:N-glycanase
kn-keyword=PNGase: peptide:N-glycanase
en-keyword=PTC: plant complex type
kn-keyword=PTC: plant complex type
en-keyword=RCA120: Ricinus communis agglutinin (120 kDa)
kn-keyword=RCA120: Ricinus communis agglutinin (120 kDa)
en-keyword=RP-HPLC: reversed-phase HPLC
kn-keyword=RP-HPLC: reversed-phase HPLC
en-keyword=SF-HPLC: size-fractionation HPLC
kn-keyword=SF-HPLC: size-fractionation HPLC
en-keyword=Xyl: D-xylose
kn-keyword=Xyl: D-xylose
en-keyword=affinity chromatography
kn-keyword=affinity chromatography
en-keyword=enzyme assay
kn-keyword=enzyme assay
en-keyword=free N-glycans
kn-keyword=free N-glycans
en-keyword=transgenic plant
kn-keyword=transgenic plant
END